Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G7045_RS00890 Genome accession   NZ_CP049885
Coordinates   206273..207316 (-) Length   347 a.a.
NCBI ID   WP_166155995.1    Uniprot ID   A0A6G8AD24
Organism   Acidovorax sp. HDW3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 201273..212316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7045_RS00860 (G7045_00860) - 201740..202135 (+) 396 WP_166155983.1 YraN family protein -
  G7045_RS00865 (G7045_00865) - 202187..202783 (+) 597 WP_166155985.1 SIS domain-containing protein -
  G7045_RS00870 (G7045_00870) - 202783..203496 (+) 714 WP_166155987.1 BON domain-containing protein -
  G7045_RS00875 (G7045_00875) - 203521..204429 (-) 909 WP_166155989.1 NAD(P)-dependent oxidoreductase -
  G7045_RS00880 (G7045_00880) pilU 204432..205568 (-) 1137 WP_166155991.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G7045_RS00885 (G7045_00885) - 205614..206252 (-) 639 WP_166155993.1 cyclic nucleotide-binding domain-containing protein -
  G7045_RS00890 (G7045_00890) pilT 206273..207316 (-) 1044 WP_166155995.1 type IV pilus twitching motility protein PilT Machinery gene
  G7045_RS00895 (G7045_00895) - 207411..208136 (+) 726 WP_166155997.1 YggS family pyridoxal phosphate-dependent enzyme -
  G7045_RS14865 - 208283..208345 (+) 63 Protein_174 zinc-ribbon domain-containing protein -
  G7045_RS00900 (G7045_00900) - 208535..208966 (+) 432 WP_166155999.1 hypothetical protein -
  G7045_RS00905 (G7045_00905) - 209012..209782 (+) 771 WP_166156001.1 zinc ribbon domain-containing protein -
  G7045_RS00910 (G7045_00910) - 209859..210992 (+) 1134 WP_166156003.1 aminotransferase class V-fold PLP-dependent enzyme -
  G7045_RS00915 (G7045_00915) - 211017..211442 (-) 426 WP_166156005.1 XRE family transcriptional regulator -
  G7045_RS00920 (G7045_00920) - 211661..211807 (+) 147 WP_166156007.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38122.96 Da        Isoelectric Point: 6.8393

>NTDB_id=428318 G7045_RS00890 WP_166155995.1 206273..207316(-) (pilT) [Acidovorax sp. HDW3]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRLNVDPLDHKTVHAMVYDIMSDSQRKMYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVLRTIPSKILTLEQLNAPKIFADLALKPRGLVLVTGPTGSGKSTTLAGMVNHLNETEYGHILTIED
PIEFVHESKKSLINQREVGPMTLSFAAALKSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKLKDGSGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTGNSVGMQTLDQN
LTDLVRRNIISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=428318 G7045_RS00890 WP_166155995.1 206273..207316(-) (pilT) [Acidovorax sp. HDW3]
GTGGACATCACCCAACTGCTGGCGTTCAGCGTCAAGAACAAGGCTTCCGACTTGCACCTGTCGGCGGGCCTGCCACCCAT
GATCCGCGTGCACGGCGACGTGCGCCGCCTGAACGTCGATCCACTCGACCACAAGACGGTGCACGCCATGGTGTACGACA
TCATGAGCGACTCGCAGCGCAAGATGTACGAAGAATTCCTGGAGGTGGACTTTTCTTTCGAGATCGAGGGCCTGGCGCGC
TTTCGCGTCAACGCCTTCAACCAGAACCGGGGCGCCGCCGCCGTCTTGCGAACGATTCCAAGCAAAATCCTGACGCTCGA
ACAGCTCAATGCACCGAAAATTTTTGCCGACCTGGCACTCAAGCCGCGCGGCCTGGTGCTGGTGACGGGGCCCACGGGTT
CGGGCAAATCGACCACGCTCGCAGGCATGGTCAACCACCTCAACGAAACCGAGTACGGCCACATCCTCACCATCGAGGAT
CCGATCGAATTCGTGCACGAGTCGAAAAAATCCCTGATCAACCAGCGCGAAGTCGGGCCGATGACGCTGTCGTTCGCCGC
CGCCCTCAAGTCCGCGCTGCGCGAAGACCCGGACGCCATTTTGGTGGGCGAACTGCGTGACCTGGAAACCATCCGCCTGG
CCATGACGGCGGCGGAAACCGGCCACTTGGTGTTTGGCACCTTGCACACCAGCAGCGCCGCCAAAACCATCGACCGCATC
ATCGACGTCTTCCCAGCGGAAGAAAAAGAGATGGTGCGCGCCATGCTGTCCGAATCGCTGCAGGCCGTGATCTCGCAAAC
GCTGTGCAAGCTCAAGGACGGTTCAGGCCGCGTTGCCGCGCACGAAATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACTGGCAACAGCGTTGGTATGCAAACGCTCGACCAGAAC
CTCACCGACCTGGTGCGGCGCAACATCATCAGCCCGGCCGAAGCGCGCAGCAAGGCCAAGATTCCCGAGAACTTCCCCGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8AD24

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

74.783

99.424

0.744
  pilT Acinetobacter nosocomialis M2

74.493

99.424

0.741
  pilT Acinetobacter baumannii D1279779

74.493

99.424

0.741
  pilT Acinetobacter baumannii strain A118

74.493

99.424

0.741
  pilT Acinetobacter baylyi ADP1

73.333

99.424

0.729
  pilT Legionella pneumophila strain Lp02

72.861

97.695

0.712
  pilT Legionella pneumophila strain ERS1305867

72.861

97.695

0.712
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Neisseria meningitidis 8013

67.246

99.424

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.155

98.271

0.66
  pilT Vibrio cholerae strain A1552

67.155

98.271

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398