Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   G7045_RS00880 Genome accession   NZ_CP049885
Coordinates   204432..205568 (-) Length   378 a.a.
NCBI ID   WP_166155991.1    Uniprot ID   A0A6G8ACZ7
Organism   Acidovorax sp. HDW3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 199432..210568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7045_RS00855 (G7045_00855) rsmI 200808..201740 (-) 933 WP_166155981.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  G7045_RS00860 (G7045_00860) - 201740..202135 (+) 396 WP_166155983.1 YraN family protein -
  G7045_RS00865 (G7045_00865) - 202187..202783 (+) 597 WP_166155985.1 SIS domain-containing protein -
  G7045_RS00870 (G7045_00870) - 202783..203496 (+) 714 WP_166155987.1 BON domain-containing protein -
  G7045_RS00875 (G7045_00875) - 203521..204429 (-) 909 WP_166155989.1 NAD(P)-dependent oxidoreductase -
  G7045_RS00880 (G7045_00880) pilU 204432..205568 (-) 1137 WP_166155991.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G7045_RS00885 (G7045_00885) - 205614..206252 (-) 639 WP_166155993.1 cyclic nucleotide-binding domain-containing protein -
  G7045_RS00890 (G7045_00890) pilT 206273..207316 (-) 1044 WP_166155995.1 type IV pilus twitching motility protein PilT Machinery gene
  G7045_RS00895 (G7045_00895) - 207411..208136 (+) 726 WP_166155997.1 YggS family pyridoxal phosphate-dependent enzyme -
  G7045_RS14865 - 208283..208345 (+) 63 Protein_174 zinc-ribbon domain-containing protein -
  G7045_RS00900 (G7045_00900) - 208535..208966 (+) 432 WP_166155999.1 hypothetical protein -
  G7045_RS00905 (G7045_00905) - 209012..209782 (+) 771 WP_166156001.1 zinc ribbon domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41699.08 Da        Isoelectric Point: 6.8391

>NTDB_id=428317 G7045_RS00880 WP_166155991.1 204432..205568(-) (pilU) [Acidovorax sp. HDW3]
MERDQASKFINDLLKLMVSRGGSDLFLTAEFPPAIKIDGKVTKVSPQPLTPAHTLTLARSIMSDKQVADFERSKECNFAI
SPAGIGRFRVNAFIQQGRVGLVLRTIPLTLPTIDGLGVPQVLKEVAMTKRGLCIMVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRVINFFPEERRAQLLMDLSLNLRAMVSQRLLPKQDGKGRAAAVEVMLNTPLISDLIFKGEVSEIKEIMKKSRNLG
MQTFDQALFDLFEANVITYEDALRNADSLNDLRLQIKLSSQRAKSSDLASGTENFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=428317 G7045_RS00880 WP_166155991.1 204432..205568(-) (pilU) [Acidovorax sp. HDW3]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGACCTGCTCAAGCTCATGGTCAGCCGGGGCGGCAGCGACCTGTTCTT
GACGGCCGAATTCCCGCCCGCCATCAAGATCGACGGCAAGGTCACCAAAGTCTCGCCGCAGCCGCTCACGCCTGCGCACA
CCCTGACGCTGGCGCGCTCCATCATGAGCGACAAGCAGGTGGCCGATTTCGAGCGCTCCAAGGAGTGCAACTTCGCCATC
TCGCCCGCCGGTATCGGGCGCTTTCGCGTCAACGCCTTCATCCAACAGGGGCGCGTGGGCCTGGTGCTGCGTACCATCCC
ACTGACGCTGCCGACCATCGACGGCCTGGGCGTGCCCCAGGTGCTCAAGGAAGTGGCCATGACCAAGCGTGGCCTGTGCA
TCATGGTTGGCGCCACCGGCTCGGGCAAATCGACGACGCTCGCCGCCATGGTCGATTGGCGCAACGAAAACTCGTTCGGC
CACATCATCACCGTGGAAGATCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCGTCGTCACGCAGCGCGAGGTCGGGCT
CGACACCGACAGCTGGGAAGCGGCGCTGAAAAACACCCTGCGCCAGGCACCCGACGTCATCTTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAGCACGCGGTGGCGTTTGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCACTCGACCGCGTCATCAACTTCTTCCCTGAGGAGCGCCGCGCGCAGCTGCTGATGGACTTGTCGCTGAACCT
GCGCGCCATGGTCTCGCAGCGCCTCCTGCCCAAGCAGGACGGCAAGGGCCGCGCCGCCGCCGTCGAAGTGATGCTGAACA
CGCCGCTCATTTCTGACCTGATCTTCAAGGGCGAAGTCTCGGAGATCAAAGAGATCATGAAAAAAAGCCGCAACCTCGGG
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGGCCAACGTCATCACCTACGAAGATGCGCTGCGCAACGCCGA
CTCGCTCAACGACCTGCGCCTGCAGATCAAGCTCTCAAGCCAGCGCGCCAAAAGCTCTGACCTGGCTTCCGGCACGGAAA
ACTTCGCCATCGTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8ACZ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

57.542

94.709

0.545
  pilU Vibrio cholerae strain A1552

51.791

96.032

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas aeruginosa PAK

44.807

89.153

0.399
  pilT Acinetobacter baumannii strain A118

44.745

88.095

0.394
  pilT Acinetobacter nosocomialis M2

44.745

88.095

0.394
  pilT Acinetobacter baumannii D1279779

44.745

88.095

0.394
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Legionella pneumophila strain Lp02

43.544

88.095

0.384
  pilT Legionella pneumophila strain ERS1305867

43.544

88.095

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.47

87.831

0.373
  pilT Vibrio cholerae strain A1552

42.47

87.831

0.373
  pilT Acinetobacter baylyi ADP1

42.683

86.772

0.37