Detailed information NTG0041938

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CFU_RS03900
• Genome accession: NC_015856
• Coordinates: 887389..888525 (-)
• Length: 378 a.a.
• NCBI ID: WP_014004743.1
• Uniprot ID: G0AI05
• Organism: Collimonas fungivorans Ter331
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 882389..893525

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CFU_RS03880 (CFU_0750)	glpD	882852..884381 (+)	1530	WP_238531398.1	glycerol-3-phosphate dehydrogenase	-
CFU_RS03885 (CFU_0751)	glpK	884508..886004 (+)	1497	WP_041741227.1	glycerol kinase GlpK	-
CFU_RS03890 (CFU_0752)	-	886100..886813 (+)	714	WP_041742976.1	MIP/aquaporin family protein	-
CFU_RS03895 (CFU_0753)	-	886898..887392 (-)	495	WP_014004742.1	glutathione peroxidase	-
CFU_RS03900 (CFU_0754)	pilU	887389..888525 (-)	1137	WP_014004743.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CFU_RS03905 (CFU_0755)	pilT	888553..889596 (-)	1044	WP_014004744.1	type IV pilus twitching motility protein PilT	Machinery gene
CFU_RS03910 (CFU_0756)	-	889719..890468 (+)	750	WP_041741230.1	YggS family pyridoxal phosphate-dependent enzyme	-
CFU_RS03915 (CFU_0757)	proC	890458..891282 (+)	825	WP_041741231.1	pyrroline-5-carboxylate reductase	-
CFU_RS03920 (CFU_0758)	-	891408..891731 (-)	324	WP_014004747.1	DUF883 family protein	-
CFU_RS03925 (CFU_0759)	ubiA	891802..892656 (-)	855	WP_014004748.1	4-hydroxybenzoate octaprenyltransferase	-

Sequence

Protein

• Length: 378 a.a.
• Molecular weight: 42078.46 Da
• Isoelectric Point: 6.4219

>NTDB_id=41938 CFU_RS03900 WP_014004743.1 887389..888525(-) (pilU) [Collimonas fungivorans Ter331]
MERDQATKFMHDLLRLMVSKRGSDLFITADFPPAFKIDGKITPVSNQALTSVHTLELARAIMSDKQAADFEETKECNFAI
SPAGLGRFRASAFIQQGRVGMVLRTITTDIPKFEDLGLPPTLKEVAMTKRGLVIMVGATGSGKSTTLAAMIGYRNENSYG
HIITIEDPVEYIHPHKNCIVTQREVGVDTENWGVALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCLATLHANSS
NQALDRIINFFPEERRPQLLMDLSLNLKGIVSQRLVPLKTVKGRAVAVEILLNSPLISDLIFKGDVHEIKEIMKKSRELG
MQTFDQALFDLYEEDKISYEDALRNADSVNDLRLSIKLRGKDAKDRDLSAGTEHLGIV

Nucleotide

• Length: 1137 bp

>NTDB_id=41938 CFU_RS03900 WP_014004743.1 887389..888525(-) (pilU) [Collimonas fungivorans Ter331]
ATGGAAAGAGATCAAGCCACCAAATTCATGCACGACTTGCTGCGCCTGATGGTCAGCAAGCGCGGCTCCGATTTGTTCAT
TACCGCAGACTTCCCGCCGGCGTTCAAGATCGACGGCAAGATCACGCCGGTATCGAACCAGGCGCTGACTTCGGTCCATA
CTCTCGAACTGGCGCGCGCCATCATGAGCGACAAGCAGGCGGCCGATTTCGAAGAAACCAAGGAATGCAACTTCGCCATC
AGCCCGGCAGGGCTGGGGCGCTTCCGCGCTTCGGCGTTCATCCAGCAAGGCCGGGTCGGCATGGTGCTGCGGACCATCAC
CACCGATATCCCGAAATTCGAAGACCTGGGCTTGCCGCCCACGCTGAAGGAGGTCGCCATGACCAAGCGCGGCCTGGTGA
TCATGGTCGGCGCCACCGGTTCCGGCAAATCGACCACGCTGGCGGCGATGATCGGCTACCGCAACGAAAACAGCTACGGC
CACATCATCACGATTGAAGATCCGGTCGAATACATCCATCCGCACAAGAACTGTATCGTGACCCAGCGCGAAGTCGGCGT
CGACACGGAAAACTGGGGAGTAGCTCTGAAGAACACCTTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCG
ACCGCGAAACCATGGACTACGCCATCGCCTTCGCCGAAACCGGCCACTTGTGCCTGGCCACGCTGCATGCCAACAGCTCC
AACCAGGCGCTCGACCGTATCATCAACTTTTTCCCGGAAGAGCGCCGGCCGCAACTGCTGATGGACTTGTCGCTCAATCT
TAAAGGGATCGTCTCGCAACGCCTGGTGCCGCTGAAAACGGTCAAGGGCCGCGCGGTCGCGGTGGAGATCCTGCTGAATT
CACCTTTGATTTCCGACTTGATCTTCAAGGGCGATGTCCATGAAATCAAGGAAATCATGAAGAAGTCGCGCGAGCTCGGC
ATGCAGACTTTTGACCAGGCCCTGTTCGATTTGTACGAAGAGGATAAAATCAGTTACGAAGACGCTTTGCGCAACGCCGA
CTCGGTCAACGACCTGCGGCTTTCCATCAAGCTGCGCGGCAAGGATGCAAAAGACCGCGACCTGTCTGCCGGCACTGAAC
ACCTGGGGATAGTATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (120-277)

3D structure

• AlphaFold DB: G0AI05

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	66.474	91.534	0.608
pilU	Acinetobacter baylyi ADP1	60.286	92.593	0.558
pilU	Vibrio cholerae strain A1552	57.349	91.799	0.526
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.985	88.624	0.381
pilT	Legionella pneumophila strain Lp02	41.994	87.566	0.368
pilT	Legionella pneumophila strain ERS1305867	41.994	87.566	0.368
pilT	Pseudomonas aeruginosa PAK	41.194	88.624	0.365