Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GNIT_RS13035 Genome accession   NC_016041
Coordinates   3038864..3039979 (-) Length   371 a.a.
NCBI ID   WP_014109713.1    Uniprot ID   -
Organism   Brumicola nitratireducens FR1064     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3033864..3044979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNIT_RS13015 (GNIT_2738) - 3034734..3035312 (+) 579 WP_014109708.1 YqgE/AlgH family protein -
  GNIT_RS13020 (GNIT_2739) ruvX 3035313..3035732 (+) 420 WP_014109709.1 Holliday junction resolvase RuvX -
  GNIT_RS13025 (GNIT_2740) - 3035764..3037908 (-) 2145 WP_014109710.1 sensor domain-containing diguanylate cyclase -
  GNIT_RS13030 (GNIT_2741) - 3038032..3038367 (-) 336 WP_014109711.1 DUF2007 domain-containing protein -
  GNIT_RS13035 (GNIT_2743) pilU 3038864..3039979 (-) 1116 WP_014109713.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GNIT_RS13040 (GNIT_2744) pilT 3040043..3041086 (-) 1044 WP_014109714.1 type IV pilus twitching motility protein PilT Machinery gene
  GNIT_RS13045 (GNIT_2745) - 3041170..3041859 (+) 690 WP_014109715.1 YggS family pyridoxal phosphate-dependent enzyme -
  GNIT_RS13050 (GNIT_2746) proC 3041971..3042792 (+) 822 WP_014109716.1 pyrroline-5-carboxylate reductase -
  GNIT_RS13055 (GNIT_2747) - 3042810..3043352 (+) 543 WP_014109717.1 YggT family protein -
  GNIT_RS13060 (GNIT_2748) - 3043445..3043921 (+) 477 WP_014109718.1 DUF4426 domain-containing protein -
  GNIT_RS13065 (GNIT_2749) yaaA 3043975..3044796 (-) 822 WP_014109719.1 peroxide stress protein YaaA -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41141.21 Da        Isoelectric Point: 5.9755

>NTDB_id=42534 GNIT_RS13035 WP_014109713.1 3038864..3039979(-) (pilU) [Brumicola nitratireducens FR1064]
MLGLHQYLQDMVVHSASDLFITVGMPVSAKINGRMTPLTTMNLTEQEALELVHDTMNEKQKKSFADDKECNFAISIDGIG
RFRCSAFWQRDQAGMVIRRIVTQIPNADDLGLPPVLKDIIMSKKGLVLFVGGTGTGKSTSLAALIGHRNQHSHGHILTIE
DPIEFVHEHNNCVVTQREVGIDTHTFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIER
IMHLAPQNLHQKLRFDLSQNIRAIVAQQLVPTIDGKGRVAAIEILLNSPLVSDLIQRNEIGMLKEAMMKGKEQGMQSFDM
ALYELFKENRISEEQAMHNADSPNDLRLMIKLDSQEGTGLGSLSNVSFDMG

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=42534 GNIT_RS13035 WP_014109713.1 3038864..3039979(-) (pilU) [Brumicola nitratireducens FR1064]
ATGTTAGGTTTACATCAGTATTTACAAGACATGGTGGTGCACAGTGCATCAGATTTATTCATTACGGTAGGCATGCCAGT
CAGTGCTAAAATTAACGGACGCATGACACCGCTAACGACGATGAATTTAACTGAGCAAGAGGCGCTGGAGCTGGTTCACG
ATACAATGAATGAAAAGCAAAAAAAGAGCTTTGCTGATGACAAAGAGTGTAATTTTGCTATTTCGATAGACGGCATTGGT
CGTTTCAGGTGCAGTGCTTTTTGGCAGCGAGATCAAGCGGGAATGGTTATTCGCCGCATCGTGACACAAATTCCAAATGC
AGACGATTTAGGTTTACCACCTGTATTGAAAGACATCATCATGTCTAAAAAGGGCTTGGTGTTGTTCGTGGGCGGCACAG
GCACCGGCAAATCTACCTCTCTAGCAGCACTCATCGGGCATCGAAACCAGCACTCTCATGGGCATATCCTGACCATTGAG
GACCCAATTGAGTTTGTGCATGAACACAATAACTGCGTGGTTACTCAGCGTGAAGTAGGCATCGACACGCATACATTTGA
TGACGCGTTGAAGAGCTCATTGCGTCAGGCACCTGATGTTATTTTGATTGGTGAAATTCGCTCTATGGAGACCATGGAGT
ATGCAATGTCTTTCGCTGACACGGGTCATTTATGCGTCGCGACACTGCATGCGAATAATGCGAACCAAGCAATTGAGCGC
ATCATGCATTTAGCTCCACAAAACCTGCATCAGAAGCTGCGTTTCGATTTAAGTCAAAATATTCGTGCTATTGTTGCTCA
GCAATTGGTCCCCACTATCGATGGTAAAGGTCGAGTTGCTGCAATAGAGATCCTTTTGAATTCGCCGCTAGTGTCAGATT
TAATTCAGCGCAATGAGATAGGCATGCTTAAAGAAGCGATGATGAAAGGCAAAGAGCAGGGGATGCAGAGCTTTGACATG
GCTTTGTATGAGCTGTTTAAAGAAAATCGTATTTCCGAAGAGCAAGCGATGCATAATGCTGACTCACCGAATGATTTACG
CTTAATGATTAAGTTAGATAGTCAGGAGGGCACCGGTTTGGGCAGCTTGTCGAACGTATCATTCGATATGGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.287

97.574

0.569
  pilU Vibrio cholerae strain A1552

55.041

98.922

0.544
  pilU Acinetobacter baylyi ADP1

55.866

96.496

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.115

94.07

0.377
  pilT Legionella pneumophila strain Lp02

41.231

87.601

0.361
  pilT Legionella pneumophila strain ERS1305867

41.231

87.601

0.361

Multiple sequence alignment