Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   G5S32_RS12655 Genome accession   NZ_CP049331
Coordinates   2749335..2750441 (-) Length   368 a.a.
NCBI ID   WP_165312336.1    Uniprot ID   A0A6G7CL06
Organism   Vibrio ziniensis strain ZWAL4003     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2744335..2755441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5S32_RS12625 (G5S32_12625) - 2744590..2745093 (+) 504 WP_165312331.1 SprT family zinc-dependent metalloprotease -
  G5S32_RS12630 (G5S32_12630) endA 2745242..2745949 (+) 708 WP_165312332.1 deoxyribonuclease I -
  G5S32_RS12635 (G5S32_12635) rsmE 2746203..2746934 (+) 732 WP_165312333.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  G5S32_RS12640 (G5S32_12640) gshB 2746947..2747903 (+) 957 WP_165312334.1 glutathione synthase -
  G5S32_RS12645 (G5S32_12645) - 2747930..2748493 (+) 564 WP_165312335.1 YqgE/AlgH family protein -
  G5S32_RS12650 (G5S32_12650) ruvX 2748686..2749111 (+) 426 WP_042489189.1 Holliday junction resolvase RuvX -
  G5S32_RS12655 (G5S32_12655) pilU 2749335..2750441 (-) 1107 WP_165312336.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G5S32_RS12660 (G5S32_12660) pilT 2750453..2751490 (-) 1038 WP_165312337.1 type IV pilus twitching motility protein PilT Machinery gene
  G5S32_RS12665 (G5S32_12665) - 2751535..2752239 (+) 705 WP_165312338.1 YggS family pyridoxal phosphate-dependent enzyme -
  G5S32_RS12670 (G5S32_12670) proC 2752335..2753153 (+) 819 WP_165312339.1 pyrroline-5-carboxylate reductase -
  G5S32_RS12675 (G5S32_12675) - 2753182..2753739 (+) 558 WP_165312340.1 YggT family protein -
  G5S32_RS12680 (G5S32_12680) yggU 2753739..2754029 (+) 291 WP_165312341.1 DUF167 family protein YggU -
  G5S32_RS12685 (G5S32_12685) - 2754116..2754547 (+) 432 WP_165312342.1 DUF4426 domain-containing protein -
  G5S32_RS12690 (G5S32_12690) - 2754607..2755203 (+) 597 WP_165312343.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41334.32 Da        Isoelectric Point: 6.3910

>NTDB_id=426167 G5S32_RS12655 WP_165312336.1 2749335..2750441(-) (pilU) [Vibrio ziniensis strain ZWAL4003]
MELNHYLEAMTSLKASDLYITVGAPVLLRVDGEMRPQGEKLSQQEVFSLLHEMMDAERESEFKSSHEANFAIVRGNGRFR
VSAFFQRELPGAVIRRIETQIPTFEELNLPAVLQDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNCHRTGHILTVEDPI
EFVHEHQKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVGQQLIRDKNGKGRHGVFEILLNSPRVSDLIRKGELHELKSTMAKSKEIGMQTFDQALY
HLLVEDKITEQDAMHSADSANDLRMMLKTQRGDAPSSSSLANVKIDMN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=426167 G5S32_RS12655 WP_165312336.1 2749335..2750441(-) (pilU) [Vibrio ziniensis strain ZWAL4003]
ATGGAATTGAATCACTACCTTGAAGCGATGACCTCGCTAAAAGCGTCAGATTTGTACATCACCGTCGGCGCGCCGGTACT
GTTACGTGTCGATGGTGAGATGCGTCCGCAGGGGGAGAAGCTTTCACAGCAAGAGGTGTTTTCCTTATTACATGAAATGA
TGGATGCAGAACGCGAGTCTGAGTTTAAATCATCACACGAAGCGAATTTTGCCATTGTAAGAGGGAATGGGCGTTTCAGG
GTGAGTGCCTTTTTCCAGCGTGAGCTACCGGGCGCCGTGATTCGACGCATCGAAACACAAATTCCGACATTTGAAGAACT
CAATCTGCCTGCGGTGTTGCAGGATCTCTCGATTGCCAAGCGAGGCTTGGTGTTGGTCGTTGGTGCGACAGGCTCTGGTA
AATCCACCACCATGGCTGCAATGACAGGGTATCGTAATTGTCATCGAACAGGGCATATCTTAACGGTTGAAGACCCGATT
GAATTTGTTCACGAGCATCAAAAATGCATCGTCACCCAGCGTGAGGTTGGTTTGGATACTGAGAGCTACGAAGTAGCACT
GAAGAATTCCCTTCGCCAAGCGCCAGACATGATTTTGATTGGTGAAATCCGCAGTCGTGAAACCATGGAATACGCGATGA
CCTTTGCTGAAACGGGGCACTTGTGTATGGCAACCTTGCACGCTAACAACGCCAACCAAGCGTTAGAGCGTATTCTTCAC
CTAGTACCTAAAGAGCAGAAAGAACAGTTCTTGTTTGATTTGTCGATGAACTTGCGTGGCGTGGTAGGGCAGCAACTGAT
TCGTGATAAAAATGGTAAAGGTCGTCACGGTGTGTTTGAGATATTACTCAATTCACCTCGCGTATCAGATTTGATTCGCA
AAGGAGAGTTGCATGAGTTGAAATCGACGATGGCGAAGTCCAAAGAGATTGGCATGCAGACCTTCGATCAAGCGTTATAT
CATTTGCTGGTGGAAGATAAGATCACCGAGCAAGATGCAATGCACAGTGCTGACTCAGCCAATGACTTACGGATGATGCT
TAAAACTCAGCGTGGAGATGCTCCGAGTTCAAGTTCTCTCGCGAATGTGAAAATCGATATGAACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G7CL06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.106

99.728

0.829
  pilU Pseudomonas stutzeri DSM 10701

58.286

95.109

0.554
  pilU Acinetobacter baylyi ADP1

53.276

95.38

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

91.576

0.402
  pilT Acinetobacter baylyi ADP1

41.935

92.663

0.389
  pilT Legionella pneumophila strain Lp02

41.298

92.12

0.38
  pilT Legionella pneumophila strain ERS1305867

41.298

92.12

0.38
  pilT Pseudomonas aeruginosa PAK

41.003

92.12

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Acinetobacter baumannii D1279779

40.413

92.12

0.372
  pilT Acinetobacter baumannii strain A118

40.413

92.12

0.372
  pilT Acinetobacter nosocomialis M2

40.413

92.12

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.118

92.12

0.37