Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NH8B_RS19445 Genome accession   NC_016002
Coordinates   4197819..4198856 (+) Length   345 a.a.
NCBI ID   WP_014088767.1    Uniprot ID   G2J259
Organism   Pseudogulbenkiania sp. NH8B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4192819..4203856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH8B_RS19415 (NH8B_3999) aroG 4193266..4194342 (-) 1077 WP_008955366.1 3-deoxy-7-phosphoheptulonate synthase AroG -
  NH8B_RS19420 (NH8B_4000) dksA 4194522..4194932 (-) 411 WP_008955367.1 RNA polymerase-binding protein DksA -
  NH8B_RS19425 (NH8B_4001) - 4195140..4195445 (-) 306 WP_014088764.1 c-type cytochrome -
  NH8B_RS19430 (NH8B_4002) - 4195519..4196100 (-) 582 WP_008955369.1 YggT family protein -
  NH8B_RS19435 (NH8B_4003) proC 4196102..4196905 (-) 804 WP_014088765.1 pyrroline-5-carboxylate reductase -
  NH8B_RS19440 (NH8B_4004) - 4196957..4197652 (-) 696 WP_014088766.1 YggS family pyridoxal phosphate-dependent enzyme -
  NH8B_RS19445 (NH8B_4005) pilT 4197819..4198856 (+) 1038 WP_014088767.1 type IV pilus twitching motility protein PilT Machinery gene
  NH8B_RS19450 (NH8B_4006) pilU 4199095..4200231 (+) 1137 WP_008955373.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NH8B_RS19455 (NH8B_4007) - 4200369..4201583 (-) 1215 WP_014088768.1 EAL and HDOD domain-containing protein -
  NH8B_RS19460 (NH8B_4008) thpR 4201617..4202153 (+) 537 WP_255345166.1 RNA 2',3'-cyclic phosphodiesterase -
  NH8B_RS19465 (NH8B_4009) - 4202397..4203131 (-) 735 WP_040653141.1 amino acid ABC transporter ATP-binding protein -
  NH8B_RS19470 (NH8B_4010) - 4203137..4203811 (-) 675 WP_014088770.1 amino acid ABC transporter permease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38508.35 Da        Isoelectric Point: 6.6631

>NTDB_id=42381 NH8B_RS19445 WP_014088767.1 4197819..4198856(+) (pilT) [Pseudogulbenkiania sp. NH8B]
MEISELLAFTVKNKASDLHLSAGLPPMIRVNGDVRRINLPPLEHHEVHDKVYDIMNDFQRKAFEEMMECDFSFELPGIAR
FRVNAFMQNRGMGAVFRVIPSKVLTLEELSAPKIFQEIASYPRGLVLVTGPTGSGKSTTLAAMIDYINDNEFSHILTVED
PIEFVHQSKRCMINQRELGQQTHSFANALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTVDRI
VDVFPAGEKEMVRSMLSESLRAVISQTLLKTKDGSGRVAAHEIMVGTAAIRNLIRENKIAQINSMIQTGQQYGMQTLDQC
LQELVRRNIASPAEARLKASNKDLF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=42381 NH8B_RS19445 WP_014088767.1 4197819..4198856(+) (pilT) [Pseudogulbenkiania sp. NH8B]
ATGGAAATTTCCGAGCTCTTGGCCTTCACCGTCAAGAACAAGGCGTCCGACCTGCACCTGTCGGCCGGCCTGCCGCCGAT
GATCCGGGTCAACGGCGACGTGCGCCGCATCAACCTGCCGCCGCTGGAGCACCACGAAGTGCACGACAAGGTGTACGACA
TCATGAACGACTTCCAGCGCAAGGCGTTCGAAGAAATGATGGAGTGCGACTTCTCGTTCGAGCTGCCCGGCATCGCCCGC
TTCCGGGTCAACGCCTTCATGCAGAACCGCGGCATGGGTGCCGTGTTCCGGGTGATTCCGTCCAAGGTGCTGACGCTGGA
GGAGCTGTCGGCGCCGAAGATCTTCCAGGAGATCGCCAGCTACCCGCGCGGCCTGGTGCTGGTGACCGGACCGACCGGCT
CCGGCAAGTCCACCACGCTGGCGGCGATGATCGACTATATCAACGATAATGAATTCTCCCACATTCTGACCGTGGAAGAC
CCGATCGAATTCGTGCACCAGAGCAAGCGCTGCATGATCAACCAGCGCGAACTGGGGCAGCAGACCCACAGTTTCGCCAA
CGCGTTGCGCTCGGCGCTGCGCGAAGACCCGGACGTGATCCTGGTGGGGGAAATGCGCGACCTGGAAACCATCCGCCTCG
CCATGACCGCGGCGGAAACCGGTCACCTGGTGTTCGGCACCTTGCACACCAGCTCGGCCGCCAAGACCGTCGACCGTATC
GTCGACGTATTCCCGGCCGGCGAGAAGGAGATGGTGCGCTCGATGCTGTCGGAGAGCCTGCGCGCGGTGATCTCGCAGAC
CCTGCTGAAGACCAAGGACGGCAGCGGACGGGTGGCGGCGCACGAGATCATGGTCGGCACGGCGGCGATCCGCAACCTGA
TCCGCGAGAACAAGATCGCCCAGATCAACTCGATGATCCAGACCGGCCAGCAGTACGGCATGCAGACGCTCGACCAGTGC
CTGCAGGAACTGGTACGCCGCAACATCGCGTCACCGGCCGAGGCACGGCTGAAGGCCAGCAACAAGGATTTGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G2J259

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

73.913

100

0.739
  pilT Acinetobacter baumannii D1279779

73.913

100

0.739
  pilT Acinetobacter baumannii strain A118

73.913

100

0.739
  pilT Pseudomonas aeruginosa PAK

73.746

98.261

0.725
  pilT Neisseria meningitidis 8013

72.595

99.42

0.722
  pilT Neisseria gonorrhoeae MS11

72.303

99.42

0.719
  pilT Legionella pneumophila strain Lp02

71.594

100

0.716
  pilT Pseudomonas stutzeri DSM 10701

71.594

100

0.716
  pilT Acinetobacter baylyi ADP1

71.594

100

0.716
  pilT Legionella pneumophila strain ERS1305867

71.594

100

0.716
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.68

97.971

0.643
  pilT Vibrio cholerae strain A1552

65.68

97.971

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.103

95.362

0.516
  pilU Pseudomonas stutzeri DSM 10701

42.319

100

0.423
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Acinetobacter baylyi ADP1

40.476

97.391

0.394

Multiple sequence alignment