Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   G7069_RS05835 Genome accession   NZ_CP049864
Coordinates   1233542..1234675 (+) Length   377 a.a.
NCBI ID   WP_166295238.1    Uniprot ID   A0A6G7YWN8
Organism   Lysobacter sp. HDW10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1228542..1239675
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7069_RS05805 (G7069_05805) pdxH 1228968..1229564 (+) 597 WP_166295228.1 pyridoxamine 5'-phosphate oxidase -
  G7069_RS05810 (G7069_05810) - 1229564..1230364 (+) 801 WP_166297590.1 ABC transporter substrate-binding protein -
  G7069_RS05815 (G7069_05815) - 1230353..1230841 (-) 489 WP_166295230.1 DUF4426 domain-containing protein -
  G7069_RS05820 (G7069_05820) proC 1230849..1231682 (-) 834 WP_166295232.1 pyrroline-5-carboxylate reductase -
  G7069_RS05825 (G7069_05825) - 1231701..1232372 (-) 672 WP_166295234.1 YggS family pyridoxal phosphate-dependent enzyme -
  G7069_RS05830 (G7069_05830) pilT 1232478..1233515 (+) 1038 WP_166295236.1 type IV pilus twitching motility protein PilT Machinery gene
  G7069_RS05835 (G7069_05835) pilU 1233542..1234675 (+) 1134 WP_166295238.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G7069_RS05840 (G7069_05840) - 1234711..1235274 (+) 564 WP_166295240.1 YqgE/AlgH family protein -
  G7069_RS05845 (G7069_05845) ruvX 1235271..1235723 (+) 453 WP_166295242.1 Holliday junction resolvase RuvX -
  G7069_RS05850 (G7069_05850) - 1235734..1236657 (+) 924 WP_166295244.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42078.21 Da        Isoelectric Point: 6.5053

>NTDB_id=428244 G7069_RS05835 WP_166295238.1 1233542..1234675(+) (pilU) [Lysobacter sp. HDW10]
MTTPTDFTGFLKIMAQQRASDLFITAGMPPSYKVHGKMTPIIETPLTPQESRDIVLSIMSPAQREEFERTKECNFAIGLA
GTGRFRVSAFYQRNQVGMVLRRIESKIPTIEELNLPPVMQSLAMTKRGIVMFVGATGSGKSTSLASLVGYRNKNSSGHII
TIEDPIEFVHRHEGCMVTQREVGIDTDSWESALKNTLRQAPDVILVGEVRTRETMDHAIAFAETGHLVLCTLHANNANQA
MDRIINFFPEDRRQQLLMDLSLNLKGVVAQQLVPTIDGKGRRVAIEVLLGTPLVQDYIRDGEIHKLKEVMKDSTQLGMRT
FDQSLFEMYQEGHISYDDALRFADSTNEVRLRIKLAQGGDPHSLAQGMDGVEVSESR

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=428244 G7069_RS05835 WP_166295238.1 1233542..1234675(+) (pilU) [Lysobacter sp. HDW10]
ATGACTACCCCCACCGATTTCACAGGCTTCCTCAAGATCATGGCGCAGCAACGCGCCTCCGACTTGTTTATCACCGCCGG
TATGCCGCCTTCTTACAAGGTGCACGGCAAGATGACGCCAATCATTGAAACGCCGCTAACGCCCCAAGAGTCGCGAGACA
TCGTGCTCAGCATCATGTCGCCGGCACAGCGTGAAGAGTTCGAACGCACGAAGGAATGCAACTTCGCGATCGGTCTCGCC
GGTACGGGCCGTTTTCGTGTGTCGGCGTTCTACCAGCGCAACCAAGTGGGCATGGTCTTACGTCGGATTGAAAGCAAGAT
TCCGACCATTGAAGAATTGAACTTGCCGCCGGTCATGCAAAGCTTGGCGATGACCAAGCGCGGCATCGTGATGTTCGTGG
GTGCCACCGGTTCGGGTAAATCAACCTCCTTGGCATCCTTGGTGGGTTACCGCAATAAGAACTCAAGTGGCCACATCATC
ACGATTGAAGATCCGATTGAATTCGTGCATCGCCACGAAGGCTGCATGGTCACCCAGCGCGAAGTCGGCATCGATACCGA
TAGCTGGGAATCCGCGCTGAAGAACACGTTGCGCCAAGCGCCAGACGTGATTTTGGTCGGCGAAGTGCGTACGCGCGAAA
CCATGGATCACGCGATTGCATTCGCAGAAACGGGTCACTTGGTGCTGTGTACGCTGCACGCCAACAATGCGAACCAAGCC
ATGGATCGCATCATCAACTTCTTCCCTGAAGACCGCCGTCAACAATTGTTGATGGATCTCTCTTTGAACTTGAAGGGTGT
GGTGGCGCAGCAGCTGGTGCCGACCATTGACGGTAAAGGCCGTCGCGTCGCGATCGAAGTGTTGTTGGGCACACCGTTGG
TGCAGGACTACATCCGCGATGGCGAGATCCACAAGCTGAAGGAAGTGATGAAAGACTCCACGCAGCTCGGTATGCGGACG
TTCGACCAAAGCCTGTTCGAGATGTACCAAGAAGGGCACATTTCTTACGACGACGCACTGCGTTTTGCCGACTCGACCAA
CGAAGTACGTCTGCGCATCAAGCTTGCCCAAGGCGGCGATCCGCATTCGTTGGCGCAAGGCATGGACGGGGTCGAGGTTT
CCGAGAGCCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G7YWN8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.94

97.082

0.65
  pilU Acinetobacter baylyi ADP1

62.079

94.43

0.586
  pilU Vibrio cholerae strain A1552

54.085

94.164

0.509
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.44

90.981

0.395
  pilT Legionella pneumophila strain Lp02

41.317

88.594

0.366
  pilT Legionella pneumophila strain ERS1305867

41.317

88.594

0.366
  pilT Pseudomonas aeruginosa PAK

41.018

88.594

0.363