Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G5S32_RS12660 Genome accession   NZ_CP049331
Coordinates   2750453..2751490 (-) Length   345 a.a.
NCBI ID   WP_165312337.1    Uniprot ID   A0A6G7CL62
Organism   Vibrio ziniensis strain ZWAL4003     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2745453..2756490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5S32_RS12635 (G5S32_12635) rsmE 2746203..2746934 (+) 732 WP_165312333.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  G5S32_RS12640 (G5S32_12640) gshB 2746947..2747903 (+) 957 WP_165312334.1 glutathione synthase -
  G5S32_RS12645 (G5S32_12645) - 2747930..2748493 (+) 564 WP_165312335.1 YqgE/AlgH family protein -
  G5S32_RS12650 (G5S32_12650) ruvX 2748686..2749111 (+) 426 WP_042489189.1 Holliday junction resolvase RuvX -
  G5S32_RS12655 (G5S32_12655) pilU 2749335..2750441 (-) 1107 WP_165312336.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G5S32_RS12660 (G5S32_12660) pilT 2750453..2751490 (-) 1038 WP_165312337.1 type IV pilus twitching motility protein PilT Machinery gene
  G5S32_RS12665 (G5S32_12665) - 2751535..2752239 (+) 705 WP_165312338.1 YggS family pyridoxal phosphate-dependent enzyme -
  G5S32_RS12670 (G5S32_12670) proC 2752335..2753153 (+) 819 WP_165312339.1 pyrroline-5-carboxylate reductase -
  G5S32_RS12675 (G5S32_12675) - 2753182..2753739 (+) 558 WP_165312340.1 YggT family protein -
  G5S32_RS12680 (G5S32_12680) yggU 2753739..2754029 (+) 291 WP_165312341.1 DUF167 family protein YggU -
  G5S32_RS12685 (G5S32_12685) - 2754116..2754547 (+) 432 WP_165312342.1 DUF4426 domain-containing protein -
  G5S32_RS12690 (G5S32_12690) - 2754607..2755203 (+) 597 WP_165312343.1 XTP/dITP diphosphatase -
  G5S32_RS12695 (G5S32_12695) hemW 2755203..2756375 (+) 1173 WP_165312344.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38225.77 Da        Isoelectric Point: 6.5035

>NTDB_id=426168 G5S32_RS12660 WP_165312337.1 2750453..2751490(-) (pilT) [Vibrio ziniensis strain ZWAL4003]
MNIAELLDFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHADVHRLVFEVMNDAQRSEFEEKLEVDFSFELPDVGR
FRVNAFHQARGCSAVFRTIPTVIPTLDQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINKNHNKHILTIED
PIEFVHSNDKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGNDKDMVRSMLSESLRAVIAQKLLKRTGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNS
KQLIAKGLVEPAEVQKKIESEISTF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=426168 G5S32_RS12660 WP_165312337.1 2750453..2751490(-) (pilT) [Vibrio ziniensis strain ZWAL4003]
ATGAATATCGCTGAGTTACTGGATTTTAGTGTAAAGCATAATGCGTCAGATCTACATCTTTCTGCAGGTGTTCCTCCTAT
GGTACGGATAGATGGAGAAGTAAGAAAGCTTGGCGTACCTGCTTTTACTCATGCGGATGTTCATCGCCTAGTGTTTGAAG
TAATGAATGATGCTCAGCGTAGTGAGTTTGAAGAGAAACTCGAAGTGGATTTTTCATTTGAACTGCCGGATGTTGGACGA
TTCCGTGTCAACGCTTTCCATCAGGCTCGTGGCTGCTCTGCTGTATTCCGTACTATCCCGACAGTGATTCCTACTCTTGA
CCAGTTAGATGCGCCAGAGATTTTCAGCAAAATTGCCAATTATGAAAAAGGTCTGGTTTTGGTGACTGGACCTACGGGTT
CTGGTAAATCAACCACCTTGGCAGCGATGGTTGATTACATCAACAAGAATCACAACAAGCATATTCTTACCATTGAAGAT
CCAATCGAATTCGTTCATAGCAACGATAAGTGTTTGATCAACCAACGTGAAGTTCACCGCGATACACACAGTTTCAAGAA
TGCACTTCGTTCTGCATTGCGTGAAGACCCAGACGTCATTTTGGTGGGTGAGTTGCGTGACCAAGAGACCATTAGTTTGG
CTCTGACTGCTGCGGAAACAGGACACCTAGTGTTCGGTACATTGCATACCAGTTCTGCGGCAAAAACCATTGACCGTATT
ATTGATGTGTTTCCGGGAAACGATAAAGACATGGTGCGTTCTATGTTGTCTGAATCATTACGTGCGGTGATTGCACAGAA
GCTGCTAAAACGTACTGGCGGTGGACGAGTTGCTTGCCATGAGATCATGTTAGCAACACCTGCAATTCGTAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACTGGTGCTGCGCATGGTATGCAGACGATGGAGCAAAATTCT
AAGCAGTTGATTGCTAAGGGGCTAGTAGAACCGGCTGAAGTACAGAAGAAGATCGAGTCTGAAATCTCTACTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G7CL62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

92.174

100

0.922
  pilT Vibrio cholerae strain A1552

92.174

100

0.922
  pilT Acinetobacter baumannii D1279779

71.903

95.942

0.69
  pilT Acinetobacter baumannii strain A118

71.903

95.942

0.69
  pilT Acinetobacter nosocomialis M2

71.601

95.942

0.687
  pilT Acinetobacter baylyi ADP1

71.125

95.362

0.678
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.681

0.67
  pilT Pseudomonas aeruginosa PAK

67.359

97.681

0.658
  pilT Legionella pneumophila strain Lp02

66.565

95.362

0.635
  pilT Legionella pneumophila strain ERS1305867

66.565

95.362

0.635
  pilT Neisseria meningitidis 8013

62.536

100

0.629
  pilT Neisseria gonorrhoeae MS11

62.248

100

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.121

95.652

0.499
  pilU Vibrio cholerae strain A1552

43.692

94.203

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.073

95.072

0.4
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371