Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACIFE_RS13255 Genome accession   NC_015942
Coordinates   2735220..2736269 (-) Length   349 a.a.
NCBI ID   WP_014030081.1    Uniprot ID   -
Organism   Acidithiobacillus ferrivorans SS3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2730220..2741269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIFE_RS13230 (Acife_2757) - 2730320..2731453 (-) 1134 WP_014030076.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  ACIFE_RS13235 (Acife_2758) purE 2731450..2731947 (-) 498 WP_014030077.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  ACIFE_RS13240 (Acife_2759) trmB 2731989..2732681 (+) 693 WP_014030078.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  ACIFE_RS16945 (Acife_2760) - 2732653..2732823 (+) 171 WP_223306716.1 hypothetical protein -
  ACIFE_RS13245 (Acife_2761) - 2732911..2734098 (-) 1188 WP_237700355.1 glycosyltransferase -
  ACIFE_RS13250 (Acife_2762) pilU 2734080..2735210 (-) 1131 WP_014030080.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACIFE_RS13255 (Acife_2763) pilT 2735220..2736269 (-) 1050 WP_014030081.1 type IV pilus twitching motility protein PilT Machinery gene
  ACIFE_RS13260 (Acife_2764) - 2736371..2737042 (+) 672 WP_014030082.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACIFE_RS13265 (Acife_2765) proC 2737039..2737866 (+) 828 WP_014030083.1 pyrroline-5-carboxylate reductase -
  ACIFE_RS13270 (Acife_2766) - 2737863..2738141 (+) 279 WP_014030084.1 YggT family protein -
  ACIFE_RS13275 (Acife_2767) - 2738166..2738516 (+) 351 WP_014030085.1 DUF167 domain-containing protein -
  ACIFE_RS13280 (Acife_2768) bioB 2738513..2739463 (+) 951 WP_014030086.1 biotin synthase BioB -
  ACIFE_RS13285 (Acife_2769) - 2739505..2740074 (-) 570 WP_014030087.1 acyloxyacyl hydrolase -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38424.96 Da        Isoelectric Point: 6.6649

>NTDB_id=42132 ACIFE_RS13255 WP_014030081.1 2735220..2736269(-) (pilT) [Acidithiobacillus ferrivorans SS3]
MDISELLAFAVKNNASDTHISAGLSPMLRINGDIRPLNVEAQDRKAVHGMIYDIMNDSQRKGYEENLEIDFAYELPGVAR
FRVNAFNQDRGPAAAFRTIPAKVLSLEDLNAPKSFVEIINVPRGLVLVTGPTGSGKSTTLAAMVDHINANRADHIITIED
PIEFLHTPKKCLINQREVGANTHSFENALRSALREDPDIILVGELRDLETMRLALTAAETGHIVFATLHTSSAPKTIDRI
VDSFPGGEKDMVRAMLSESLRAVISQTLLKTADGKGRVAAHEIMIATPAIRNLIRENKVAQMYSVIQTGQNQGMQTLDQC
LADLVRAHKVTREDALRRVQNKDSFMNVA

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=42132 ACIFE_RS13255 WP_014030081.1 2735220..2736269(-) (pilT) [Acidithiobacillus ferrivorans SS3]
ATGGACATTTCAGAACTGCTCGCCTTCGCGGTGAAAAACAATGCTTCGGACACGCATATCTCGGCGGGTTTGTCGCCTAT
GCTGCGGATCAACGGCGATATTCGCCCCCTCAATGTGGAGGCGCAGGACAGAAAGGCCGTACATGGCATGATTTACGACA
TCATGAATGATTCTCAGCGGAAGGGGTATGAGGAAAATCTGGAAATCGATTTTGCCTATGAACTGCCCGGTGTGGCGCGT
TTTCGGGTGAATGCCTTCAACCAGGACCGTGGGCCCGCGGCGGCATTTCGGACGATTCCCGCCAAGGTGCTGAGTCTGGA
AGATCTCAATGCACCCAAGTCTTTTGTAGAGATCATCAACGTACCGCGTGGACTGGTGCTGGTCACCGGGCCGACGGGGT
CAGGCAAATCAACGACGCTGGCAGCGATGGTGGATCATATCAATGCGAACCGCGCTGACCATATTATTACCATTGAAGAT
CCCATCGAATTTTTGCACACTCCCAAGAAATGTCTCATCAACCAGCGTGAGGTAGGCGCCAACACCCACTCCTTTGAAAA
TGCCCTGCGCTCAGCTTTACGTGAAGATCCGGATATTATTCTGGTGGGCGAATTGCGTGACCTGGAGACCATGCGCCTGG
CGCTGACTGCGGCGGAAACGGGCCATATCGTGTTTGCGACGCTGCATACCAGTTCGGCGCCGAAAACTATCGACCGTATT
GTCGATTCCTTCCCTGGGGGGGAAAAGGATATGGTGCGCGCGATGCTGTCAGAATCTCTGCGCGCAGTTATTTCTCAGAC
CCTGCTCAAAACGGCCGATGGCAAAGGTCGAGTAGCCGCGCATGAAATTATGATTGCCACACCGGCCATCCGAAATCTCA
TTCGCGAAAATAAGGTGGCGCAGATGTATTCGGTGATCCAGACCGGACAAAATCAGGGTATGCAAACGCTCGACCAGTGC
CTTGCTGATTTGGTGCGTGCCCACAAAGTAACCCGCGAAGATGCTCTGCGCCGGGTACAGAATAAAGACAGCTTTATGAA
TGTGGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

68.786

99.14

0.682
  pilT Acinetobacter nosocomialis M2

68.786

99.14

0.682
  pilT Acinetobacter baumannii strain A118

68.786

99.14

0.682
  pilT Pseudomonas stutzeri DSM 10701

68.116

98.854

0.673
  pilT Pseudomonas aeruginosa PAK

67.246

98.854

0.665
  pilT Acinetobacter baylyi ADP1

70.769

93.123

0.659
  pilT Neisseria meningitidis 8013

65.797

98.854

0.65
  pilT Legionella pneumophila strain Lp02

65.507

98.854

0.648
  pilT Neisseria gonorrhoeae MS11

65.507

98.854

0.648
  pilT Legionella pneumophila strain ERS1305867

65.507

98.854

0.648
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.923

93.123

0.642
  pilT Vibrio cholerae strain A1552

68.923

93.123

0.642
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.857

100

0.499
  pilU Acinetobacter baylyi ADP1

42.604

96.848

0.413
  pilU Pseudomonas stutzeri DSM 10701

42.262

96.275

0.407
  pilU Vibrio cholerae strain A1552

39.583

96.275

0.381

Multiple sequence alignment