Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G0028_RS03980 Genome accession   NZ_CP048659
Coordinates   823311..824348 (-) Length   345 a.a.
NCBI ID   WP_130074021.1    Uniprot ID   -
Organism   Acinetobacter piscicola strain YH12207_T     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 818311..829348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G0028_RS03950 (G0028_03950) - 819035..819490 (+) 456 WP_130074027.1 bacteriohemerythrin -
  G0028_RS03955 (G0028_03955) - 819645..820724 (+) 1080 WP_174493241.1 hypothetical protein -
  G0028_RS03960 (G0028_03960) - 820724..821059 (+) 336 WP_174493240.1 RnfH family protein -
  G0028_RS03965 (G0028_03965) - 821144..821542 (-) 399 WP_130074024.1 outer membrane protein assembly factor BamE -
  G0028_RS03970 (G0028_03970) fur 821655..822092 (+) 438 WP_130074023.1 ferric iron uptake transcriptional regulator -
  G0028_RS03975 (G0028_03975) pilU 822167..823285 (-) 1119 WP_180046112.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G0028_RS03980 (G0028_03980) pilT 823311..824348 (-) 1038 WP_130074021.1 type IV pilus twitching motility protein PilT Machinery gene
  G0028_RS03985 (G0028_03985) - 824470..825159 (+) 690 WP_180046110.1 YggS family pyridoxal phosphate-dependent enzyme -
  G0028_RS03990 (G0028_03990) - 825317..826111 (+) 795 WP_180046108.1 thioesterase family protein -
  G0028_RS03995 (G0028_03995) - 826252..827007 (+) 756 Protein_774 hypothetical protein -
  G0028_RS04000 (G0028_04000) - 827180..827944 (+) 765 WP_130074017.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38293.82 Da        Isoelectric Point: 6.5051

>NTDB_id=422161 G0028_RS03980 WP_130074021.1 823311..824348(-) (pilT) [Acinetobacter piscicola strain YH12207_T]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPAFEHKDVHRLVYDIMNDKQRRDYEEQLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGVPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGTISAQVARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=422161 G0028_RS03980 WP_130074021.1 823311..824348(-) (pilT) [Acinetobacter piscicola strain YH12207_T]
ATGGACATTACAGAACTACTAGCATTTTCCGTTAAAAATGGTGCATCAGATTTACACTTATCAGCAGGTATGCCACCCAT
GATTCGTGTCGATGGTGAAGTGCGCCGTATTAACTTACCTGCATTTGAGCATAAAGATGTACACCGTCTTGTTTATGACA
TTATGAATGATAAACAACGCCGTGATTATGAAGAACAACTCGAAACTGACTTTTCCTTTGAAGTCCCAGGGGTGGCACGT
TTTCGTGTCAACGCATTTAACCAAAATCGCGGTGCAGGTGCGGTATTTCGTACCATTCCATCTAAAGTTTTGACTATGGA
AGACTTAGGTCTTGGGCAAATCTTTAAAGACATTTGTGATTATCCAAGAGGAATCGTGTTGGTCACAGGTCCTACGGGTT
CAGGTAAATCAACTACCCTCGCTGCGATGCTCGATTATATCAATGACAACCGCTATGATCATATTTTAACGGTAGAAGAC
CCGATCGAGTTTGTACACCAGTCTAAAAAATGCTTGATCAATCAACGTGAAGTCCATCGTGATACACACGGCTTTAATGA
AGCACTACGCTCAGCACTGCGTGAAGACCCTGACATTATCTTGGTCGGTGAGATGCGTGACCTTGAGACCATTCGTTTAG
CCTTAACTGCTGCTGAAACAGGTCACTTGGTCTTTGGTACGCTCCATACCACTTCTGCGGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCTGAAGAAAAAGACATGGTCCGTGCCATGTTATCTGAATCTTTACAAGCCGTGATTTCACAAAC
CCTACTGAAGAAAAATGGCGGTGGGCGTGTGGCTGCACATGAAATTATGATCGGTGTACCTGCGATTCGTAACTTGATCC
GTGAAAATAAAGTCGCACAAATGTATTCTGCGATTCAAACAGGTGCCAACTACGGTATGACCACACTCGACCAAAGTTTA
AAAACATTGGTCTCTAAAGGCACGATCAGCGCACAAGTGGCACGTACTGCGGCAAAACAGCCAGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

96.812

100

0.968
  pilT Acinetobacter baumannii D1279779

96.522

100

0.965
  pilT Acinetobacter baumannii strain A118

96.522

100

0.965
  pilT Acinetobacter baylyi ADP1

87.5

99.71

0.872
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.386

96.232

0.687
  pilT Vibrio cholerae strain A1552

71.386

96.232

0.687
  pilT Neisseria meningitidis 8013

67.536

100

0.675
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.67
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.51
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.101

0.397
  pilU Acinetobacter baylyi ADP1

38.439

100

0.386

Multiple sequence alignment