Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SynMITS9220_RS00100 Genome accession   NZ_CP047958
Coordinates   23289..25019 (-) Length   576 a.a.
NCBI ID   WP_186989867.1    Uniprot ID   -
Organism   Synechococcus sp. MIT S9220     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 18289..30019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynMITS9220_RS00080 (SynMITS9220_00016) msrB 19181..19690 (+) 510 WP_186989859.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynMITS9220_RS00085 (SynMITS9220_00017) - 19620..20948 (+) 1329 WP_186989861.1 NAD(P)/FAD-dependent oxidoreductase -
  SynMITS9220_RS00090 (SynMITS9220_00018) - 20923..22200 (-) 1278 WP_186989863.1 type II secretion system F family protein -
  SynMITS9220_RS00095 (SynMITS9220_00019) pilT 22217..23278 (-) 1062 WP_186989865.1 type IV pilus twitching motility protein PilT Machinery gene
  SynMITS9220_RS00100 (SynMITS9220_00020) pilB/pilB1 23289..25019 (-) 1731 WP_186989867.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  SynMITS9220_RS00105 (SynMITS9220_00021) grpE 25115..25852 (+) 738 WP_186991648.1 nucleotide exchange factor GrpE -
  SynMITS9220_RS00110 (SynMITS9220_00022) dnaJ 25895..27025 (+) 1131 WP_067093422.1 molecular chaperone DnaJ -
  SynMITS9220_RS00115 (SynMITS9220_00023) - 27028..27270 (+) 243 WP_067093421.1 sulfurtransferase TusA family protein -
  SynMITS9220_RS00120 (SynMITS9220_00024) rsgA 27290..28171 (+) 882 WP_186991651.1 ribosome small subunit-dependent GTPase A -
  SynMITS9220_RS00125 (SynMITS9220_00025) - 28146..28487 (-) 342 WP_186989870.1 YbaB/EbfC family nucleoid-associated protein -
  SynMITS9220_RS00130 (SynMITS9220_00026) murB 28512..29444 (-) 933 WP_186989871.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62967.31 Da        Isoelectric Point: 4.7200

>NTDB_id=418740 SynMITS9220_RS00100 WP_186989867.1 23289..25019(-) (pilB/pilB1) [Synechococcus sp. MIT S9220]
MSDDRRHLELELLLQRTIPGELVSADEPSLLQELKAKGFDPVRHAALAERLTELLSKNVGIKDEVPAQEQAITETSQRSN
SAVATNSQTSAPEGSESESVAETATSYLKDFTVGGILEADPDEKAEDSTGPQDLESGLNDAERSPVIDLVDRILMQALEL
DASDIHVEPQQAGLQLRFRQDGVLQSQIETLPSRLVPAVTSRFKIMADLDIAERRVPQDGRIRRRFQNRTVDFRVNSLPS
RYGEKIVLRLLDSSATQLGLDKLITNAEALQLVRTLGSKPFGMILVTGPTGSGKSTTLYSLLAERNEPGINISTVEDPIE
FALPGITQCQVNREKGFDFSQALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDAPSAIARLDEMGVE
PFMVSASLIGIVSQRLLRRVCPQCRIAYRPDPEELGRFGLMASNEADVSFFRANHHEGQADPCPMCQGSGYKGRVGVYEV
LRMNEALATTVAKGATTDMVRQLALEGGMKTLLGYSLDLVREGHTTLEEVGRMVLTDSGLESERRARALSTLTCSGCGGG
LQEGWLECPYCLTPRH

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=418740 SynMITS9220_RS00100 WP_186989867.1 23289..25019(-) (pilB/pilB1) [Synechococcus sp. MIT S9220]
GTGAGCGACGACCGCCGGCATCTGGAACTGGAACTTCTGTTGCAGCGAACCATCCCAGGCGAGCTCGTCAGCGCCGATGA
ACCCTCACTGCTCCAGGAACTGAAAGCGAAGGGGTTTGATCCTGTTCGTCACGCCGCGCTTGCCGAACGCCTGACGGAGC
TGCTCAGCAAGAACGTAGGGATTAAGGATGAAGTTCCAGCCCAAGAACAAGCGATCACAGAAACATCGCAGCGATCCAAC
TCCGCCGTCGCAACCAACAGCCAGACCTCGGCTCCGGAAGGTTCAGAAAGCGAAAGCGTGGCGGAAACAGCCACGTCTTA
CCTGAAGGATTTCACCGTCGGCGGGATTCTGGAAGCCGATCCAGACGAGAAAGCTGAGGACTCCACAGGACCGCAGGATC
TTGAATCAGGCCTCAACGATGCAGAACGCTCCCCCGTGATCGATCTGGTGGATCGCATCCTGATGCAGGCCCTTGAGCTG
GATGCAAGCGACATCCATGTGGAGCCTCAGCAAGCCGGACTTCAACTGCGCTTCCGTCAGGACGGAGTGCTGCAGAGTCA
GATCGAAACCCTTCCGAGCCGACTGGTGCCGGCCGTGACCTCCAGGTTCAAGATCATGGCGGACCTGGACATCGCCGAAC
GGCGCGTGCCCCAGGACGGTCGCATCCGGCGAAGGTTCCAGAACCGCACGGTGGATTTCCGCGTGAACAGCCTGCCGAGC
CGCTACGGGGAAAAGATCGTGCTGCGGCTGCTGGACAGTTCCGCCACTCAACTGGGACTCGACAAGCTGATTACCAATGC
TGAAGCACTTCAGCTCGTGCGCACCCTGGGATCCAAACCCTTCGGGATGATCCTGGTGACAGGGCCGACTGGATCGGGCA
AATCCACCACGCTGTATTCCCTGCTGGCCGAACGCAATGAACCGGGGATCAACATCTCCACAGTTGAGGATCCGATCGAA
TTCGCCCTACCAGGTATTACCCAATGCCAGGTGAACCGGGAAAAAGGCTTCGATTTCAGTCAGGCACTGAGAGCCTTCAT
GCGCCAGGACCCAGACGTGCTGCTGGTCGGCGAAACACGCGATCTGGAAACAGCGAAAACAGCCATCGAAGCTGCTCTCA
CTGGTCACCTGGTGCTGACCACGCTCCACTGCAATGACGCCCCTAGCGCCATCGCCAGACTCGACGAGATGGGTGTGGAG
CCTTTCATGGTCAGCGCCTCACTGATCGGGATCGTGTCCCAGCGTCTACTTCGGCGGGTCTGTCCCCAATGCCGTATTGC
CTACCGGCCAGATCCCGAAGAACTAGGTCGCTTCGGGCTGATGGCAAGCAACGAGGCGGACGTCAGTTTCTTCCGTGCCA
ACCACCATGAAGGCCAGGCCGATCCTTGCCCCATGTGTCAGGGCAGTGGTTACAAAGGGCGAGTCGGGGTCTACGAAGTG
CTGCGCATGAACGAAGCGCTGGCGACCACCGTGGCCAAGGGCGCCACCACCGACATGGTGCGCCAGCTGGCTCTTGAGGG
AGGCATGAAAACACTTCTGGGTTACAGCCTCGATCTAGTGAGAGAGGGGCACACAACCCTGGAAGAAGTGGGCCGCATGG
TGCTCACCGATTCCGGCCTGGAATCAGAGCGGCGGGCCAGAGCCCTGAGCACGCTCACCTGCAGTGGCTGTGGTGGCGGA
CTTCAGGAAGGTTGGCTGGAATGTCCTTACTGCCTGACCCCACGTCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(153-419)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

56.537

98.264

0.556

Multiple sequence alignment