Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   G0030_RS03135 Genome accession   NZ_CP048681
Coordinates   654669..655787 (-) Length   372 a.a.
NCBI ID   WP_005179770.1    Uniprot ID   N9M802
Organism   Acinetobacter indicus strain Q186-3_T     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 649669..660787
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G0030_RS03105 (G0030_03105) - 650473..651321 (+) 849 WP_075168090.1 class II glutamine amidotransferase -
  G0030_RS03110 (G0030_03110) - 651578..652033 (+) 456 WP_005179777.1 bacteriohemerythrin -
  G0030_RS03115 (G0030_03115) - 652200..653282 (+) 1083 WP_127800517.1 hypothetical protein -
  G0030_RS03120 (G0030_03120) - 653282..653605 (+) 324 WP_005179774.1 RnfH family protein -
  G0030_RS03125 (G0030_03125) - 653648..654046 (-) 399 WP_005179773.1 outer membrane protein assembly factor BamE -
  G0030_RS03130 (G0030_03130) fur 654159..654596 (+) 438 WP_005179771.1 ferric iron uptake transcriptional regulator -
  G0030_RS03135 (G0030_03135) pilU 654669..655787 (-) 1119 WP_005179770.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G0030_RS03140 (G0030_03140) pilT 655815..656852 (-) 1038 WP_005179768.1 type IV pilus twitching motility protein PilT Machinery gene
  G0030_RS03145 (G0030_03145) - 656976..657662 (+) 687 WP_005179764.1 YggS family pyridoxal phosphate-dependent enzyme -
  G0030_RS03150 (G0030_03150) - 657708..658805 (-) 1098 WP_180182214.1 acyltransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41757.97 Da        Isoelectric Point: 6.6668

>NTDB_id=422231 G0030_RS03135 WP_005179770.1 654669..655787(-) (pilU) [Acinetobacter indicus strain Q186-3_T]
MDFNDLLNLMIQQKASDLFITADVEPSMKINGQIVPVAKSKLTGEIVGQLLNSIMTDKQRKEFADTRECNFAITNRDKSA
RFRVSAFQQRDEPGMVLRRIETKIPTMEELKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHGQLFMDLSLNLKAIVAQQLIPTIDGNSRRAAIELLINSPLVSDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLSEEGGERLMHATSNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=422231 G0030_RS03135 WP_005179770.1 654669..655787(-) (pilU) [Acinetobacter indicus strain Q186-3_T]
ATGGACTTTAATGATTTATTGAATTTAATGATTCAGCAAAAAGCATCGGACCTGTTTATTACGGCCGATGTTGAGCCTTC
CATGAAAATTAACGGCCAGATTGTGCCAGTGGCCAAATCCAAACTGACCGGGGAAATTGTCGGCCAGCTGCTCAACTCGA
TTATGACCGACAAGCAGCGCAAGGAATTTGCCGATACCCGTGAATGTAACTTTGCCATCACCAACCGTGATAAAAGCGCA
CGTTTCCGTGTCAGCGCTTTTCAGCAGCGTGATGAACCGGGCATGGTGCTGCGTCGGATTGAAACCAAAATTCCGACCAT
GGAAGAACTGAAACTGCCACCGATTTTAAAAGATCTGGCCATGACCAAACGTGGCATCATCATCTTTGTCGGGGCAACCG
GTACCGGTAAATCGACCTCTTTGGCGTCAATTATTGGTTATCGTAACCAGAATTCCAAAGGTCATATCATCACCATTGAA
GACCCGATTGAATTTGTGCACGAACACGCCGGCTGTATTATTACCCAGCGTGAAGTAGGCATTGATACCGATTCGTTTGA
AGTGGCACTGAAAAACACCTTGCGTCAGGCCCCAGATGTGATCCTGATTGGTGAGATCCGTTCACGTGAAACCATGGATT
ATGCAATTGCCTTTGCCGAAACCGGTCACCTGGTATTTGCCACCCTGCACGCCAACAACGCCAACCAAGCCATTGACCGG
ATTATCCACTTCTTTGAGGCTGACCGTCATGGCCAGCTGTTTATGGATTTATCACTGAACCTGAAAGCCATTGTGGCACA
GCAGCTGATTCCTACTATTGATGGCAACTCACGTCGTGCCGCGATTGAACTGCTGATTAACTCGCCACTGGTGTCCGATT
TAATCCGTAAAGGCGATGTGCACGAAATTAAAGACCTGATGAAGCGTTCACGTGAGTTGGGTATGCAAACCTTTGACCAG
GCCCTGTATGACCTGTATAAAGCCAAGCAGATCAGCTACAAAGATGCCCTGAAACATGCCGACTCGCCAAACGATTTGCG
TCTGCAAATCAAGCTGTCGGAAGAAGGCGGCGAGCGTTTAATGCATGCCACTTCGAATATTACCTTCGACGGGCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9M802

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

68.464

99.731

0.683
  pilU Vibrio cholerae strain A1552

57.55

94.355

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395
  pilT Legionella pneumophila strain Lp02

41.42

90.86

0.376
  pilT Legionella pneumophila strain ERS1305867

41.42

90.86

0.376
  pilT Pseudomonas aeruginosa PAK

40.462

93.011

0.376
  pilT Acinetobacter baumannii D1279779

40.525

92.204

0.374
  pilT Acinetobacter nosocomialis M2

40.525

92.204

0.374
  pilT Acinetobacter baumannii strain A118

40.525

92.204

0.374
  pilT Acinetobacter baylyi ADP1

39.942

92.204

0.368
  pilT Neisseria gonorrhoeae MS11

40.294

91.398

0.368
  pilT Neisseria meningitidis 8013

40.294

91.398

0.368
  pilT Pseudomonas stutzeri DSM 10701

39.017

93.011

0.363

Multiple sequence alignment