Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GUY17_RS15310 Genome accession   NZ_CP048031
Coordinates   3587056..3588093 (-) Length   345 a.a.
NCBI ID   WP_059744125.1    Uniprot ID   A0AAE6SVD0
Organism   Shewanella sp. Arc9-LZ     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3582056..3593093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GUY17_RS15290 (GUY17_15315) hemH 3582621..3583634 (-) 1014 WP_162023652.1 ferrochelatase -
  GUY17_RS15295 (GUY17_15320) - 3583885..3584418 (-) 534 WP_101086412.1 glutathione peroxidase -
  GUY17_RS15300 (GUY17_15325) - 3584546..3585649 (-) 1104 WP_162023653.1 hypothetical protein -
  GUY17_RS15305 (GUY17_15330) pilU 3585931..3587043 (-) 1113 WP_162023654.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GUY17_RS15310 (GUY17_15335) pilT 3587056..3588093 (-) 1038 WP_059744125.1 type IV pilus twitching motility protein PilT Machinery gene
  GUY17_RS15315 (GUY17_15340) - 3588121..3588807 (+) 687 WP_162023655.1 YggS family pyridoxal phosphate-dependent enzyme -
  GUY17_RS15320 (GUY17_15345) proC 3589393..3590211 (+) 819 WP_162023656.1 pyrroline-5-carboxylate reductase -
  GUY17_RS15325 (GUY17_15350) - 3590447..3590995 (+) 549 WP_101086407.1 YggT family protein -
  GUY17_RS15330 (GUY17_15355) yggU 3591016..3591315 (+) 300 WP_101086406.1 DUF167 family protein YggU -
  GUY17_RS15335 (GUY17_15360) - 3591429..3591827 (+) 399 WP_254439934.1 DUF4426 domain-containing protein -
  GUY17_RS15340 (GUY17_15365) rdgB 3592193..3592879 (+) 687 WP_174839646.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38371.98 Da        Isoelectric Point: 6.9095

>NTDB_id=419630 GUY17_RS15310 WP_059744125.1 3587056..3588093(-) (pilT) [Shewanella sp. Arc9-LZ]
MEITELLAFSVKHKASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAGAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHKHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPEGEKSMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMSHGMQTLDQCL
QNLVNRGLITREDAMQKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=419630 GUY17_RS15310 WP_059744125.1 3587056..3588093(-) (pilT) [Shewanella sp. Arc9-LZ]
ATGGAAATCACTGAATTACTCGCCTTTAGTGTAAAACACAAAGCATCAGATCTACATCTTTCAGCAGGCGTTTCTCCTAT
GATCCGCGTTGACGGTGAGGTAAGAAAAATTAATTTACCTGCGTTAGATCATCAAGGCGTTCATAGTCTTGTCTATGACA
TCATGAATGATAAACAACGTAAAGACTATGAAGAACATCTAGAAATCGATTTTTCATTCGAAGTGCCTAATCTAGCGCGT
TTTCGTGTTAACGCCTTTAATCAGTCGCGTGGCGCTGGCGCGGTATTCCGTACCATTCCGTCTGACATTTTGTCGCTTGA
GCAACTTGGCGCGCCAGAAATTTTTAAGAAAATTGCCAGTTTCCCACGTGGCTTAGTATTAGTAACCGGGCCTACTGGTT
CGGGTAAATCAACCACATTGGCTGCGATGGTCGATTATGTAAATGAAAACCGACATGACCATATTTTAACCATTGAAGAC
CCTATAGAATTCGTACACCAAAACAAGCAATGCCTAATCAACCAGCGTGAAGTTCACAAACATACACATAGCTTTAATGC
AGCCCTAAGAAGTGCATTACGTGAAGATCCGGATGTTATTCTTGTGGGTGAAATGCGCGACCTCGAAACCATTCGCTTAG
CCATGACCGCCGCTGAAACGGGTCACTTAGTATTCGGTACTTTGCATACCACTTCAGCTGCCAAAACTATTGACCGTATA
GTCGATGTATTCCCTGAAGGTGAAAAGAGCATGGTTCGTACCATGTTGTCAGAGTCACTTCAGGCGGTTATTTCACAAAC
ATTGATCAAGAAAATTGGTGGTGGCCGAGTGGCGGCTCATGAAATCATGATGGGCACCCCAGCGATTCGAAATCTTATTC
GTGAAGATAAAGTCGCACAAATGTATTCAGCTATTCAAACGGGTATGTCTCACGGTATGCAAACCCTTGATCAATGTCTG
CAAAACTTAGTTAATCGTGGGTTAATTACCCGTGAAGATGCGATGCAGAAGAGTTCGAATAAACAAGCTAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.825

99.13

0.791
  pilT Acinetobacter baumannii D1279779

79.825

99.13

0.791
  pilT Acinetobacter baumannii strain A118

79.825

99.13

0.791
  pilT Pseudomonas aeruginosa PAK

78.994

97.971

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.391

100

0.774
  pilT Acinetobacter baylyi ADP1

80.243

95.362

0.765
  pilT Legionella pneumophila strain Lp02

75.367

98.841

0.745
  pilT Legionella pneumophila strain ERS1305867

75.367

98.841

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

69.186

99.71

0.69
  pilT Neisseria gonorrhoeae MS11

68.895

99.71

0.687
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.737

98.261

0.499
  pilU Pseudomonas stutzeri DSM 10701

42.56

97.391

0.414
  pilU Vibrio cholerae strain A1552

41.642

98.841

0.412
  pilU Acinetobacter baylyi ADP1

40.533

97.971

0.397

Multiple sequence alignment