Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GUY17_RS15305 Genome accession   NZ_CP048031
Coordinates   3585931..3587043 (-) Length   370 a.a.
NCBI ID   WP_162023654.1    Uniprot ID   -
Organism   Shewanella sp. Arc9-LZ     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3580931..3592043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GUY17_RS15280 (GUY17_15305) - 3581351..3581911 (-) 561 WP_101086414.1 YqgE/AlgH family protein -
  GUY17_RS15285 (GUY17_15310) yciH 3581945..3582274 (-) 330 WP_059744130.1 stress response translation initiation inhibitor YciH -
  GUY17_RS15290 (GUY17_15315) hemH 3582621..3583634 (-) 1014 WP_162023652.1 ferrochelatase -
  GUY17_RS15295 (GUY17_15320) - 3583885..3584418 (-) 534 WP_101086412.1 glutathione peroxidase -
  GUY17_RS15300 (GUY17_15325) - 3584546..3585649 (-) 1104 WP_162023653.1 hypothetical protein -
  GUY17_RS15305 (GUY17_15330) pilU 3585931..3587043 (-) 1113 WP_162023654.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GUY17_RS15310 (GUY17_15335) pilT 3587056..3588093 (-) 1038 WP_059744125.1 type IV pilus twitching motility protein PilT Machinery gene
  GUY17_RS15315 (GUY17_15340) - 3588121..3588807 (+) 687 WP_162023655.1 YggS family pyridoxal phosphate-dependent enzyme -
  GUY17_RS15320 (GUY17_15345) proC 3589393..3590211 (+) 819 WP_162023656.1 pyrroline-5-carboxylate reductase -
  GUY17_RS15325 (GUY17_15350) - 3590447..3590995 (+) 549 WP_101086407.1 YggT family protein -
  GUY17_RS15330 (GUY17_15355) yggU 3591016..3591315 (+) 300 WP_101086406.1 DUF167 family protein YggU -
  GUY17_RS15335 (GUY17_15360) - 3591429..3591827 (+) 399 WP_254439934.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41077.06 Da        Isoelectric Point: 6.0909

>NTDB_id=419629 GUY17_RS15305 WP_162023654.1 3585931..3587043(-) (pilU) [Shewanella sp. Arc9-LZ]
MDVRPFLNMMVEKKASDLFITAGFPPSAKVDGELRQLSDNRLTPEQSLVFVESLMSDVQKKEFHDTSECNFAFAAKDLGR
FRVSAFWQRESAGCVMRRIETQIPEVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHNHRKSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHNQLLFDLSLNLRGIVAQQLIPKSDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMGKSREQGMQTFDQA
LLDLYVEGEISYADALHHADSPNDLRLMIKLRNNEAASSGSMEGITLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=419629 GUY17_RS15305 WP_162023654.1 3585931..3587043(-) (pilU) [Shewanella sp. Arc9-LZ]
ATGGATGTTCGTCCGTTTTTAAATATGATGGTGGAAAAAAAGGCGTCTGATTTATTTATCACTGCTGGTTTTCCACCCAG
TGCCAAAGTCGACGGAGAGCTACGTCAACTATCCGATAATCGATTAACACCAGAGCAATCATTAGTGTTTGTTGAGTCGT
TGATGAGTGACGTTCAAAAGAAAGAATTTCACGACACTAGTGAGTGTAACTTTGCATTTGCAGCCAAAGATTTAGGTCGT
TTTCGTGTCAGTGCCTTTTGGCAACGCGAATCAGCCGGTTGTGTTATGCGCCGTATCGAAACCCAAATACCTGAAGTTGA
TGACCTAAAACTCCCTCCTATTTTGAAAGACTTGGTCATGAGTAAGCGTGGTCTTATCATTATGGTGGGTGGTACCGGTA
CTGGTAAGTCGACTTCGTTGGCTGCGCTAGTGGGATATCGAAATGCTCATGCTCGCGGACATATTCTGACCATTGAAGAC
CCAGTTGAGTTTGTGCATAACCATCGTAAAAGTATTATTACTCAGCGTGAAGTGGGTATTGATACCGACTCATTTGATGC
CGCGCTTAAAAGCTCTTTGCGTCAAGCGCCAGATGTCATTCTGATTGGTGAAATCCGTACTCAAGAAACCATGGAGTTTG
CGTTGTCATTTGCCGAAACGGGTCATTTATGCATGGCAACCTTGCATGCAAACAACGCTAACCAAGCACTCGATCGCATT
ATGCATTTAGTACCGGAAAGTAAGCACAATCAACTGTTGTTTGATTTGTCGTTAAATTTACGTGGCATTGTAGCGCAACA
GCTTATTCCTAAATCAGACGGCACCGGGCGCCGCGCCGCCATTGAGGTGTTAATTAATACCCCTCGAGTGGCCAGTTTGA
TTGCTAAAAATGAGTTGCATTTACTGAAAGAAACCATGGGTAAGTCACGCGAACAAGGCATGCAGACTTTTGACCAGGCA
TTGCTAGATTTATATGTAGAGGGTGAGATTAGTTACGCCGATGCATTACATCATGCCGACTCGCCAAACGATTTACGTTT
AATGATTAAATTGCGAAATAATGAAGCGGCTTCTTCAGGATCAATGGAAGGCATTACTCTCGACATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.559

100

0.619
  pilU Acinetobacter baylyi ADP1

60.511

95.135

0.576
  pilU Vibrio cholerae strain A1552

57.297

100

0.573
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.166

97.568

0.392
  pilT Legionella pneumophila strain Lp02

41.994

89.459

0.376
  pilT Legionella pneumophila strain ERS1305867

41.994

89.459

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

89.189

0.373
  pilT Vibrio cholerae strain A1552

41.818

89.189

0.373
  pilT Acinetobacter baumannii D1279779

39.766

92.432

0.368
  pilT Acinetobacter baumannii strain A118

39.766

92.432

0.368
  pilT Acinetobacter nosocomialis M2

39.766

92.432

0.368
  pilT Acinetobacter baylyi ADP1

39.474

92.432

0.365
  pilT Pseudomonas aeruginosa PAK

38.841

93.243

0.362

Multiple sequence alignment