Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SynA1825c_RS00100 Genome accession   NZ_CP047957
Coordinates   23393..25066 (-) Length   557 a.a.
NCBI ID   WP_370593811.1    Uniprot ID   -
Organism   Synechococcus sp. A18-25c     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 18393..30066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynA1825c_RS00080 (SynA1825c_00016) msrB 19128..19637 (+) 510 WP_186469760.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynA1825c_RS00085 (SynA1825c_00017) - 19672..20862 (+) 1191 WP_370593760.1 NAD(P)/FAD-dependent oxidoreductase -
  SynA1825c_RS00090 (SynA1825c_00018) - 20873..22303 (-) 1431 WP_255478407.1 type II secretion system F family protein -
  SynA1825c_RS00095 (SynA1825c_00019) pilT 22306..23382 (-) 1077 WP_186469762.1 type IV pilus twitching motility protein PilT Machinery gene
  SynA1825c_RS00100 (SynA1825c_00020) pilB/pilB1 23393..25066 (-) 1674 WP_370593811.1 GspE/PulE family protein Machinery gene
  SynA1825c_RS00105 (SynA1825c_00021) grpE 25269..25994 (+) 726 WP_186470904.1 nucleotide exchange factor GrpE -
  SynA1825c_RS00110 (SynA1825c_00022) dnaJ 26037..27167 (+) 1131 WP_186469764.1 molecular chaperone DnaJ -
  SynA1825c_RS00115 (SynA1825c_00023) - 27170..27412 (+) 243 WP_186469765.1 sulfurtransferase TusA family protein -
  SynA1825c_RS00120 (SynA1825c_00024) rsgA 27432..28313 (+) 882 WP_186470905.1 ribosome small subunit-dependent GTPase A -
  SynA1825c_RS00125 (SynA1825c_00025) - 28288..28629 (-) 342 WP_186469766.1 YbaB/EbfC family nucleoid-associated protein -
  SynA1825c_RS00130 (SynA1825c_00026) murB 28654..29586 (-) 933 WP_186469767.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 557 a.a.        Molecular weight: 60526.28 Da        Isoelectric Point: 4.7031

>NTDB_id=418729 SynA1825c_RS00100 WP_370593811.1 23393..25066(-) (pilB/pilB1) [Synechococcus sp. A18-25c]
MGFNPARHAALSEQLTALLDLQSSPHPDHSFGQATPAPTLAINASEPIEPSPDPDAEETTPTVNNMEANTGATNTGANNT
VAETATSYLQEFSVDGVLEQDPEEAAAESAGPEDLESSLNDADRSPVINLVDRILMQALQLGASDIHVEPQQAGLQLRFR
QDGVLQNHIEPLPSRLIPAVTSRFKIMADLDIAERRIAQDGRIRRRFQNRTVDFRVNSLPSRYGEKIVLRLLDSSATQLG
LDKLISNPEALELVRALGSKPFGMILVTGPTGSGKSTTLYSLLAERNEPGINISTVEDPIEYTLPGITQCQVNRDKGFDF
SQALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDAPSAIARLDEMGVEPFMVSASLIGIVSQRLLRR
VCPHCRIAYRPDPEELARFGLMASHEADVSFFKANHQDIEANNCPHCQGSGYKGRVGVYEVLRMNEELAAAVAKGATTDM
VRQLALESGMKTLLGYSLDLVREGHTTLEEVGRMVLTDAGLESERRARALSSLTCSGCGGGLQEGWLECPYCLTPRH

Nucleotide


Download         Length: 1674 bp        

>NTDB_id=418729 SynA1825c_RS00100 WP_370593811.1 23393..25066(-) (pilB/pilB1) [Synechococcus sp. A18-25c]
ATGGGCTTCAATCCAGCGCGGCATGCCGCGCTGAGCGAACAGCTGACGGCACTGTTAGATCTGCAATCCTCCCCTCATCC
AGACCACTCGTTTGGCCAGGCCACCCCTGCACCAACGCTGGCAATCAATGCAAGTGAACCGATCGAGCCTTCACCTGATC
CAGACGCGGAAGAGACCACCCCTACGGTGAACAACATGGAGGCCAACACCGGCGCGACGAACACCGGTGCAAACAACACC
GTGGCGGAGACCGCCACCTCCTATCTACAGGAGTTCTCCGTGGACGGCGTGCTGGAGCAGGACCCGGAGGAGGCAGCAGC
GGAATCAGCCGGTCCGGAGGATCTGGAGTCAAGCCTCAACGATGCGGACCGTTCGCCGGTGATCAACCTGGTGGACCGGA
TCCTGATGCAGGCCCTTCAACTGGGAGCCAGTGACATTCACGTAGAGCCCCAGCAGGCAGGTCTGCAACTGCGCTTCCGT
CAGGACGGTGTGCTGCAAAACCACATTGAACCCCTGCCCAGCCGGCTGATCCCAGCCGTGACCTCCCGCTTCAAAATCAT
GGCGGACCTGGACATCGCCGAGCGCCGCATCGCCCAGGACGGTCGGATCCGCCGTCGTTTCCAGAACCGCACAGTGGACT
TCCGAGTGAACAGCCTGCCCAGCCGGTACGGCGAAAAGATCGTGCTGCGACTACTGGACAGCTCCGCCACCCAGCTGGGT
CTCGACAAGCTGATCAGCAACCCGGAGGCACTGGAATTAGTGCGAGCCCTCGGGTCCAAACCGTTCGGAATGATCCTGGT
GACCGGTCCGACGGGATCCGGTAAATCCACCACTCTCTATTCGTTGCTGGCGGAACGCAACGAGCCAGGAATCAATATCT
CCACGGTTGAAGATCCGATCGAATACACCTTGCCCGGGATCACCCAATGCCAGGTGAACCGAGATAAAGGGTTCGATTTC
AGTCAGGCCCTTCGGGCCTTCATGCGTCAGGACCCGGACGTGCTGCTGGTGGGCGAAACCCGCGATCTGGAAACAGCGAA
AACAGCCATTGAAGCGGCCCTCACCGGTCATTTGGTCTTGACCACCCTGCATTGCAATGACGCCCCCAGCGCGATTGCTC
GTCTGGATGAGATGGGAGTCGAACCATTCATGGTTAGCGCCTCCCTGATCGGGATCGTTTCCCAGCGACTGCTGAGGAGG
GTCTGCCCTCATTGCCGCATCGCCTATCGACCGGATCCTGAAGAATTAGCGCGGTTCGGATTGATGGCCAGCCATGAAGC
TGACGTGAGCTTTTTCAAGGCCAATCACCAAGACATAGAGGCCAACAACTGCCCTCACTGTCAGGGCAGCGGTTACAAGG
GCCGGGTTGGGGTCTACGAGGTGTTGCGCATGAACGAAGAGCTTGCGGCAGCCGTAGCCAAAGGCGCCACCACCGACATG
GTGCGGCAACTGGCTCTGGAAAGCGGCATGAAAACCCTGCTGGGGTACAGCCTCGATCTGGTGCGGGAGGGCCACACCAC
CCTGGAGGAGGTGGGGCGCATGGTGCTCACCGACGCGGGCCTGGAATCGGAACGGCGCGCCCGGGCGCTGAGCTCACTCA
CCTGCAGTGGATGTGGTGGCGGACTCCAGGAAGGTTGGCTGGAATGCCCTTACTGCCTCACACCACGTCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(134-400)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.079

81.508

0.539
  pilB Vibrio cholerae strain A1552

45.982

80.431

0.37

Multiple sequence alignment