Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GYK50_RS11155 Genome accession   NZ_CP048752
Coordinates   2637260..2638315 (+) Length   351 a.a.
NCBI ID   WP_093067571.1    Uniprot ID   -
Organism   Psychrobacter pacificensis strain WSY-P01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2632260..2643315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYK50_RS11140 (GYK50_11140) - 2632598..2634289 (+) 1692 WP_212563947.1 NAD+ synthase -
  GYK50_RS11145 (GYK50_11145) - 2634790..2636064 (+) 1275 WP_186475165.1 hemolysin family protein -
  GYK50_RS11150 (GYK50_11150) - 2636165..2636893 (-) 729 WP_071003702.1 YggS family pyridoxal phosphate-dependent enzyme -
  GYK50_RS11155 (GYK50_11155) pilT 2637260..2638315 (+) 1056 WP_093067571.1 type IV pilus twitching motility protein PilT Machinery gene
  GYK50_RS11160 (GYK50_11160) pilU 2638435..2639556 (+) 1122 WP_212563948.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GYK50_RS11165 (GYK50_11165) fur 2639693..2640127 (-) 435 WP_058024231.1 ferric iron uptake transcriptional regulator -
  GYK50_RS11170 (GYK50_11170) - 2640455..2640874 (+) 420 WP_058024230.1 outer membrane protein assembly factor BamE -
  GYK50_RS11175 (GYK50_11175) - 2640925..2641233 (-) 309 WP_212564561.1 RnfH family protein -
  GYK50_RS11180 (GYK50_11180) - 2641284..2642414 (-) 1131 WP_093067566.1 hypothetical protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39033.93 Da        Isoelectric Point: 7.0439

>NTDB_id=422629 GYK50_RS11155 WP_093067571.1 2637260..2638315(+) (pilT) [Psychrobacter pacificensis strain WSY-P01]
MAEKNTLSIEDLLRFTVKHKASDLHISAGMPAMIRTDGEMTRINMPAMTHQVVHQLIYDIMNDKQRADFEEFFETDFSFE
IPNLARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGDVFKRVSDFKRGVVLVTGPTGSGKSTTLAAMINYINESRKEH
ILTIEDPIEFVHESKKSLINQREVHRDTLGFEPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAA
KTIDRVIDVFPAAEKDMIRAMLSESLQAVISQTLLRRQAGGRVAAHEIMLGTPAIRNLIRENKIAQMYSAIQTGAGEGMI
TLDQTLKNLVGKGTISKDVARPYAKQPEAFL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=422629 GYK50_RS11155 WP_093067571.1 2637260..2638315(+) (pilT) [Psychrobacter pacificensis strain WSY-P01]
ATGGCTGAAAAAAACACCTTAAGTATCGAAGACTTGCTACGTTTTACGGTCAAACATAAAGCATCGGATTTACATATTTC
AGCTGGCATGCCTGCGATGATTCGTACCGATGGTGAGATGACACGCATCAATATGCCTGCGATGACGCACCAAGTGGTTC
ATCAGCTCATTTATGACATTATGAACGACAAGCAGCGCGCTGATTTTGAAGAATTTTTTGAGACAGATTTTTCTTTTGAG
ATTCCAAACTTGGCACGTTTTCGAGTCAATGCATTCAATCAAAATCGCGGCGCAGGCGCGGTATTTCGTACCATCCCTTC
TAAAGTATTGACCATGGAAGACTTAGGGATGGGCGACGTGTTCAAACGTGTCTCAGACTTCAAACGTGGCGTGGTGCTAG
TTACCGGCCCAACTGGTTCGGGTAAATCGACGACGCTAGCGGCCATGATTAATTATATTAACGAAAGTCGTAAAGAGCAT
ATTTTAACAATTGAAGATCCGATTGAATTTGTCCATGAATCGAAAAAAAGCCTGATTAACCAGCGTGAAGTGCACCGCGA
TACCTTAGGTTTTGAGCCTGCATTACGTTCAGCATTGCGAGAAGATCCAGATGTCATCTTGGTTGGTGAGTTACGTGACC
TTGAGACCATTCGTTTGGCACTGACTGCGGCCGAAACAGGACATTTAGTATTCGGCACTCTGCATACTAGCTCAGCCGCT
AAGACGATTGACCGTGTCATCGACGTATTCCCTGCCGCCGAAAAAGATATGATTCGTGCCATGCTATCGGAGTCACTACA
AGCCGTTATTTCACAAACCCTATTGCGTCGTCAAGCTGGTGGACGTGTGGCAGCTCATGAAATCATGCTAGGCACACCCG
CTATCCGTAACTTAATACGTGAAAACAAAATTGCTCAGATGTATTCTGCCATCCAAACGGGTGCAGGCGAAGGCATGATT
ACACTCGATCAAACGCTAAAAAACTTGGTTGGCAAAGGCACCATCAGTAAAGATGTGGCTCGCCCTTATGCCAAGCAACC
TGAGGCATTTTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(17-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.13

98.291

0.778
  pilT Acinetobacter baumannii D1279779

79.13

98.291

0.778
  pilT Acinetobacter baumannii strain A118

79.13

98.291

0.778
  pilT Pseudomonas aeruginosa PAK

77.326

98.006

0.758
  pilT Pseudomonas stutzeri DSM 10701

77.035

98.006

0.755
  pilT Acinetobacter baylyi ADP1

76.163

98.006

0.746
  pilT Legionella pneumophila strain Lp02

71.512

98.006

0.701
  pilT Legionella pneumophila strain ERS1305867

71.512

98.006

0.701
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.085

93.732

0.638
  pilT Vibrio cholerae strain A1552

68.085

93.732

0.638
  pilT Neisseria meningitidis 8013

64.348

98.291

0.632
  pilT Neisseria gonorrhoeae MS11

63.768

98.291

0.627
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

98.291

0.499
  pilU Acinetobacter baylyi ADP1

41.691

97.721

0.407
  pilU Pseudomonas stutzeri DSM 10701

40.708

96.581

0.393
  pilU Vibrio cholerae strain A1552

42.724

92.023

0.393

Multiple sequence alignment