Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   G0028_RS03975 Genome accession   NZ_CP048659
Coordinates   822167..823285 (-) Length   372 a.a.
NCBI ID   WP_180046112.1    Uniprot ID   -
Organism   Acinetobacter piscicola strain YH12207_T     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 817167..828285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G0028_RS03945 (G0028_03945) - 817879..818701 (+) 823 WP_194088746.1 IS5 family transposase -
  G0028_RS03950 (G0028_03950) - 819035..819490 (+) 456 WP_130074027.1 bacteriohemerythrin -
  G0028_RS03955 (G0028_03955) - 819645..820724 (+) 1080 WP_174493241.1 hypothetical protein -
  G0028_RS03960 (G0028_03960) - 820724..821059 (+) 336 WP_174493240.1 RnfH family protein -
  G0028_RS03965 (G0028_03965) - 821144..821542 (-) 399 WP_130074024.1 outer membrane protein assembly factor BamE -
  G0028_RS03970 (G0028_03970) fur 821655..822092 (+) 438 WP_130074023.1 ferric iron uptake transcriptional regulator -
  G0028_RS03975 (G0028_03975) pilU 822167..823285 (-) 1119 WP_180046112.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G0028_RS03980 (G0028_03980) pilT 823311..824348 (-) 1038 WP_130074021.1 type IV pilus twitching motility protein PilT Machinery gene
  G0028_RS03985 (G0028_03985) - 824470..825159 (+) 690 WP_180046110.1 YggS family pyridoxal phosphate-dependent enzyme -
  G0028_RS03990 (G0028_03990) - 825317..826111 (+) 795 WP_180046108.1 thioesterase family protein -
  G0028_RS03995 (G0028_03995) - 826252..827007 (+) 756 Protein_774 hypothetical protein -
  G0028_RS04000 (G0028_04000) - 827180..827944 (+) 765 WP_130074017.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41783.03 Da        Isoelectric Point: 6.3495

>NTDB_id=422160 G0028_RS03975 WP_180046112.1 822167..823285(-) (pilU) [Acinetobacter piscicola strain YH12207_T]
MDFNDLLNMMIEQKASDLFITADVEPSMKINGQIVPVVKTKLTGELVGQIIHSIMNDKQRKEFAETRECNFAIVNKDKTA
RFRVSAFQQRDQPGMILRRIETTIPTMEDLKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASLIGYRNQNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFESDRHSQLFMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLQIKLDEEGGAQLLNANNKLTFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=422160 G0028_RS03975 WP_180046112.1 822167..823285(-) (pilU) [Acinetobacter piscicola strain YH12207_T]
ATGGATTTTAATGATTTGCTTAATATGATGATTGAGCAAAAGGCATCGGACTTGTTTATTACTGCCGATGTTGAACCATC
AATGAAGATTAATGGTCAAATTGTTCCTGTGGTCAAAACTAAATTGACTGGAGAATTGGTCGGGCAAATTATTCACTCAA
TCATGAATGATAAACAACGTAAAGAATTTGCCGAAACGCGTGAGTGCAACTTTGCCATTGTCAACAAAGACAAAACTGCA
CGTTTTCGTGTCAGTGCATTTCAGCAACGTGATCAACCAGGGATGATTTTACGTCGAATCGAAACCACGATTCCGACCAT
GGAAGATTTAAAGCTACCACCAATCTTAAAAGATCTCGCCATGACCAAGCGTGGCATTATCATTTTTGTAGGAGCAACAG
GTACAGGTAAATCGACCTCTTTGGCATCATTGATCGGTTACCGAAATCAAAACTCTAAAGGTCATATTATTACCATCGAA
GACCCAATCGAGTTCGTACATGAACATGCAGGCTGTATTATCACCCAACGCGAAGTAGGCATTGATACAGACTCTTTTGA
AGTCGCACTGAAAAATACTTTACGTCAAGCACCTGACGTCATTTTGATTGGTGAGATTCGTTCTCGTGAAACCATGGATT
ATGCGATTGCCTTTGCTGAAACAGGTCACTTGGTCTTTGCAACCCTGCATGCCAATAACGCCAACCAAGCCATCGATCGT
ATTATTCACTTCTTTGAATCAGACCGTCATAGTCAATTGTTTATGGACTTGTCCTTAAACCTCAAAGCCATGATTGCGCA
GCAACTCATCCCAACCCCAGATGGCAATTCACGCCGTGCGGCAATTGAGATTCTCATTAACTCGCCATTAATTTCAGATT
TGATCCGTAAAGGTGATGTACATGAAATCAAAGATTTAATGAAACGCTCACGTGAATTGGGTATGCAAACCTTTGACCAA
GCTTTATTTGATCTTTATAAAGCAGGTCAAATTACCTATAAAGATGCGCTAAAACATGCAGACTCACCAAATGACTTACG
TCTACAGATTAAGCTTGATGAAGAAGGTGGTGCACAGTTATTGAATGCCAACAACAAACTAACCTTTGACGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.397

100

0.766
  pilU Pseudomonas stutzeri DSM 10701

68.733

99.731

0.685
  pilU Vibrio cholerae strain A1552

55.616

98.118

0.546
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395
  pilT Legionella pneumophila strain Lp02

42.604

90.86

0.387
  pilT Legionella pneumophila strain ERS1305867

42.604

90.86

0.387
  pilT Acinetobacter nosocomialis M2

41.108

92.204

0.379
  pilT Acinetobacter baumannii D1279779

41.108

92.204

0.379
  pilT Acinetobacter baumannii strain A118

41.108

92.204

0.379
  pilT Pseudomonas aeruginosa PAK

40.462

93.011

0.376
  pilT Acinetobacter baylyi ADP1

40.525

92.204

0.374
  pilT Pseudomonas stutzeri DSM 10701

40.173

93.011

0.374
  pilT Neisseria meningitidis 8013

39.884

93.011

0.371
  pilT Neisseria gonorrhoeae MS11

39.884

93.011

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.39

88.978

0.368
  pilT Vibrio cholerae strain A1552

41.39

88.978

0.368

Multiple sequence alignment