Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G0030_RS03140 Genome accession   NZ_CP048681
Coordinates   655815..656852 (-) Length   345 a.a.
NCBI ID   WP_005179768.1    Uniprot ID   N9MH18
Organism   Acinetobacter indicus strain Q186-3_T     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 650815..661852
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G0030_RS03110 (G0030_03110) - 651578..652033 (+) 456 WP_005179777.1 bacteriohemerythrin -
  G0030_RS03115 (G0030_03115) - 652200..653282 (+) 1083 WP_127800517.1 hypothetical protein -
  G0030_RS03120 (G0030_03120) - 653282..653605 (+) 324 WP_005179774.1 RnfH family protein -
  G0030_RS03125 (G0030_03125) - 653648..654046 (-) 399 WP_005179773.1 outer membrane protein assembly factor BamE -
  G0030_RS03130 (G0030_03130) fur 654159..654596 (+) 438 WP_005179771.1 ferric iron uptake transcriptional regulator -
  G0030_RS03135 (G0030_03135) pilU 654669..655787 (-) 1119 WP_005179770.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G0030_RS03140 (G0030_03140) pilT 655815..656852 (-) 1038 WP_005179768.1 type IV pilus twitching motility protein PilT Machinery gene
  G0030_RS03145 (G0030_03145) - 656976..657662 (+) 687 WP_005179764.1 YggS family pyridoxal phosphate-dependent enzyme -
  G0030_RS03150 (G0030_03150) - 657708..658805 (-) 1098 WP_180182214.1 acyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38237.74 Da        Isoelectric Point: 6.5051

>NTDB_id=422232 G0030_RS03140 WP_005179768.1 655815..656852(-) (pilT) [Acinetobacter indicus strain Q186-3_T]
MDITELLAFSAKNGASDLHLSAGMPPLIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDYEDNLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTIEDLGMGQIFRDICDYPRGLVLVTGPTGSGKSTTLAAMIDYINDNRYDHILTVED
PIEFVHQSKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMSTLDQSL
KALVSRGVISPQTARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=422232 G0030_RS03140 WP_005179768.1 655815..656852(-) (pilT) [Acinetobacter indicus strain Q186-3_T]
ATGGATATTACAGAACTATTGGCCTTTTCGGCAAAAAATGGGGCATCGGATTTACACCTGTCGGCAGGCATGCCGCCACT
GATTCGTGTGGATGGTGAAGTACGCCGCATCAATTTGCCCGCCTTGGAACATAAAGAAGTCCACAAGCTGGTCTATGACA
TCATGAACGACAAACAGCGCCGTGATTATGAAGATAACCTGGAAACCGACTTCTCGTTTGAAGTCCCGGGCGTGGCGCGT
TTCCGTGTTAACGCATTTAACCAGAACCGCGGTGCAGGCGCGGTGTTTCGTACCATTCCCTCTAAAGTGCTGACCATTGA
AGATTTGGGAATGGGTCAAATCTTTAGAGATATCTGTGACTATCCACGTGGCCTGGTGCTGGTCACGGGGCCGACTGGTT
CGGGTAAATCCACCACCCTGGCGGCGATGATTGACTATATCAATGACAATCGCTATGACCACATCCTGACCGTGGAAGAC
CCAATTGAATTTGTGCATCAGTCTAAAAAATGTCTGGTCAACCAGCGTGAAGTGCATCGTGATACCCACGGCTTTAATGA
AGCACTACGTTCGGCGCTGCGTGAAGACCCGGATATTATTCTGGTCGGTGAGATGCGTGACCTGGAAACCATCCGTTTGG
CACTTACCGCGGCAGAAACCGGTCACCTGGTCTTTGGTACCCTGCATACCACCTCGGCGGCAAAAACCATTGACCGTGTG
ATTGACGTCTTTCCGGCCGAAGAAAAAGATATGGTGCGTGCCATGCTGTCTGAATCTTTACAGGCGGTCATTTCACAAAC
CCTGCTCAAGAAAAATGGCGGTGGCCGTGTGGCGGCGCATGAAATCATGATCGGGATTCCGGCCATCCGTAACCTGATTC
GTGAAAACAAGGTTGCACAGATGTACTCGGCCATTCAAACCGGTGCCAACTACGGTATGAGTACGCTGGACCAGAGCCTG
AAAGCCCTGGTCAGCCGTGGCGTGATCAGTCCACAGACCGCGCGTACTGTGGCGAAACAGCCAGAATCGTTCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9MH18

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

94.783

100

0.948
  pilT Acinetobacter baumannii D1279779

94.493

100

0.945
  pilT Acinetobacter baumannii strain A118

94.493

100

0.945
  pilT Acinetobacter baylyi ADP1

86.919

99.71

0.867
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Pseudomonas aeruginosa PAK

83.721

99.71

0.835
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.048

97.391

0.672
  pilT Vibrio cholerae strain A1552

69.048

97.391

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

42.09

97.101

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

37.861

100

0.38

Multiple sequence alignment