Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G3U99_RS06215 Genome accession   NZ_CP048693
Coordinates   1283458..1284495 (+) Length   345 a.a.
NCBI ID   WP_006958261.1    Uniprot ID   -
Organism   Vibrio coralliilyticus OCN008     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1278458..1289495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3U99_RS06180 (G3U99_06160) - 1278551..1278772 (+) 222 WP_021458667.1 hypothetical protein -
  G3U99_RS06185 - 1278772..1278930 (+) 159 WP_019274018.1 hypothetical protein -
  G3U99_RS06190 (G3U99_06165) - 1279913..1280422 (-) 510 WP_242704145.1 hypothetical protein -
  G3U99_RS06195 (G3U99_06170) yggU 1280985..1281275 (-) 291 WP_021458672.1 DUF167 family protein YggU -
  G3U99_RS06200 (G3U99_06175) - 1281275..1281832 (-) 558 WP_006958264.1 YggT family protein -
  G3U99_RS06205 (G3U99_06180) proC 1281871..1282689 (-) 819 WP_019277259.1 pyrroline-5-carboxylate reductase -
  G3U99_RS06210 (G3U99_06185) - 1282717..1283433 (-) 717 WP_165579502.1 YggS family pyridoxal phosphate-dependent enzyme -
  G3U99_RS06215 (G3U99_06190) pilT 1283458..1284495 (+) 1038 WP_006958261.1 type IV pilus twitching motility protein PilT Machinery gene
  G3U99_RS06220 (G3U99_06195) pilU 1284507..1285613 (+) 1107 WP_165579717.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G3U99_RS06225 (G3U99_06200) ruvX 1285723..1286145 (-) 423 WP_029236265.1 Holliday junction resolvase RuvX -
  G3U99_RS06230 (G3U99_06205) - 1286163..1286726 (-) 564 WP_019277262.1 YqgE/AlgH family protein -
  G3U99_RS06235 (G3U99_06210) gshB 1286783..1287733 (-) 951 WP_019277263.1 glutathione synthase -
  G3U99_RS06240 (G3U99_06215) rsmE 1287747..1288478 (-) 732 WP_031343392.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  G3U99_RS06245 (G3U99_06220) - 1288587..1289291 (-) 705 WP_021458544.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38121.49 Da        Isoelectric Point: 6.1894

>NTDB_id=422394 G3U99_RS06215 WP_006958261.1 1283458..1284495(+) (pilT) [Vibrio coralliilyticus OCN008]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGVPAFNHSEVHRLVFEIMNDAQRSEFEERLEVDFSFALPDVGR
FRVNAFNQSRGCAAVFRTIPTDIPTLEQLEAPSIFENIANMEKGLILVTGPTGSGKSTTLAAMVDHINRHHNKHILTIED
PIEFVHTNNKCLINQREVHNDTHSFNNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKGMVRSMLSESLRAVIAQKLLKRIGGGRTACHEIMMATPAIRNLIREDKVAQMQSVIQTGAAHGMQTMEQHS
KQLIAQGLVDAEEVSKKIEVEQGLF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=422394 G3U99_RS06215 WP_006958261.1 1283458..1284495(+) (pilT) [Vibrio coralliilyticus OCN008]
ATGGATATCGCTGAATTACTGGATTTTAGTGTAAAGCATAATGCTTCAGATCTACATCTTTCTGCTGGTGTATCTCCCAT
GGTACGCATTGATGGTGAAGTAAGAAAGCTTGGCGTACCAGCGTTCAACCATTCCGAAGTGCATCGTTTGGTGTTTGAAA
TTATGAACGATGCTCAGCGTAGCGAATTTGAAGAACGTCTTGAAGTCGACTTTTCTTTTGCACTTCCGGACGTTGGCCGT
TTTAGGGTCAACGCTTTTAACCAATCTCGTGGTTGTGCGGCCGTATTTCGTACCATTCCAACCGATATCCCGACTTTAGA
GCAGCTAGAAGCGCCGTCGATCTTTGAAAATATCGCCAATATGGAGAAAGGATTAATTTTGGTGACAGGCCCGACGGGCT
CAGGTAAATCGACCACTCTGGCGGCAATGGTGGATCATATTAATCGTCACCACAATAAGCATATCCTGACTATTGAAGAT
CCGATTGAGTTTGTGCACACCAATAACAAATGTCTGATCAACCAGCGTGAAGTTCACAATGATACCCATAGTTTTAATAA
CGCGCTGCGCAGTGCATTGCGTGAAGATCCCGATGTCATTTTAGTTGGTGAGCTACGCGATCAGGAAACGATCAGTCTAG
CGCTGACCGCTGCTGAAACCGGCCATTTGGTGTTTGGTACTTTGCACACCAGTTCGGCGGCAAAAACTATCGACCGTATT
ATTGATGTTTTCCCAGGTAGCGACAAAGGTATGGTACGTTCCATGCTCTCTGAATCTCTGCGTGCGGTGATTGCGCAGAA
ACTACTCAAACGAATTGGCGGCGGGAGAACTGCTTGTCATGAAATTATGATGGCAACGCCTGCGATTCGTAACTTGATCC
GCGAAGACAAAGTCGCGCAAATGCAGTCTGTGATTCAGACTGGTGCGGCGCACGGTATGCAAACCATGGAGCAGCACTCA
AAACAGTTGATTGCTCAAGGTCTGGTTGATGCAGAAGAAGTGTCGAAAAAAATCGAAGTTGAGCAAGGTCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

87.246

100

0.872
  pilT Vibrio cholerae strain A1552

87.246

100

0.872
  pilT Acinetobacter baylyi ADP1

68.713

99.13

0.681
  pilT Acinetobacter baumannii strain A118

69.277

96.232

0.667
  pilT Acinetobacter baumannii D1279779

69.277

96.232

0.667
  pilT Acinetobacter nosocomialis M2

68.976

96.232

0.664
  pilT Pseudomonas stutzeri DSM 10701

66.766

97.681

0.652
  pilT Pseudomonas aeruginosa PAK

65.497

99.13

0.649
  pilT Legionella pneumophila strain Lp02

66.869

95.362

0.638
  pilT Legionella pneumophila strain ERS1305867

66.869

95.362

0.638
  pilT Neisseria meningitidis 8013

64.497

97.971

0.632
  pilT Neisseria gonorrhoeae MS11

63.235

98.551

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.818

95.652

0.496
  pilU Vibrio cholerae strain A1552

42.462

94.203

0.4
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

41.25

92.754

0.383

Multiple sequence alignment