Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CFU_RS03905 Genome accession   NC_015856
Coordinates   888553..889596 (-) Length   347 a.a.
NCBI ID   WP_014004744.1    Uniprot ID   G0AI06
Organism   Collimonas fungivorans Ter331     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 883553..894596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFU_RS03885 (CFU_0751) glpK 884508..886004 (+) 1497 WP_041741227.1 glycerol kinase GlpK -
  CFU_RS03890 (CFU_0752) - 886100..886813 (+) 714 WP_041742976.1 MIP/aquaporin family protein -
  CFU_RS03895 (CFU_0753) - 886898..887392 (-) 495 WP_014004742.1 glutathione peroxidase -
  CFU_RS03900 (CFU_0754) pilU 887389..888525 (-) 1137 WP_014004743.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CFU_RS03905 (CFU_0755) pilT 888553..889596 (-) 1044 WP_014004744.1 type IV pilus twitching motility protein PilT Machinery gene
  CFU_RS03910 (CFU_0756) - 889719..890468 (+) 750 WP_041741230.1 YggS family pyridoxal phosphate-dependent enzyme -
  CFU_RS03915 (CFU_0757) proC 890458..891282 (+) 825 WP_041741231.1 pyrroline-5-carboxylate reductase -
  CFU_RS03920 (CFU_0758) - 891408..891731 (-) 324 WP_014004747.1 DUF883 family protein -
  CFU_RS03925 (CFU_0759) ubiA 891802..892656 (-) 855 WP_014004748.1 4-hydroxybenzoate octaprenyltransferase -
  CFU_RS03930 (CFU_0760) - 892768..893715 (-) 948 WP_041742977.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38039.56 Da        Isoelectric Point: 6.6930

>NTDB_id=41939 CFU_RS03905 WP_014004744.1 888553..889596(-) (pilT) [Collimonas fungivorans Ter331]
MDISELLAFSVKNKSSDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHSMIYDIMNDGQRKAYEEHLECDFSFEIPGLAR
FRVNAFNQDRGAAAVLRTIPSTVLTLEQLNAPRIFAELALKPRGLVLVTGPTGSGKSTTLAAMVNHVNENEYAHILTIED
PIEFVHQSNKCLINQREVGPHTHSFSNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPGEEKEMVRAMLSESLQAVISQSLLKTKDGSGRVAAHEIMLGTPAIRNLIRESKIAQMYSAIQTGSNMGMQTLDSN
LTELVKRNVISLASARAAAKTPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=41939 CFU_RS03905 WP_014004744.1 888553..889596(-) (pilT) [Collimonas fungivorans Ter331]
ATGGACATTTCCGAACTTCTGGCTTTTTCGGTGAAGAATAAGTCATCCGATTTGCATTTATCCGCAGGCTTGCCGCCGAT
GATCCGGGTTCATGGCGATGTGCGCCGCATCAACCTGCCGCCGCTGGAGCACAAGGATGTGCACTCCATGATCTATGACA
TCATGAACGACGGCCAGCGCAAAGCGTACGAAGAACATCTGGAATGCGATTTTTCGTTTGAAATCCCCGGCCTGGCGCGC
TTCCGCGTCAATGCCTTCAACCAGGACCGCGGCGCCGCAGCCGTGCTGCGGACGATTCCGTCGACCGTCCTGACCCTGGA
ACAGCTCAACGCGCCGCGCATCTTTGCCGAACTGGCGCTCAAGCCGCGCGGCCTGGTGCTGGTCACCGGCCCGACCGGTT
CCGGCAAATCGACCACGCTGGCGGCCATGGTCAACCATGTCAATGAAAACGAATACGCGCACATCCTGACCATCGAAGAT
CCGATCGAATTCGTGCACCAATCGAACAAGTGCCTGATCAACCAGCGCGAAGTCGGACCGCACACCCATTCTTTCAGCAA
TGCGCTGCGCTCGGCCTTGCGGGAAGATCCTGACGTCATCCTGGTGGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCCGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCCTCCGCCGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCCGGCGAAGAAAAGGAAATGGTGCGCGCCATGTTGTCGGAATCGCTGCAAGCGGTGATTTCGCAAAG
CCTGCTGAAAACCAAGGACGGCTCCGGCCGCGTCGCCGCCCATGAAATCATGCTGGGCACGCCGGCGATCCGCAACCTGA
TCCGCGAAAGCAAGATTGCGCAGATGTATTCGGCGATCCAGACCGGCAGCAACATGGGCATGCAAACCCTAGACAGCAAC
CTGACCGAACTGGTCAAGCGCAATGTGATCTCGCTGGCCAGCGCACGCGCGGCAGCCAAGACACCGGACAACTTCCCGGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0AI06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.812

99.424

0.764
  pilT Acinetobacter baumannii D1279779

76.812

99.424

0.764
  pilT Acinetobacter baumannii strain A118

76.812

99.424

0.764
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

74.783

99.424

0.744
  pilT Legionella pneumophila strain ERS1305867

75.516

97.695

0.738
  pilT Legionella pneumophila strain Lp02

75.516

97.695

0.738
  pilT Acinetobacter baylyi ADP1

73.333

99.424

0.729
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Neisseria gonorrhoeae MS11

68.696

99.424

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.818

95.101

0.683
  pilT Vibrio cholerae strain A1552

71.818

95.101

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.424

0.513
  pilU Pseudomonas stutzeri DSM 10701

40.58

99.424

0.403
  pilU Acinetobacter baylyi ADP1

40.173

99.712

0.401
  pilU Vibrio cholerae strain A1552

39.42

99.424

0.392

Multiple sequence alignment