Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynMITS9220_RS00095 Genome accession   NZ_CP047958
Coordinates   22217..23278 (-) Length   353 a.a.
NCBI ID   WP_186989865.1    Uniprot ID   A0A7G8ISR0
Organism   Synechococcus sp. MIT S9220     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 17217..28278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynMITS9220_RS00070 (SynMITS9220_00014) - 17407..18081 (+) 675 WP_186989855.1 RNA-binding protein -
  SynMITS9220_RS00075 (SynMITS9220_00015) dusA 18107..19111 (-) 1005 WP_186989857.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynMITS9220_RS00080 (SynMITS9220_00016) msrB 19181..19690 (+) 510 WP_186989859.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynMITS9220_RS00085 (SynMITS9220_00017) - 19620..20948 (+) 1329 WP_186989861.1 NAD(P)/FAD-dependent oxidoreductase -
  SynMITS9220_RS00090 (SynMITS9220_00018) - 20923..22200 (-) 1278 WP_186989863.1 type II secretion system F family protein -
  SynMITS9220_RS00095 (SynMITS9220_00019) pilT 22217..23278 (-) 1062 WP_186989865.1 type IV pilus twitching motility protein PilT Machinery gene
  SynMITS9220_RS00100 (SynMITS9220_00020) pilB/pilB1 23289..25019 (-) 1731 WP_186989867.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  SynMITS9220_RS00105 (SynMITS9220_00021) grpE 25115..25852 (+) 738 WP_186991648.1 nucleotide exchange factor GrpE -
  SynMITS9220_RS00110 (SynMITS9220_00022) dnaJ 25895..27025 (+) 1131 WP_067093422.1 molecular chaperone DnaJ -
  SynMITS9220_RS00115 (SynMITS9220_00023) - 27028..27270 (+) 243 WP_067093421.1 sulfurtransferase TusA family protein -
  SynMITS9220_RS00120 (SynMITS9220_00024) rsgA 27290..28171 (+) 882 WP_186991651.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38791.24 Da        Isoelectric Point: 5.6832

>NTDB_id=418739 SynMITS9220_RS00095 WP_186989865.1 22217..23278(-) (pilT) [Synechococcus sp. MIT S9220]
MELMIEDLMQELVEKGGSDLHIASGQPPFGRFSGQLRAMREEALQEESCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSTIPSIELLNLPPVVVETSKRPRGLVLVTGPTGSGKTTTLAALLDHINHTRAEHILTI
EDPIEFVYKSDRSLVHQRQLNEDTRSFGNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPAQQTQIRVQLSGSLVAVFSQTLCRRSNPEPGQFGRVMAQEIMINTPAIANLIREGKTAQLYSQIQTGGEQGM
QTLENLVSDGDIALQEALAKASKPAELERLVGS

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=418739 SynMITS9220_RS00095 WP_186989865.1 22217..23278(-) (pilT) [Synechococcus sp. MIT S9220]
ATGGAGCTGATGATCGAAGACCTCATGCAGGAACTGGTGGAGAAGGGAGGCAGTGACCTGCATATCGCCAGCGGCCAGCC
CCCCTTTGGACGCTTCAGCGGGCAACTGCGAGCAATGCGTGAGGAAGCTCTACAGGAAGAGAGCTGCAACCGGCTGATCT
TCTCGATGCTCAACAACAGCCAACGCAAGACTTTGGAGCAAACTTGGGAACTCGACTGCGCCTACGGACTCAAAGGAGTG
GCGCGTTTCCGGGTGAATGTTTATCGCCAGAAAGGCAGCTATGCGGCCTGCCTGAGAGCACTGGGCAGCACGATCCCAAG
CATCGAATTGCTGAACCTCCCACCCGTGGTGGTGGAAACCAGCAAGCGACCAAGAGGACTGGTGCTGGTCACCGGACCAA
CAGGCTCAGGCAAGACCACCACCCTGGCGGCGCTGCTCGATCACATCAATCACACCCGTGCCGAACACATCCTCACCATT
GAGGACCCAATCGAATTCGTCTACAAAAGCGACCGAAGCCTGGTGCACCAGCGCCAGCTCAATGAAGACACCCGCAGTTT
TGGCAATGCACTGAGAGCAGCCCTGCGCGAAGACCCGGACGTGATCCTGGTGGGTGAAATGCGCGACTTGGAAACCATTC
AGCTGGCCATCAGTGCCGCGGAGACCGGTCACCTCGTCTTCGGCACTCTGCACACCAGTTCTGCCGCCCAAACCGTGGAC
CGCATGGTGGATGTGTTCCCACCCGCACAGCAAACCCAGATCCGCGTGCAACTGTCCGGAAGTCTGGTGGCGGTCTTCTC
CCAGACGCTCTGTCGTCGCAGCAATCCCGAACCTGGTCAGTTTGGCCGAGTCATGGCGCAGGAAATCATGATCAATACGC
CGGCCATCGCCAACCTGATTCGCGAAGGCAAGACCGCCCAGCTGTACTCACAGATCCAAACCGGCGGTGAACAGGGGATG
CAGACCCTGGAAAATCTCGTGAGCGATGGCGACATCGCTCTACAGGAAGCATTGGCCAAAGCCAGCAAACCTGCTGAGCT
TGAGCGACTTGTCGGCAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8ISR0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.907

97.45

0.516
  pilT Pseudomonas stutzeri DSM 10701

52.907

97.45

0.516
  pilT Acinetobacter baylyi ADP1

51.453

97.45

0.501
  pilT Acinetobacter nosocomialis M2

51.453

97.45

0.501
  pilT Acinetobacter baumannii strain A118

51.163

97.45

0.499
  pilT Acinetobacter baumannii D1279779

51.163

97.45

0.499
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.429

99.15

0.49
  pilT Neisseria meningitidis 8013

50.437

97.167

0.49
  pilT Neisseria gonorrhoeae MS11

50.146

97.167

0.487
  pilT Legionella pneumophila strain ERS1305867

53.125

90.652

0.482
  pilT Legionella pneumophila strain Lp02

53.125

90.652

0.482
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

96.317

0.482
  pilT Vibrio cholerae strain A1552

50

96.317

0.482
  pilU Pseudomonas stutzeri DSM 10701

37.892

99.433

0.377
  pilU Vibrio cholerae strain A1552

39.701

94.901

0.377

Multiple sequence alignment