Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   G3U99_RS06220 Genome accession   NZ_CP048693
Coordinates   1284507..1285613 (+) Length   368 a.a.
NCBI ID   WP_165579717.1    Uniprot ID   -
Organism   Vibrio coralliilyticus OCN008     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1279507..1290613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3U99_RS06190 (G3U99_06165) - 1279913..1280422 (-) 510 WP_242704145.1 hypothetical protein -
  G3U99_RS06195 (G3U99_06170) yggU 1280985..1281275 (-) 291 WP_021458672.1 DUF167 family protein YggU -
  G3U99_RS06200 (G3U99_06175) - 1281275..1281832 (-) 558 WP_006958264.1 YggT family protein -
  G3U99_RS06205 (G3U99_06180) proC 1281871..1282689 (-) 819 WP_019277259.1 pyrroline-5-carboxylate reductase -
  G3U99_RS06210 (G3U99_06185) - 1282717..1283433 (-) 717 WP_165579502.1 YggS family pyridoxal phosphate-dependent enzyme -
  G3U99_RS06215 (G3U99_06190) pilT 1283458..1284495 (+) 1038 WP_006958261.1 type IV pilus twitching motility protein PilT Machinery gene
  G3U99_RS06220 (G3U99_06195) pilU 1284507..1285613 (+) 1107 WP_165579717.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G3U99_RS06225 (G3U99_06200) ruvX 1285723..1286145 (-) 423 WP_029236265.1 Holliday junction resolvase RuvX -
  G3U99_RS06230 (G3U99_06205) - 1286163..1286726 (-) 564 WP_019277262.1 YqgE/AlgH family protein -
  G3U99_RS06235 (G3U99_06210) gshB 1286783..1287733 (-) 951 WP_019277263.1 glutathione synthase -
  G3U99_RS06240 (G3U99_06215) rsmE 1287747..1288478 (-) 732 WP_031343392.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  G3U99_RS06245 (G3U99_06220) - 1288587..1289291 (-) 705 WP_021458544.1 endonuclease -
  G3U99_RS06250 (G3U99_06225) - 1289371..1289868 (-) 498 WP_021458543.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41207.86 Da        Isoelectric Point: 5.4441

>NTDB_id=422395 G3U99_RS06220 WP_165579717.1 1284507..1285613(+) (pilU) [Vibrio coralliilyticus OCN008]
MDINAYLQGMLTEKASDLYITVGAPILYRVDGELRPHGESLSEVEVLTLLHSTMDEERQNDYSHSREANFAVVRDFGRFR
VSAFYQRELPGAVIRRIETTIPTFDDLKLPETLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNTERSGHILTVEDPI
EFVHEHQKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMAKSQGIGMQTFDQALY
QLVVNDKITEEDALHSADSANDLRIMLKTQRGESFGGGALADVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=422395 G3U99_RS06220 WP_165579717.1 1284507..1285613(+) (pilU) [Vibrio coralliilyticus OCN008]
ATGGATATCAATGCTTATCTGCAAGGCATGCTGACTGAGAAAGCGTCAGACTTATACATCACTGTTGGCGCGCCGATACT
TTATCGTGTTGATGGAGAGCTGAGACCGCATGGAGAGAGCTTATCGGAAGTCGAAGTGCTGACGCTACTCCACAGCACCA
TGGATGAAGAGCGCCAGAATGACTATAGCCACAGTCGAGAAGCGAACTTTGCCGTAGTGCGGGATTTTGGTCGTTTTCGT
GTTTCTGCTTTCTATCAGCGAGAATTACCCGGTGCGGTGATTCGTCGAATCGAAACCACCATACCCACATTTGATGACTT
AAAACTGCCTGAAACTCTGAAAGATCTTTCCATCGCCAAGCGCGGCTTGGTCTTAGTAGTGGGCGCCACAGGCTCCGGTA
AATCCACAACAATGGCAGCGATGACCGGTTATCGAAATACTGAGCGCAGCGGCCATATTCTGACAGTTGAAGACCCGATT
GAGTTTGTCCACGAGCATCAGAAATGCATCGTCACGCAACGTGAGGTTGGCTTAGACACGGAAAGTTATGAAGTTGCACT
GAAGAACTCTTTGCGTCAGGCTCCTGATATGATTTTGATCGGTGAGATCCGCTCGAGAGAGACCATGGAGTACGCGATGA
CATTCGCCGAAACGGGGCATCTGTGTATGGCGACTTTGCACGCTAATAATGCTAACCAAGCTTTGGAAAGGATCCTCCAC
CTTGTACCGAAAGAGCAAAAAGAGCAGTTCCTGTTCGATCTCTCCATGAACCTCAAAGGTGTTATCGGTCAGCAGTTGAT
TCGAGATAAAAACGGCCAAGGTCGTCATGGCGTTTTTGAAATCTTACTCAACAGTCCACGAGTATCGGATCTCATTCGAC
GCGGTGATCTGCATGAACTCAAATCAACCATGGCGAAATCCCAGGGAATTGGTATGCAGACGTTTGATCAGGCGCTTTAC
CAACTGGTGGTGAATGACAAAATTACTGAAGAAGATGCGCTGCACAGTGCAGATTCAGCCAATGATCTGCGTATCATGCT
TAAAACTCAACGAGGTGAAAGCTTCGGTGGTGGTGCGCTGGCAGATGTGAAGATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.609

100

0.826
  pilU Pseudomonas stutzeri DSM 10701

57.429

95.109

0.546
  pilU Acinetobacter baylyi ADP1

52.707

95.38

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.248

92.12

0.408
  pilT Acinetobacter baylyi ADP1

42.229

92.663

0.391
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Acinetobacter baumannii D1279779

42.183

92.12

0.389
  pilT Acinetobacter baumannii strain A118

42.183

92.12

0.389
  pilT Acinetobacter nosocomialis M2

42.183

92.12

0.389
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Pseudomonas stutzeri DSM 10701

40.413

92.12

0.372
  pilT Neisseria meningitidis 8013

39.42

93.75

0.37
  pilT Neisseria gonorrhoeae MS11

39.42

93.75

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.538

88.315

0.367
  pilT Vibrio cholerae strain A1552

41.538

88.315

0.367

Multiple sequence alignment