Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DSC_RS12170 Genome accession   NC_016147
Coordinates   2681427..2682464 (-) Length   345 a.a.
NCBI ID   WP_014161467.1    Uniprot ID   G7USP3
Organism   Pseudoxanthomonas spadix BD-a59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2676427..2687464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSC_RS12155 (DSC_13235) - 2677382..2679226 (-) 1845 WP_014161464.1 copper resistance system multicopper oxidase -
  DSC_RS12160 (DSC_13240) - 2679355..2679780 (-) 426 WP_014161465.1 CopL family metal-binding regulatory protein -
  DSC_RS12165 (DSC_13245) pilU 2680140..2681270 (-) 1131 WP_014161466.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DSC_RS12170 (DSC_13250) pilT 2681427..2682464 (-) 1038 WP_014161467.1 type IV pilus twitching motility protein PilT Machinery gene
  DSC_RS12175 (DSC_13255) - 2682633..2683268 (+) 636 WP_238527750.1 YggS family pyridoxal phosphate-dependent enzyme -
  DSC_RS12180 (DSC_13265) proC 2683319..2684173 (+) 855 WP_043290625.1 pyrroline-5-carboxylate reductase -
  DSC_RS12185 (DSC_13270) pdxH 2684613..2685221 (-) 609 WP_043291337.1 pyridoxamine 5'-phosphate oxidase -
  DSC_RS12190 (DSC_13275) - 2685271..2686092 (+) 822 WP_014161472.1 hypothetical protein -
  DSC_RS12195 (DSC_13280) - 2686101..2686541 (-) 441 WP_014161473.1 hypothetical protein -
  DSC_RS12200 (DSC_13285) - 2686541..2686846 (-) 306 WP_014161474.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38713.38 Da        Isoelectric Point: 6.6654

>NTDB_id=42739 DSC_RS12170 WP_014161467.1 2681427..2682464(-) (pilT) [Pseudoxanthomonas spadix BD-a59]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLSLEDLGCPPLFREMIQQPQGLILVTGPTGSGKSTTLAAMIDFINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRSVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSQVTRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=42739 DSC_RS12170 WP_014161467.1 2681427..2682464(-) (pilT) [Pseudoxanthomonas spadix BD-a59]
ATGGATATCGCCGAACTACTGGCCTTCTCGGTCAAGAACAAGGCCTCGGACCTGCATCTGTCCGCCGGCCTGCCACCGAT
GATCCGGGTGGACGGCGACGTTCGCCGCATCAACATCCCCGCGCTGGACCACAAGCAGGTCCACGCCCTTGTCTACGACA
TCATGTCCGACAAGCAGCGCCGCGATTATGAAGAGTTCCTCGAGGTCGACTTCTCCTTCGAGATTCCCTCGCTGGCCCGC
TTCCGGGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGGACCATCCCCTCCGAGGTGCTGAGCCTGGA
GGACTTGGGCTGCCCGCCGCTGTTCCGCGAGATGATCCAGCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCGATGATCGACTTCATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCACGGCTTCAACGA
GGCGCTGCGCTCGGCCCTGCGCGAGGACCCCGACTACATCCTGGTCGGCGAGCTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCCGAAACCGGCCACCTGGTGTTCGGCACCTTGCACACCTCGTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTCTTTCCTGCCGGCGAAAAGCCGATGGTCCGCTCGATGCTGTCCGAGTCGCTGCGCTCGGTGATCTCCCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGCACCGCGGCCTGGGAGATCATGGTCGGCACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCAAGTCACCCGCAACCAGGCCAGGGAATACGCCAAGGACAAGCGGTTGTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G7USP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.488

99.71

0.783
  pilT Acinetobacter baumannii D1279779

78.488

99.71

0.783
  pilT Acinetobacter baumannii strain A118

78.488

99.71

0.783
  pilT Pseudomonas stutzeri DSM 10701

77.035

99.71

0.768
  pilT Pseudomonas aeruginosa PAK

77.876

98.261

0.765
  pilT Acinetobacter baylyi ADP1

76.744

99.71

0.765
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.213

95.362

0.67
  pilT Vibrio cholerae strain A1552

70.213

95.362

0.67
  pilT Neisseria meningitidis 8013

64.809

98.841

0.641
  pilT Neisseria gonorrhoeae MS11

64.516

98.841

0.638
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.696

100

0.487
  pilU Vibrio cholerae strain A1552

39.08

100

0.394
  pilU Acinetobacter baylyi ADP1

39.412

98.551

0.388
  pilU Pseudomonas stutzeri DSM 10701

39.233

98.261

0.386

Multiple sequence alignment