Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GYM75_RS04150 Genome accession   NZ_CP048264
Coordinates   945486..946439 (+) Length   317 a.a.
NCBI ID   WP_220216906.1    Uniprot ID   -
Organism   Gilliamella sp. ESL0441     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 940486..951439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYM75_RS04115 (GYM75_04115) ssb 940600..941109 (-) 510 WP_220216899.1 single-stranded DNA-binding protein Machinery gene
  GYM75_RS04120 (GYM75_04120) corA 941324..942265 (+) 942 WP_220216900.1 magnesium/cobalt transporter CorA -
  GYM75_RS04125 (GYM75_04125) - 942489..943457 (+) 969 WP_220216901.1 glucokinase -
  GYM75_RS04130 (GYM75_04130) - 943470..943718 (+) 249 WP_220216902.1 YcgL domain-containing protein -
  GYM75_RS04135 (GYM75_04135) - 943715..944137 (+) 423 WP_220216903.1 YcgN family cysteine cluster protein -
  GYM75_RS04140 (GYM75_04140) - 944166..944723 (-) 558 WP_220216904.1 YggT family protein -
  GYM75_RS04145 (GYM75_04145) - 944761..945462 (-) 702 WP_220216905.1 YggS family pyridoxal phosphate-dependent enzyme -
  GYM75_RS04150 (GYM75_04150) pilT 945486..946439 (+) 954 WP_220216906.1 type IV pilus twitching motility protein PilT Machinery gene
  GYM75_RS04155 (GYM75_04155) proC 946591..947400 (+) 810 WP_220216907.1 pyrroline-5-carboxylate reductase -
  GYM75_RS04160 (GYM75_04160) - 947717..947926 (+) 210 WP_220216908.1 cold shock domain-containing protein -
  GYM75_RS04165 (GYM75_04165) - 948130..948414 (+) 285 WP_220216909.1 DUF5339 domain-containing protein -
  GYM75_RS04170 (GYM75_04170) - 948920..949405 (-) 486 WP_220216910.1 thermonuclease family protein -
  GYM75_RS04175 (GYM75_04175) fabA 949621..950142 (-) 522 WP_220216911.1 bifunctional 3-hydroxydecanoyl-ACP dehydratase/trans-2-decenoyl-ACP isomerase -

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 35506.80 Da        Isoelectric Point: 8.4835

>NTDB_id=420300 GYM75_RS04150 WP_220216906.1 945486..946439(+) (pilT) [Gilliamella sp. ESL0441]
MSNSILNSLLLISVTQKASDLHLSSGEIPCIRINGQLQFITRDKLIAKELESELLKLLNDSQKNDLLQNKQIDFAYHHEE
IGRFRVNIFYQYRGISAVFRILNHQIPTLKEIGAPKVFNSLIQSDSGIILVTGATGSGKSTTLASLIQFINSTSAKHIIT
LEDPIEFIYQNDKSLIQQRELADFSTVLESILRQDPDIILFGELRNKQSIQAALTAAETGHLVLATLHTCSAIQTINRII
DIFPETSRNSIRTQLSNSLRAVISQKLVTNNNRRKASFEILINTPAVSNLINEGKIKQILSLMQTGQQYGMQIMHQD

Nucleotide


Download         Length: 954 bp        

>NTDB_id=420300 GYM75_RS04150 WP_220216906.1 945486..946439(+) (pilT) [Gilliamella sp. ESL0441]
ATGTCAAATTCAATATTAAATTCATTATTACTTATTAGTGTAACGCAAAAAGCGTCTGATTTGCACCTATCAAGCGGTGA
AATACCTTGTATTCGCATAAATGGACAACTTCAATTTATCACTCGTGATAAATTGATCGCAAAAGAACTTGAAAGTGAAT
TATTAAAACTACTTAATGACTCACAAAAAAACGATTTACTACAAAATAAGCAGATTGATTTTGCTTATCATCATGAAGAA
ATAGGGCGATTTAGGGTAAATATCTTTTATCAATACCGAGGGATTTCAGCTGTTTTTCGTATATTAAATCATCAAATACC
AACTTTAAAAGAGATTGGGGCGCCTAAAGTATTCAATTCATTGATTCAATCTGATAGTGGTATTATTTTAGTGACTGGAG
CCACGGGGAGCGGAAAATCCACTACTTTGGCTTCGTTAATACAATTTATCAATTCAACCTCAGCAAAACATATTATCACA
TTAGAAGATCCAATAGAGTTTATTTATCAAAATGATAAATCATTAATTCAACAACGTGAACTCGCTGATTTTAGTACAGT
GTTAGAAAGTATCTTAAGACAAGATCCCGATATTATATTATTTGGCGAATTACGTAATAAACAATCAATACAAGCTGCAT
TGACCGCTGCCGAAACAGGGCATCTTGTTTTGGCAACCTTACATACTTGTTCAGCTATTCAAACTATTAACCGGATTATT
GATATATTTCCTGAAACTTCAAGAAACTCTATCCGTACTCAATTATCCAACAGTTTAAGGGCAGTAATATCACAAAAGCT
AGTCACCAATAACAATCGGCGCAAAGCCTCGTTCGAAATATTAATTAATACACCAGCAGTGAGCAATTTAATTAATGAAG
GAAAGATCAAACAAATTTTATCATTAATGCAAACCGGTCAACAATATGGCATGCAAATCATGCATCAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

49.527

100

0.495
  pilT Neisseria meningitidis 8013

49.527

100

0.495
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.771

99.054

0.473
  pilT Vibrio cholerae strain A1552

47.771

99.054

0.473
  pilT Acinetobacter baylyi ADP1

47.152

99.685

0.47
  pilT Legionella pneumophila strain ERS1305867

47.284

98.738

0.467
  pilT Legionella pneumophila strain Lp02

47.284

98.738

0.467
  pilT Acinetobacter baumannii D1279779

46.203

99.685

0.461
  pilT Acinetobacter baumannii strain A118

46.203

99.685

0.461
  pilT Acinetobacter nosocomialis M2

46.203

99.685

0.461
  pilT Pseudomonas aeruginosa PAK

45.86

99.054

0.454
  pilT Pseudomonas stutzeri DSM 10701

45.86

99.054

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.433

100

0.42
  pilU Acinetobacter baylyi ADP1

39.623

100

0.397
  pilU Pseudomonas stutzeri DSM 10701

37.261

99.054

0.369
  pilU Vibrio cholerae strain A1552

36.278

100

0.363

Multiple sequence alignment