Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GYK50_RS11160 Genome accession   NZ_CP048752
Coordinates   2638435..2639556 (+) Length   373 a.a.
NCBI ID   WP_212563948.1    Uniprot ID   -
Organism   Psychrobacter pacificensis strain WSY-P01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2633435..2644556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYK50_RS11145 (GYK50_11145) - 2634790..2636064 (+) 1275 WP_186475165.1 hemolysin family protein -
  GYK50_RS11150 (GYK50_11150) - 2636165..2636893 (-) 729 WP_071003702.1 YggS family pyridoxal phosphate-dependent enzyme -
  GYK50_RS11155 (GYK50_11155) pilT 2637260..2638315 (+) 1056 WP_093067571.1 type IV pilus twitching motility protein PilT Machinery gene
  GYK50_RS11160 (GYK50_11160) pilU 2638435..2639556 (+) 1122 WP_212563948.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GYK50_RS11165 (GYK50_11165) fur 2639693..2640127 (-) 435 WP_058024231.1 ferric iron uptake transcriptional regulator -
  GYK50_RS11170 (GYK50_11170) - 2640455..2640874 (+) 420 WP_058024230.1 outer membrane protein assembly factor BamE -
  GYK50_RS11175 (GYK50_11175) - 2640925..2641233 (-) 309 WP_212564561.1 RnfH family protein -
  GYK50_RS11180 (GYK50_11180) - 2641284..2642414 (-) 1131 WP_093067566.1 hypothetical protein -
  GYK50_RS11185 (GYK50_11185) - 2642724..2643392 (-) 669 WP_212563949.1 c-type cytochrome -
  GYK50_RS11190 (GYK50_11190) - 2643712..2644047 (-) 336 WP_058024226.1 c-type cytochrome -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41647.94 Da        Isoelectric Point: 7.1147

>NTDB_id=422630 GYK50_RS11160 WP_212563948.1 2638435..2639556(+) (pilU) [Psychrobacter pacificensis strain WSY-P01]
MDIDKLLQLMINKNGSDLFITADVAPSMKVNGKILPVGKTPLTADQTMMLVKGVMTPSQQKEFKETNECQFAISDEAQQA
RFRVSAFIQRDMAGMILRKIENKIPTVEELRLPPALKELAMKKRGIILLVGATGTGKSSTLAAMIGHRNQNSHGHIITIE
DPIEFVHKHRGCIITQREVGIDTHSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFETSRHNQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEVHEIKDVMTRSRDSGMQTFDQ
ALFDLYEQGEITYKEAILHADSPNDLRLKIKLSSRNGKANLDEGADSLSLDIS

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=422630 GYK50_RS11160 WP_212563948.1 2638435..2639556(+) (pilU) [Psychrobacter pacificensis strain WSY-P01]
ATGGACATTGATAAATTATTGCAGTTGATGATTAATAAAAATGGCTCTGACCTTTTTATTACTGCTGATGTAGCGCCTTC
CATGAAAGTAAATGGAAAAATTTTACCTGTCGGTAAAACCCCTTTGACCGCTGATCAGACCATGATGTTAGTCAAAGGAG
TGATGACCCCTAGCCAGCAAAAAGAATTTAAAGAGACGAACGAGTGTCAATTTGCTATATCTGATGAAGCACAGCAAGCA
CGTTTTCGGGTCAGTGCATTCATACAGCGCGATATGGCAGGAATGATTTTACGGAAAATTGAGAATAAGATCCCAACTGT
TGAGGAACTACGCTTGCCGCCAGCGCTCAAAGAACTGGCCATGAAAAAGCGCGGCATTATCTTGCTGGTCGGCGCGACCG
GCACAGGTAAATCCTCTACGCTCGCGGCTATGATTGGACATCGTAACCAAAACTCTCATGGGCATATCATTACCATAGAA
GACCCTATCGAGTTTGTCCATAAACATCGTGGTTGTATCATCACTCAGCGTGAAGTCGGTATCGACACTCATTCATTTGA
AGCAGGACTAAAAAATACGCTGCGCCAAGCACCAGATGTCATCTTGATTGGTGAGATTCGTAACCGTGAGGTCATGAGCT
ACGCTATCCAGTACGCTGAGACAGGGCATCTGGTATTTGCAACCTTGCATGCCAATAATGCCAACCAAGCGATTGACCGT
ATTATTCATTTCTTTGAAACCAGTCGTCATAATCAGCTATTTATGGATTTGTCACTGAACTTGCAGGCCATTATCGCTCA
GCAGCTCATTCCAACCCCTGATGGCAAAGGACGCCGTGCTGCTATTGAGATTTTATTAAACTCACAGTTAATTAGCGACT
ATATCCGTAAAGGTGAGGTGCATGAAATCAAAGACGTCATGACACGCTCTCGAGACAGCGGTATGCAAACCTTTGATCAA
GCGCTTTTTGACTTGTATGAACAAGGAGAAATCACTTACAAAGAAGCCATTTTACATGCTGACTCTCCTAACGATTTACG
CCTAAAAATCAAGCTTAGTAGTAGAAATGGTAAAGCGAATTTGGATGAAGGTGCCGATAGTTTATCGTTAGACATCAGTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(39-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.488

100

0.665
  pilU Acinetobacter baylyi ADP1

66.48

95.979

0.638
  pilU Vibrio cholerae strain A1552

53.561

94.102

0.504
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.53

98.123

0.408
  pilT Legionella pneumophila strain Lp02

40.774

90.08

0.367
  pilT Legionella pneumophila strain ERS1305867

40.774

90.08

0.367
  pilT Acinetobacter nosocomialis M2

39.65

91.957

0.365
  pilT Acinetobacter baumannii strain A118

39.65

91.957

0.365
  pilT Acinetobacter baumannii D1279779

39.65

91.957

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.299

89.812

0.362
  pilT Vibrio cholerae strain A1552

40.299

89.812

0.362
  pilT Pseudomonas aeruginosa PAK

39.017

92.761

0.362

Multiple sequence alignment