Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GYM76_RS06070 Genome accession   NZ_CP048263
Coordinates   1333581..1334519 (-) Length   312 a.a.
NCBI ID   WP_255561410.1    Uniprot ID   -
Organism   Gilliamella sp. ESL0443     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1328581..1339519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYM76_RS06040 (GYM76_06050) - 1328697..1330472 (+) 1776 WP_220224891.1 Lon protease family protein -
  GYM76_RS06045 (GYM76_06055) fabA 1330611..1331132 (+) 522 WP_065561925.1 bifunctional 3-hydroxydecanoyl-ACP dehydratase/trans-2-decenoyl-ACP isomerase -
  GYM76_RS06050 (GYM76_06060) - 1331231..1331701 (+) 471 WP_220224892.1 thermonuclease family protein -
  GYM76_RS06055 (GYM76_06065) - 1331721..1332002 (-) 282 WP_220224893.1 DUF5339 domain-containing protein -
  GYM76_RS06060 (GYM76_06070) - 1332160..1332369 (-) 210 WP_034909833.1 cold shock domain-containing protein -
  GYM76_RS06065 (GYM76_06075) proC 1332663..1333472 (-) 810 WP_220224894.1 pyrroline-5-carboxylate reductase -
  GYM76_RS06070 (GYM76_06080) pilT 1333581..1334519 (-) 939 WP_255561410.1 type IV pilus twitching motility protein PilT Machinery gene
  GYM76_RS06075 (GYM76_06085) - 1334556..1335257 (+) 702 WP_220224897.1 YggS family pyridoxal phosphate-dependent enzyme -
  GYM76_RS06080 (GYM76_06090) - 1335294..1335851 (+) 558 WP_220224898.1 YggT family protein -
  GYM76_RS06085 (GYM76_06095) - 1335879..1336301 (-) 423 WP_065561932.1 YcgN family cysteine cluster protein -
  GYM76_RS06090 (GYM76_06100) - 1336298..1336546 (-) 249 WP_081299438.1 YcgL domain-containing protein -
  GYM76_RS06095 (GYM76_06105) - 1336569..1337528 (-) 960 WP_065734559.1 glucokinase -
  GYM76_RS06100 (GYM76_06110) corA 1337716..1338657 (-) 942 WP_065734620.1 magnesium/cobalt transporter CorA -
  GYM76_RS06105 (GYM76_06115) ssb 1338864..1339385 (+) 522 WP_220224899.1 single-stranded DNA-binding protein Machinery gene

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 34964.39 Da        Isoelectric Point: 7.1882

>NTDB_id=420276 GYM76_RS06070 WP_255561410.1 1333581..1334519(-) (pilT) [Gilliamella sp. ESL0443]
MINSMLAFSVTQKASDLHLSSGEKPWIRINGELKPIAQDKLSPQKLEIDLFNLLNDDQKGLLQQHRQIDFGYHLEDIGRF
RVNIFYQKRGISAVFRIIKQKVPSLTDIQAPDVLNELAKAESGIILVTGATGSGKSTTLAALIDHINQTLAKHIITLEDP
IEFIYQSDKSLIQQREINHFGEAIDSLLRQDPDIILLGELRNKQTIQAALTIAETGHLVLATLHTRTAIQTINRIIDVFE
EGTRALIRTQLSNSLKAIICQKLVPYNDSRKAIFEVLINTPAVSNLINEGKTRQILSLMQTGQQFGMQLMID

Nucleotide


Download         Length: 939 bp        

>NTDB_id=420276 GYM76_RS06070 WP_255561410.1 1333581..1334519(-) (pilT) [Gilliamella sp. ESL0443]
ATGATAAATTCAATGTTGGCTTTTAGTGTAACGCAAAAAGCTTCTGATTTGCATCTTTCTAGTGGTGAAAAACCGTGGAT
TCGTATTAATGGTGAGCTAAAACCTATTGCGCAAGATAAATTATCGCCACAAAAACTTGAAATTGATTTATTTAATCTAT
TAAATGACGATCAAAAGGGGCTATTACAACAGCATCGACAAATTGATTTTGGTTATCATCTTGAAGATATAGGTCGTTTT
AGGGTAAATATTTTTTATCAAAAACGTGGTATTTCGGCCGTATTTCGAATTATTAAGCAAAAAGTACCAAGCTTAACTGA
TATTCAGGCACCTGATGTACTAAATGAATTAGCAAAAGCAGAATCTGGAATTATCTTAGTTACTGGCGCAACAGGTAGCG
GTAAATCGACAACTTTAGCTGCATTAATTGATCATATAAATCAGACGTTAGCTAAACATATTATCACATTAGAAGACCCT
ATTGAGTTCATTTATCAAAGTGATAAATCACTTATCCAACAACGCGAAATTAACCATTTTGGTGAAGCAATTGATAGTTT
ATTAAGGCAAGATCCAGATATCATTTTATTGGGTGAATTACGCAATAAACAGACCATTCAAGCTGCATTAACTATTGCTG
AAACCGGTCATTTAGTTCTTGCTACATTGCACACACGTACCGCAATACAGACAATCAACCGTATTATTGATGTCTTTGAA
GAGGGAACACGTGCATTAATTCGCACTCAACTATCAAATAGTTTAAAGGCAATAATATGTCAAAAATTGGTCCCTTATAA
TGATAGCCGAAAAGCCATTTTTGAAGTTCTGATCAACACCCCAGCCGTCAGCAATTTGATCAACGAAGGCAAAACAAGGC
AGATATTATCATTAATGCAAACTGGTCAACAATTTGGTATGCAATTAATGATTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

49.524

100

0.5
  pilT Neisseria meningitidis 8013

49.524

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.452

100

0.478
  pilT Vibrio cholerae strain A1552

47.452

100

0.478
  pilT Legionella pneumophila strain Lp02

47.134

100

0.474
  pilT Legionella pneumophila strain ERS1305867

47.134

100

0.474
  pilT Acinetobacter baumannii D1279779

47.267

99.679

0.471
  pilT Acinetobacter baumannii strain A118

47.267

99.679

0.471
  pilT Acinetobacter nosocomialis M2

47.267

99.679

0.471
  pilT Pseudomonas stutzeri DSM 10701

45.86

100

0.462
  pilT Acinetobacter baylyi ADP1

46.302

99.679

0.462
  pilT Pseudomonas aeruginosa PAK

45.541

100

0.458
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

100

0.433
  pilU Acinetobacter baylyi ADP1

41.72

100

0.42
  pilU Pseudomonas stutzeri DSM 10701

39.677

99.359

0.394
  pilU Vibrio cholerae strain A1552

38.019

100

0.381

Multiple sequence alignment