Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GNIT_RS13040 Genome accession   NC_016041
Coordinates   3040043..3041086 (-) Length   347 a.a.
NCBI ID   WP_014109714.1    Uniprot ID   G4QIC7
Organism   Brumicola nitratireducens FR1064     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3035043..3046086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNIT_RS13020 (GNIT_2739) ruvX 3035313..3035732 (+) 420 WP_014109709.1 Holliday junction resolvase RuvX -
  GNIT_RS13025 (GNIT_2740) - 3035764..3037908 (-) 2145 WP_014109710.1 sensor domain-containing diguanylate cyclase -
  GNIT_RS13030 (GNIT_2741) - 3038032..3038367 (-) 336 WP_014109711.1 DUF2007 domain-containing protein -
  GNIT_RS13035 (GNIT_2743) pilU 3038864..3039979 (-) 1116 WP_014109713.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GNIT_RS13040 (GNIT_2744) pilT 3040043..3041086 (-) 1044 WP_014109714.1 type IV pilus twitching motility protein PilT Machinery gene
  GNIT_RS13045 (GNIT_2745) - 3041170..3041859 (+) 690 WP_014109715.1 YggS family pyridoxal phosphate-dependent enzyme -
  GNIT_RS13050 (GNIT_2746) proC 3041971..3042792 (+) 822 WP_014109716.1 pyrroline-5-carboxylate reductase -
  GNIT_RS13055 (GNIT_2747) - 3042810..3043352 (+) 543 WP_014109717.1 YggT family protein -
  GNIT_RS13060 (GNIT_2748) - 3043445..3043921 (+) 477 WP_014109718.1 DUF4426 domain-containing protein -
  GNIT_RS13065 (GNIT_2749) yaaA 3043975..3044796 (-) 822 WP_014109719.1 peroxide stress protein YaaA -
  GNIT_RS13070 (GNIT_2750) tal 3044951..3045904 (+) 954 WP_014109720.1 transaldolase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38497.39 Da        Isoelectric Point: 6.8253

>NTDB_id=42535 GNIT_RS13040 WP_014109714.1 3040043..3041086(-) (pilT) [Brumicola nitratireducens FR1064]
MDINELLAFSVSNKASDLHLSAGLPPIIRVDGEMRRLNVEPLDHKQVHALIFSIMNDHQRKDYEENLECDFSFEIKDLSR
FRVNAFVQNRGAAAVLRTIPSDILTLDQLGAPEIFKQIINQPTGIVLVTGATGSGKSTTLAAMVDHINTHKREHILTIED
PIEFVHKNKMCVMNQREVHRDTKSFNNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKSMVRSMLSESLRAVISQTLMKKVGGGRVAAHEIMIGIPAIRNLIREDKVPQMYSVIQTGQQHGMQTMDQCL
QRLVAMGVITANDAASKSIDKAPSIGM

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=42535 GNIT_RS13040 WP_014109714.1 3040043..3041086(-) (pilT) [Brumicola nitratireducens FR1064]
TTGGACATTAACGAACTACTCGCTTTTAGTGTAAGCAACAAAGCCTCAGATTTACACCTATCTGCAGGATTGCCTCCCAT
TATCCGTGTGGATGGTGAAATGCGACGTCTCAATGTTGAACCACTTGACCACAAACAAGTGCATGCTTTGATTTTTAGCA
TCATGAATGACCACCAACGTAAAGATTACGAAGAAAACCTAGAATGCGATTTTTCTTTTGAAATAAAAGATTTATCTCGT
TTCAGGGTTAATGCTTTTGTGCAAAATCGCGGTGCTGCAGCTGTTTTAAGAACTATTCCAAGTGACATTCTAACACTAGA
CCAATTGGGTGCACCGGAAATATTTAAACAAATCATTAATCAGCCGACGGGCATTGTTTTGGTAACAGGTGCAACAGGCT
CAGGTAAAAGTACCACACTTGCTGCAATGGTTGACCATATAAACACGCATAAGCGAGAGCATATTCTCACCATTGAAGAT
CCAATAGAATTTGTGCACAAGAATAAGATGTGTGTAATGAATCAGCGTGAAGTGCATCGCGATACGAAAAGCTTTAATAA
CGCGTTGCGCTCGGCGCTGCGTGAAGACCCAGACGTTATTTTGGTCGGTGAATTACGAGATTTAGAGACTATCCGTCTTG
CAATATCAGCGGCTGAAACTGGTCACTTAGTCTTCGGAACGCTTCACACGAACTCAGCGCCAAAAACTATCGACCGTATT
ATTGACGTGTTCCCAGCGGAAGAAAAGTCGATGGTACGTTCAATGCTGTCTGAATCGTTGCGAGCGGTAATTTCACAAAC
CTTAATGAAAAAGGTTGGTGGCGGTCGAGTAGCTGCACATGAAATCATGATAGGTATTCCTGCCATTAGAAACCTCATAC
GTGAAGACAAAGTACCGCAGATGTATTCTGTGATTCAAACGGGACAGCAGCACGGCATGCAAACAATGGATCAATGTTTA
CAGCGCTTGGTTGCGATGGGCGTTATTACAGCCAATGATGCAGCATCTAAGTCGATAGATAAAGCGCCATCAATAGGTAT
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G4QIC7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

72.886

98.847

0.72
  pilT Acinetobacter baumannii D1279779

73.669

97.406

0.718
  pilT Acinetobacter baumannii strain A118

73.669

97.406

0.718
  pilT Pseudomonas aeruginosa PAK

73.373

97.406

0.715
  pilT Pseudomonas stutzeri DSM 10701

73.373

97.406

0.715
  pilT Acinetobacter nosocomialis M2

73.373

97.406

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.217

97.118

0.692
  pilT Vibrio cholerae strain A1552

71.217

97.118

0.692
  pilT Legionella pneumophila strain Lp02

69.883

98.559

0.689
  pilT Legionella pneumophila strain ERS1305867

69.883

98.559

0.689
  pilT Neisseria gonorrhoeae MS11

63.873

99.712

0.637
  pilT Neisseria meningitidis 8013

66.061

95.101

0.628
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.199

98.271

0.513
  pilU Vibrio cholerae strain A1552

42.09

96.542

0.406
  pilU Acinetobacter baylyi ADP1

41.964

96.83

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403

Multiple sequence alignment