Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G7069_RS05830 Genome accession   NZ_CP049864
Coordinates   1232478..1233515 (+) Length   345 a.a.
NCBI ID   WP_166295236.1    Uniprot ID   -
Organism   Lysobacter sp. HDW10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1227478..1238515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7069_RS05800 (G7069_05800) - 1228372..1228893 (-) 522 WP_166295226.1 shikimate kinase -
  G7069_RS05805 (G7069_05805) pdxH 1228968..1229564 (+) 597 WP_166295228.1 pyridoxamine 5'-phosphate oxidase -
  G7069_RS05810 (G7069_05810) - 1229564..1230364 (+) 801 WP_166297590.1 ABC transporter substrate-binding protein -
  G7069_RS05815 (G7069_05815) - 1230353..1230841 (-) 489 WP_166295230.1 DUF4426 domain-containing protein -
  G7069_RS05820 (G7069_05820) proC 1230849..1231682 (-) 834 WP_166295232.1 pyrroline-5-carboxylate reductase -
  G7069_RS05825 (G7069_05825) - 1231701..1232372 (-) 672 WP_166295234.1 YggS family pyridoxal phosphate-dependent enzyme -
  G7069_RS05830 (G7069_05830) pilT 1232478..1233515 (+) 1038 WP_166295236.1 type IV pilus twitching motility protein PilT Machinery gene
  G7069_RS05835 (G7069_05835) pilU 1233542..1234675 (+) 1134 WP_166295238.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G7069_RS05840 (G7069_05840) - 1234711..1235274 (+) 564 WP_166295240.1 YqgE/AlgH family protein -
  G7069_RS05845 (G7069_05845) ruvX 1235271..1235723 (+) 453 WP_166295242.1 Holliday junction resolvase RuvX -
  G7069_RS05850 (G7069_05850) - 1235734..1236657 (+) 924 WP_166295244.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38568.24 Da        Isoelectric Point: 6.5760

>NTDB_id=428243 G7069_RS05830 WP_166295236.1 1232478..1233515(+) (pilT) [Lysobacter sp. HDW10]
MDIAELLAFSVKHKASDLHLSAGLPPMIRVDGDVRRINIAPLEHKEVHALIYDIMSDKQRRDYEEFLETDFSFEIPGLAR
FRVNAFTQNRGSGAVFRTIPSVVLSLEELGCPPVFRELVQQPQGLILVTGPTGSGKSTTLAAMLDYINRNEYGHILSVED
PIEFVHQSQKCLVNQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVLGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQSLLKKVGGGRIAAHEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQCL
QDLVARNLVIKQQAREYAKDKRLFD

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=428243 G7069_RS05830 WP_166295236.1 1232478..1233515(+) (pilT) [Lysobacter sp. HDW10]
ATGGATATCGCTGAACTCCTGGCCTTTTCGGTCAAGCACAAAGCGTCCGATTTGCATCTTTCGGCGGGCTTGCCACCGAT
GATCCGGGTGGACGGTGACGTTCGACGCATCAATATTGCGCCGCTCGAACACAAAGAAGTGCACGCGCTGATTTACGACA
TCATGTCGGACAAGCAACGCCGTGATTACGAAGAATTTCTCGAAACCGACTTTTCGTTTGAAATCCCCGGCTTGGCACGC
TTCCGCGTCAATGCCTTTACACAGAACCGCGGCTCGGGTGCGGTGTTCCGTACGATTCCGTCCGTCGTTTTGAGTTTGGA
AGAACTGGGCTGTCCGCCCGTGTTTCGTGAGCTCGTTCAACAGCCGCAAGGTTTGATTCTGGTGACCGGGCCCACCGGTT
CGGGTAAGTCGACCACGTTGGCTGCCATGCTCGACTACATCAATCGCAACGAGTACGGCCACATTCTGTCGGTGGAAGAT
CCGATCGAATTCGTTCACCAATCGCAAAAATGCTTGGTCAATCAGCGCGAAGTGCACCGCGATACGCACGGCTTCAACGA
AGCACTGCGTTCGGCCTTGCGTGAAGATCCTGACTACATTCTGGTCGGCGAATTGCGTGACCTTGAAACCATTCGCTTGG
CATTGACCGCTGCAGAAACGGGGCACTTGGTGTTGGGCACCTTGCATACCTCAAGTGCCGCCAAAACCATCGACCGGATC
ATCGACGTGTTCCCCGCCGGCGAAAAGCCGATGGTGCGTTCAATGTTGTCCGAATCGCTCCGTGCCGTTATTTCACAAAG
TCTGTTGAAAAAAGTGGGCGGCGGACGTATCGCGGCGCACGAAATCATGGTGGGCACGCCCGCCATTCGAAACCTCATTC
GCGAAGACAAAGTCGCGCAGATGTACTCGTCGATCCAAACCGGTCAGCAGTACGGCATGCAAACGCTTGATCAGTGCTTA
CAAGATTTGGTGGCGCGTAATCTCGTCATCAAGCAGCAAGCGCGCGAATACGCAAAAGATAAGCGCCTGTTCGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

79.646

98.261

0.783
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Acinetobacter baumannii D1279779

77.907

99.71

0.777
  pilT Acinetobacter baumannii strain A118

77.907

99.71

0.777
  pilT Acinetobacter nosocomialis M2

77.616

99.71

0.774
  pilT Acinetobacter baylyi ADP1

77.035

99.71

0.768
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.851

97.101

0.678
  pilT Vibrio cholerae strain A1552

69.851

97.101

0.678
  pilT Neisseria meningitidis 8013

64.516

98.841

0.638
  pilT Neisseria gonorrhoeae MS11

64.223

98.841

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.696

100

0.487
  pilU Vibrio cholerae strain A1552

41.092

100

0.414
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.308

94.203

0.38