Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NH8B_RS19450 Genome accession   NC_016002
Coordinates   4199095..4200231 (+) Length   378 a.a.
NCBI ID   WP_008955373.1    Uniprot ID   -
Organism   Pseudogulbenkiania sp. NH8B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4194095..4205231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH8B_RS19420 (NH8B_4000) dksA 4194522..4194932 (-) 411 WP_008955367.1 RNA polymerase-binding protein DksA -
  NH8B_RS19425 (NH8B_4001) - 4195140..4195445 (-) 306 WP_014088764.1 c-type cytochrome -
  NH8B_RS19430 (NH8B_4002) - 4195519..4196100 (-) 582 WP_008955369.1 YggT family protein -
  NH8B_RS19435 (NH8B_4003) proC 4196102..4196905 (-) 804 WP_014088765.1 pyrroline-5-carboxylate reductase -
  NH8B_RS19440 (NH8B_4004) - 4196957..4197652 (-) 696 WP_014088766.1 YggS family pyridoxal phosphate-dependent enzyme -
  NH8B_RS19445 (NH8B_4005) pilT 4197819..4198856 (+) 1038 WP_014088767.1 type IV pilus twitching motility protein PilT Machinery gene
  NH8B_RS19450 (NH8B_4006) pilU 4199095..4200231 (+) 1137 WP_008955373.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NH8B_RS19455 (NH8B_4007) - 4200369..4201583 (-) 1215 WP_014088768.1 EAL and HDOD domain-containing protein -
  NH8B_RS19460 (NH8B_4008) thpR 4201617..4202153 (+) 537 WP_255345166.1 RNA 2',3'-cyclic phosphodiesterase -
  NH8B_RS19465 (NH8B_4009) - 4202397..4203131 (-) 735 WP_040653141.1 amino acid ABC transporter ATP-binding protein -
  NH8B_RS19470 (NH8B_4010) - 4203137..4203811 (-) 675 WP_014088770.1 amino acid ABC transporter permease -
  NH8B_RS19475 (NH8B_4011) - 4203808..4204551 (-) 744 WP_008955378.1 amino acid ABC transporter permease -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42097.36 Da        Isoelectric Point: 5.7949

>NTDB_id=42382 NH8B_RS19450 WP_008955373.1 4199095..4200231(+) (pilU) [Pseudogulbenkiania sp. NH8B]
MERDQASKFIHDLLKHAVGKNASDIFISADFPPAMKIDGKIIPVSPQPLSAQHTKELVRSVMNDRQMEEFESSMEANFAI
SPPGIGRFRVSAFIQQGMAGMVLRKINTEIPTFDQLRLPEVLKDVALIKRGLVIFVGGTGSGKSTSLAALVDWRNSHSQD
HIITIEDPIEYVHPHKKSIVTQREVGVDTDSWDIALKNTLRQAPDVILMGEIRDRETMGYGLQFAETGHLCLATLHANNA
NQALDRILNFFPEERHQQVLMDLSLNMRAIISQRLVPLATGRGRVAAVEVMLNSPLMADLIFKGEIASMKEVIGKSRESG
MQTFDQALFDLYEGGLISYEDALRNADSVNDLRLKIKLYGEGAKDRDLLEGIDHLDII

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=42382 NH8B_RS19450 WP_008955373.1 4199095..4200231(+) (pilU) [Pseudogulbenkiania sp. NH8B]
ATGGAAAGAGACCAGGCCTCGAAGTTCATCCACGACCTGCTCAAGCACGCCGTAGGCAAGAACGCTTCGGACATCTTCAT
TTCGGCCGACTTCCCGCCGGCCATGAAGATCGACGGCAAGATCATCCCGGTGTCGCCGCAGCCACTGTCGGCGCAGCACA
CCAAGGAGCTGGTGCGTTCGGTGATGAACGACCGCCAGATGGAAGAGTTCGAATCCTCGATGGAGGCGAACTTCGCCATC
AGCCCGCCGGGCATCGGCCGCTTCCGCGTCAGCGCCTTCATCCAGCAGGGCATGGCCGGCATGGTGCTGCGCAAGATCAA
TACCGAGATTCCCACCTTCGACCAGCTGCGCCTGCCGGAAGTGCTGAAGGACGTGGCCTTGATCAAGCGCGGCCTGGTGA
TCTTCGTCGGCGGCACCGGCTCGGGCAAGTCCACCTCGCTGGCCGCACTGGTGGACTGGCGCAACAGCCACAGCCAGGAC
CACATCATCACCATCGAGGACCCGATCGAATACGTGCACCCGCACAAGAAATCGATCGTGACCCAGCGCGAGGTCGGGGT
GGACACCGACAGCTGGGACATTGCTCTGAAGAATACCCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGGCTATGGCCTGCAATTCGCCGAAACCGGCCACCTCTGCCTCGCCACCCTACACGCCAACAACGCC
AACCAGGCGCTCGACCGCATCCTGAACTTCTTCCCAGAAGAGCGTCACCAGCAGGTGCTGATGGACCTGTCGCTGAACAT
GCGCGCCATCATCTCGCAGCGGCTGGTTCCGCTCGCCACCGGGCGCGGCCGGGTGGCTGCAGTGGAGGTGATGCTCAACA
GCCCGCTGATGGCGGACCTGATCTTCAAGGGCGAGATCGCCTCGATGAAGGAGGTGATCGGCAAGTCGCGCGAGAGCGGC
ATGCAAACCTTCGACCAGGCGCTGTTCGATCTCTACGAGGGTGGTCTGATCAGCTACGAGGATGCGCTACGCAATGCCGA
CTCGGTCAACGACCTGCGCCTGAAGATCAAGCTGTACGGCGAGGGCGCCAAGGATCGCGACCTGCTCGAGGGCATTGACC
ACCTCGACATCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.324

92.328

0.585
  pilU Acinetobacter baylyi ADP1

57.66

94.974

0.548
  pilU Vibrio cholerae strain A1552

52.21

95.767

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Pseudomonas aeruginosa PAK

43.027

89.153

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.47

87.831

0.373
  pilT Vibrio cholerae strain A1552

42.47

87.831

0.373
  pilT Legionella pneumophila strain Lp02

42.342

88.095

0.373
  pilT Legionella pneumophila strain ERS1305867

42.342

88.095

0.373
  pilT Acinetobacter baylyi ADP1

41.298

89.683

0.37
  pilT Pseudomonas stutzeri DSM 10701

41.543

89.153

0.37
  pilT Acinetobacter nosocomialis M2

41.742

88.095

0.368
  pilT Acinetobacter baumannii D1279779

41.742

88.095

0.368
  pilT Acinetobacter baumannii strain A118

41.742

88.095

0.368
  pilT Neisseria meningitidis 8013

40.588

89.947

0.365

Multiple sequence alignment