Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynA1825c_RS00095 Genome accession   NZ_CP047957
Coordinates   22306..23382 (-) Length   358 a.a.
NCBI ID   WP_186469762.1    Uniprot ID   A0A7G8IJ67
Organism   Synechococcus sp. A18-25c     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 17306..28382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynA1825c_RS00070 (SynA1825c_00014) - 17439..18035 (+) 597 WP_186470902.1 RNA-binding protein -
  SynA1825c_RS00075 (SynA1825c_00015) dusA 18054..19058 (-) 1005 WP_186469759.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynA1825c_RS00080 (SynA1825c_00016) msrB 19128..19637 (+) 510 WP_186469760.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynA1825c_RS00085 (SynA1825c_00017) - 19672..20862 (+) 1191 WP_370593760.1 NAD(P)/FAD-dependent oxidoreductase -
  SynA1825c_RS00090 (SynA1825c_00018) - 20873..22303 (-) 1431 WP_255478407.1 type II secretion system F family protein -
  SynA1825c_RS00095 (SynA1825c_00019) pilT 22306..23382 (-) 1077 WP_186469762.1 type IV pilus twitching motility protein PilT Machinery gene
  SynA1825c_RS00100 (SynA1825c_00020) pilB/pilB1 23393..25066 (-) 1674 WP_370593811.1 GspE/PulE family protein Machinery gene
  SynA1825c_RS00105 (SynA1825c_00021) grpE 25269..25994 (+) 726 WP_186470904.1 nucleotide exchange factor GrpE -
  SynA1825c_RS00110 (SynA1825c_00022) dnaJ 26037..27167 (+) 1131 WP_186469764.1 molecular chaperone DnaJ -
  SynA1825c_RS00115 (SynA1825c_00023) - 27170..27412 (+) 243 WP_186469765.1 sulfurtransferase TusA family protein -
  SynA1825c_RS00120 (SynA1825c_00024) rsgA 27432..28313 (+) 882 WP_186470905.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39471.16 Da        Isoelectric Point: 6.3946

>NTDB_id=418728 SynA1825c_RS00095 WP_186469762.1 22306..23382(-) (pilT) [Synechococcus sp. A18-25c]
MELMIEDLMQELVEAGGSDLHIASGQPPYGRFSGELRPMRDEPLLEESCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSSIPSIDRLNLPPVVVQTCERPRGLVLVTGPTGSGKTTTLAALLDHINHSRAEHILTI
EDPIEFVYKSDRSLVHQRQLNEDTRSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQTQIRVQLSGSLVAVFSQTLCRRSNPAPGQFGRVMAQEILINTAATANLIREGKTAQLYSQIQTGGELGM
QTLEKALADLVKRKQIAKAEAMAKASKPGELERLIQEP

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=418728 SynA1825c_RS00095 WP_186469762.1 22306..23382(-) (pilT) [Synechococcus sp. A18-25c]
ATGGAGCTGATGATCGAGGACCTGATGCAGGAGCTTGTCGAGGCAGGCGGCAGCGACCTGCACATCGCCAGTGGGCAGCC
CCCCTACGGCCGCTTCAGCGGCGAACTGCGTCCGATGCGGGACGAACCTCTGCTGGAGGAGAGCTGCAACCGGCTGATCT
TCTCCATGCTCAACAACAGTCAGCGCAAGACACTCGAACAGACGTGGGAACTTGACTGCGCCTACGGACTGAAGGGCGTG
GCCCGCTTCCGGGTGAATGTGTACCGTCAGAAGGGAAGCTATGCCGCCTGTCTGAGGGCCCTGGGCAGCAGCATTCCCAG
CATCGATCGGCTGAATCTGCCGCCGGTGGTGGTGCAGACCTGCGAACGACCACGGGGCCTGGTGCTGGTGACAGGACCCA
CGGGCTCGGGAAAGACCACCACCCTGGCGGCGCTGCTCGATCACATCAATCACAGCCGCGCCGAACACATCCTCACCATC
GAGGATCCGATCGAGTTCGTGTACAAGAGCGACCGCAGCCTGGTGCATCAGCGGCAGCTCAATGAAGACACGCGCAGCTT
CGCCAATGCCCTCCGTGCGGCGTTGCGCGAGGACCCGGACGTGATCCTGGTGGGGGAAATGCGGGATCTGGAAACGATTC
AATTGGCGATAAGCGCAGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGTTCGGCCGCGCAGACCGTGGAC
CGCATGGTGGATGTGTTCCCCCCCGAACAGCAGACCCAGATCCGCGTGCAACTCTCCGGAAGCCTGGTGGCTGTGTTCTC
GCAGACCCTCTGTCGCCGCAGCAACCCAGCTCCAGGCCAGTTCGGGCGGGTGATGGCCCAGGAAATCCTGATCAACACCG
CCGCCACAGCCAACCTGATCCGCGAAGGCAAAACCGCACAGCTGTATTCCCAGATCCAGACCGGAGGAGAACTGGGTATG
CAGACCCTGGAGAAAGCTCTGGCTGATCTAGTGAAACGCAAGCAGATCGCCAAGGCGGAAGCCATGGCCAAGGCCTCGAA
ACCCGGGGAATTGGAACGTCTGATCCAAGAGCCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8IJ67

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.035

96.089

0.5
  pilT Pseudomonas stutzeri DSM 10701

51.744

96.089

0.497
  pilT Legionella pneumophila strain Lp02

51.929

94.134

0.489
  pilT Legionella pneumophila strain ERS1305867

51.929

94.134

0.489
  pilT Acinetobacter baumannii D1279779

50.581

96.089

0.486
  pilT Acinetobacter nosocomialis M2

50.581

96.089

0.486
  pilT Acinetobacter baumannii strain A118

50.581

96.089

0.486
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.008

98.603

0.483
  pilT Acinetobacter baylyi ADP1

50

96.089

0.48
  pilT Neisseria meningitidis 8013

49.854

95.81

0.478
  pilT Neisseria gonorrhoeae MS11

49.563

95.81

0.475
  pilT Vibrio cholerae strain A1552

48.529

94.972

0.461
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.529

94.972

0.461
  pilU Pseudomonas stutzeri DSM 10701

38.068

98.324

0.374
  pilU Vibrio cholerae strain A1552

38.235

94.972

0.363
  pilU Acinetobacter baylyi ADP1

37.356

97.207

0.363

Multiple sequence alignment