Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACIFE_RS13250 Genome accession   NC_015942
Coordinates   2734080..2735210 (-) Length   376 a.a.
NCBI ID   WP_014030080.1    Uniprot ID   G0JRY3
Organism   Acidithiobacillus ferrivorans SS3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2729080..2740210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIFE_RS16940 (Acife_2755) - 2729588..2729776 (+) 189 WP_148260334.1 hypothetical protein -
  ACIFE_RS13225 (Acife_2756) - 2729773..2730285 (+) 513 WP_014030075.1 disulfide bond formation protein B -
  ACIFE_RS13230 (Acife_2757) - 2730320..2731453 (-) 1134 WP_014030076.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  ACIFE_RS13235 (Acife_2758) purE 2731450..2731947 (-) 498 WP_014030077.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  ACIFE_RS13240 (Acife_2759) trmB 2731989..2732681 (+) 693 WP_014030078.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  ACIFE_RS16945 (Acife_2760) - 2732653..2732823 (+) 171 WP_223306716.1 hypothetical protein -
  ACIFE_RS13245 (Acife_2761) - 2732911..2734098 (-) 1188 WP_237700355.1 glycosyltransferase -
  ACIFE_RS13250 (Acife_2762) pilU 2734080..2735210 (-) 1131 WP_014030080.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACIFE_RS13255 (Acife_2763) pilT 2735220..2736269 (-) 1050 WP_014030081.1 type IV pilus twitching motility protein PilT Machinery gene
  ACIFE_RS13260 (Acife_2764) - 2736371..2737042 (+) 672 WP_014030082.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACIFE_RS13265 (Acife_2765) proC 2737039..2737866 (+) 828 WP_014030083.1 pyrroline-5-carboxylate reductase -
  ACIFE_RS13270 (Acife_2766) - 2737863..2738141 (+) 279 WP_014030084.1 YggT family protein -
  ACIFE_RS13275 (Acife_2767) - 2738166..2738516 (+) 351 WP_014030085.1 DUF167 domain-containing protein -
  ACIFE_RS13280 (Acife_2768) bioB 2738513..2739463 (+) 951 WP_014030086.1 biotin synthase BioB -
  ACIFE_RS13285 (Acife_2769) - 2739505..2740074 (-) 570 WP_014030087.1 acyloxyacyl hydrolase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41568.97 Da        Isoelectric Point: 6.3666

>NTDB_id=42131 ACIFE_RS13250 WP_014030080.1 2734080..2735210(-) (pilU) [Acidithiobacillus ferrivorans SS3]
MSVLDDLLVLMVKKNGSDLYLTVGSPPVMKIDGRAVSLGTEMLKSSQVLNLAKEILGMERLQEFQQEKEINMAISASGIG
RFRVNGFFQRGELSFVLRAIKTQIPSLEQLRLPPILKNLAMASRGLVLFVGATGSGKSTSLASMIQYRNQNAAGHILTIE
DPIEFLHKNAMSIVNQREVGIDTLNYERALENALREAPDVILIGEVRSRDTMDHAMAYAETGHLCMSTLHANNANQAIER
IINFFPEDRKKQLLMDLSLNLRAVVSQRLLPIKGQGGRVAAIEVLINTPAIADLIYKGEVGLLKDAMARTNDVGMQTFDQ
SLRQLFMDGLVEYEDALRGADSQNDLRLAIKQECMRRGVEDPGASTSAEGQWRIQS

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=42131 ACIFE_RS13250 WP_014030080.1 2734080..2735210(-) (pilU) [Acidithiobacillus ferrivorans SS3]
ATGTCTGTACTGGATGATCTGCTGGTCTTGATGGTGAAAAAAAACGGCTCTGATCTTTATCTCACCGTAGGCTCGCCGCC
GGTTATGAAAATTGACGGGCGGGCGGTTTCATTAGGGACTGAGATGCTGAAGTCGAGTCAGGTGTTGAATCTTGCTAAAG
AAATTTTGGGCATGGAGCGCTTGCAGGAATTCCAGCAGGAAAAAGAAATTAACATGGCCATCTCCGCGTCCGGGATTGGG
CGTTTCCGGGTCAATGGCTTTTTTCAGCGCGGTGAACTGAGCTTTGTCTTGCGCGCCATTAAGACGCAAATTCCCAGTCT
GGAGCAGCTCAGGCTGCCGCCCATACTGAAAAATCTGGCCATGGCCTCCCGAGGGCTGGTGCTCTTCGTCGGGGCTACCG
GTTCGGGTAAAAGTACTTCTCTGGCCTCGATGATTCAGTATCGCAATCAGAATGCGGCGGGCCATATTCTCACGATTGAG
GACCCCATTGAATTTCTTCACAAGAATGCGATGTCTATCGTCAATCAGCGCGAAGTGGGTATTGATACCCTGAATTACGA
GCGGGCGCTGGAGAATGCCTTGCGGGAGGCGCCAGATGTTATTCTGATCGGCGAAGTGCGCAGTCGGGATACCATGGATC
ATGCCATGGCGTATGCAGAAACCGGACACTTGTGTATGTCGACCTTGCATGCGAACAATGCGAATCAGGCCATTGAACGT
ATCATCAACTTTTTCCCGGAGGACCGCAAAAAACAATTGTTGATGGATCTGTCGCTCAATTTGCGCGCCGTGGTCTCACA
ACGCCTGTTGCCGATCAAGGGGCAGGGCGGGCGAGTCGCCGCGATAGAGGTGCTGATCAATACCCCGGCCATTGCCGATT
TGATCTATAAGGGTGAAGTGGGCTTGCTGAAAGACGCCATGGCGCGGACGAATGATGTGGGTATGCAGACGTTTGATCAA
AGCCTGCGGCAACTGTTTATGGATGGGCTGGTGGAATATGAGGATGCCCTGCGCGGAGCCGATTCACAGAATGACCTGCG
CCTTGCCATCAAGCAGGAGTGCATGCGACGTGGCGTAGAAGACCCTGGTGCCAGTACTTCTGCAGAAGGGCAGTGGCGCA
TTCAGTCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0JRY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.483

92.553

0.551
  pilU Vibrio cholerae strain A1552

54.213

94.681

0.513
  pilU Acinetobacter baylyi ADP1

54.728

92.819

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.228

92.287

0.399
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.848

87.766

0.394
  pilT Vibrio cholerae strain A1552

44.848

87.766

0.394
  pilT Legionella pneumophila strain ERS1305867

42.388

89.096

0.378
  pilT Legionella pneumophila strain Lp02

42.388

89.096

0.378
  pilT Acinetobacter baylyi ADP1

41.742

88.564

0.37
  pilT Neisseria meningitidis 8013

39.826

91.489

0.364
  pilT Pseudomonas aeruginosa PAK

41.141

88.564

0.364
  pilT Acinetobacter baumannii D1279779

40.356

89.628

0.362
  pilT Acinetobacter baumannii strain A118

40.356

89.628

0.362
  pilT Acinetobacter nosocomialis M2

40.356

89.628

0.362

Multiple sequence alignment