Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DSC_RS12165 Genome accession   NC_016147
Coordinates   2680140..2681270 (-) Length   376 a.a.
NCBI ID   WP_014161466.1    Uniprot ID   G7USP2
Organism   Pseudoxanthomonas spadix BD-a59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2675140..2686270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSC_RS12145 (DSC_13225) - 2675744..2676238 (-) 495 WP_014161462.1 nuclear transport factor 2 family protein -
  DSC_RS12150 (DSC_13230) - 2676249..2677385 (-) 1137 WP_014161463.1 copper resistance protein B -
  DSC_RS12155 (DSC_13235) - 2677382..2679226 (-) 1845 WP_014161464.1 copper resistance system multicopper oxidase -
  DSC_RS12160 (DSC_13240) - 2679355..2679780 (-) 426 WP_014161465.1 CopL family metal-binding regulatory protein -
  DSC_RS12165 (DSC_13245) pilU 2680140..2681270 (-) 1131 WP_014161466.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DSC_RS12170 (DSC_13250) pilT 2681427..2682464 (-) 1038 WP_014161467.1 type IV pilus twitching motility protein PilT Machinery gene
  DSC_RS12175 (DSC_13255) - 2682633..2683268 (+) 636 WP_238527750.1 YggS family pyridoxal phosphate-dependent enzyme -
  DSC_RS12180 (DSC_13265) proC 2683319..2684173 (+) 855 WP_043290625.1 pyrroline-5-carboxylate reductase -
  DSC_RS12185 (DSC_13270) pdxH 2684613..2685221 (-) 609 WP_043291337.1 pyridoxamine 5'-phosphate oxidase -
  DSC_RS12190 (DSC_13275) - 2685271..2686092 (+) 822 WP_014161472.1 hypothetical protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41953.26 Da        Isoelectric Point: 6.8812

>NTDB_id=42738 DSC_RS12165 WP_014161466.1 2680140..2681270(-) (pilU) [Pseudoxanthomonas spadix BD-a59]
MSTIDFTSFLKLMAHQKASDLFITSGMPPSIKVHGKLSPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPSIDELNLPPVVKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLVPTPDGKGRRVAMEIMLGTPLIQDYIREGEIHKLKEVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGLDGVEVAEMR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=42738 DSC_RS12165 WP_014161466.1 2680140..2681270(-) (pilU) [Pseudoxanthomonas spadix BD-a59]
ATGAGCACCATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCTCGGGCAT
GCCGCCCTCGATCAAGGTGCACGGCAAGCTCTCGCCGATCACCCAGACGCCGCTGACCCCGCAGCAGTCGCGCGACCTGG
TGCTCAACGTGATGACCCCGGCCCAGCGCGAGGAGTTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGC
GTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACCAGGATCCC
CAGCATCGACGAGCTGAACCTGCCGCCGGTGGTCAAGACCCTGGCCATGACCAAGCGCGGCATCATCCTGTTCGTGGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGCCACATCATCACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGATACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCATGCGATCTCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCATGCCAACAACGCCAACCAGGCGATG
GACCGGATCATCAATTTCTTCCCCGAAGACCGCCGCAACCAGCTGCTGATGGACCTGTCGCTGAACCTCAAGGGCGTGGT
CGCCCAGCAGCTGGTGCCCACGCCGGACGGCAAGGGCCGCCGGGTGGCGATGGAGATCATGCTGGGCACGCCGCTGATCC
AGGACTACATCCGCGAAGGGGAGATCCACAAGCTCAAGGAGGTCATGAAGGAATCGACCAACTTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAGATCTCCTACGAGGACGCGCTGCGCCATGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGATGCCCGCACCCTGGCGCAGGGCCTGGACGGGGTCGAAGTGGCCG
AAATGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G7USP2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

97.606

0.662
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

54.494

94.681

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

93.085

0.399
  pilT Acinetobacter baumannii strain A118

40.698

91.489

0.372
  pilT Acinetobacter nosocomialis M2

40.698

91.489

0.372
  pilT Acinetobacter baumannii D1279779

40.698

91.489

0.372
  pilT Legionella pneumophila strain Lp02

41.493

89.096

0.37
  pilT Legionella pneumophila strain ERS1305867

41.493

89.096

0.37
  pilT Pseudomonas aeruginosa PAK

41.003

90.16

0.37

Multiple sequence alignment