Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynMEDNS5_RS00095 Genome accession   NZ_CP047952
Coordinates   21773..22846 (-) Length   357 a.a.
NCBI ID   WP_186585749.1    Uniprot ID   A0A7G8HFE1
Organism   Synechococcus sp. MEDNS5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 16773..27846
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynMEDNS5_RS00070 (SynMEDNS5_00014) - 17035..17628 (+) 594 WP_186583758.1 RNA-binding protein -
  SynMEDNS5_RS00075 (SynMEDNS5_00015) dusA 17647..18651 (-) 1005 WP_186583759.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynMEDNS5_RS00080 (SynMEDNS5_00016) msrB 18712..19230 (+) 519 WP_186583760.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynMEDNS5_RS00085 (SynMEDNS5_00017) - 19265..20464 (+) 1200 WP_370593602.1 NAD(P)/FAD-dependent oxidoreductase -
  SynMEDNS5_RS00090 (SynMEDNS5_00018) - 20500..21756 (-) 1257 WP_186583762.1 type II secretion system F family protein -
  SynMEDNS5_RS00095 (SynMEDNS5_00019) pilT 21773..22846 (-) 1074 WP_186585749.1 type IV pilus twitching motility protein PilT Machinery gene
  SynMEDNS5_RS00100 (SynMEDNS5_00020) pilB/pilB1 22857..24518 (-) 1662 WP_186583763.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  SynMEDNS5_RS00105 (SynMEDNS5_00021) grpE 24614..25336 (+) 723 WP_186583764.1 nucleotide exchange factor GrpE -
  SynMEDNS5_RS00110 (SynMEDNS5_00022) dnaJ 25387..26517 (+) 1131 WP_186583765.1 molecular chaperone DnaJ -
  SynMEDNS5_RS00115 (SynMEDNS5_00023) - 26520..26762 (+) 243 WP_186583766.1 sulfurtransferase TusA family protein -
  SynMEDNS5_RS00120 (SynMEDNS5_00024) rsgA 26782..27663 (+) 882 WP_186585750.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39228.89 Da        Isoelectric Point: 6.6100

>NTDB_id=418697 SynMEDNS5_RS00095 WP_186585749.1 21773..22846(-) (pilT) [Synechococcus sp. MEDNS5]
MEVMIEDLMQELVEAGGSDLHIASGQPPYGRFSGQLRPMRDEPLMEESCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSKIPSIELLNLPPVVVETSKRPRGLVLVTGPTGSGKTTTLAALLDHINHSRAEHILTI
EDPIEFVYKSDQSLVHQRQLNEDTRSFANALRTALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPAQQTQIRVQLSGSLVAVFSQTLCRRRNPAPGQFGRVMAQEIMINTPAIANLIREGKTAQLYSQIQTGGELGM
QTLEKALADLVGRGDITLQEARAKASKPSELDRLAAG

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=418697 SynMEDNS5_RS00095 WP_186585749.1 21773..22846(-) (pilT) [Synechococcus sp. MEDNS5]
ATGGAAGTGATGATCGAAGACCTGATGCAGGAGCTGGTGGAGGCAGGCGGAAGCGACCTGCACATCGCCAGCGGTCAACC
GCCTTACGGTCGCTTCAGCGGCCAGTTGCGGCCCATGCGGGACGAGCCCCTGATGGAGGAAAGCTGCAACCGACTGATCT
TCTCCATGCTCAACAACAGCCAGCGGAAAACGCTCGAGCAGACCTGGGAACTCGACTGTGCGTACGGACTTAAAGGCGTG
GCCCGCTTCCGGGTGAATGTGTATCGCCAGAAAGGCAGCTATGCAGCCTGTCTGCGTGCTCTCGGCAGCAAAATCCCCAG
CATCGAGCTGCTGAATCTGCCCCCTGTGGTGGTGGAAACCAGCAAACGCCCCCGGGGGTTGGTGCTGGTGACCGGCCCGA
CTGGGTCAGGCAAAACCACTACCCTGGCGGCACTGCTGGATCACATCAACCACAGCCGCGCGGAACACATCCTCACGATC
GAGGATCCGATCGAATTCGTTTACAAGAGCGATCAGAGCCTGGTGCATCAACGCCAGCTCAATGAAGACACCCGCAGTTT
CGCCAATGCACTGCGAACGGCCCTGAGAGAGGACCCCGACGTGATCCTAGTGGGAGAAATGCGCGATCTGGAAACCATTC
AGCTGGCGATCAGCGCTGCTGAAACCGGGCACTTGGTGTTCGGCACGTTGCACACCAGCTCAGCGGCCCAGACCGTGGAC
CGCATGGTGGATGTATTCCCCCCCGCTCAACAGACCCAGATCCGCGTGCAGCTCTCGGGGAGCCTGGTGGCCGTGTTCTC
CCAGACCCTGTGCCGCCGCCGCAACCCGGCGCCCGGACAATTCGGCCGGGTGATGGCGCAGGAAATCATGATCAACACAC
CGGCCATCGCCAATCTCATCCGCGAAGGAAAAACCGCCCAGCTGTATTCGCAGATTCAGACCGGTGGCGAACTGGGCATG
CAGACCCTGGAGAAGGCACTGGCGGATCTGGTCGGCCGAGGAGACATCACCCTGCAGGAAGCGCGAGCTAAAGCGAGCAA
GCCATCCGAACTGGATCGGCTCGCGGCAGGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8HFE1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.602

97.199

0.521
  pilT Pseudomonas stutzeri DSM 10701

52.738

97.199

0.513
  pilT Acinetobacter baylyi ADP1

51.437

97.479

0.501
  pilT Acinetobacter baumannii strain A118

51.585

97.199

0.501
  pilT Acinetobacter nosocomialis M2

51.585

97.199

0.501
  pilT Acinetobacter baumannii D1279779

51.585

97.199

0.501
  pilT Legionella pneumophila strain Lp02

51.884

96.639

0.501
  pilT Legionella pneumophila strain ERS1305867

51.884

96.639

0.501
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.573

98.319

0.487
  pilT Neisseria meningitidis 8013

50.44

95.518

0.482
  pilT Neisseria gonorrhoeae MS11

50.147

95.518

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

95.238

0.476
  pilT Vibrio cholerae strain A1552

50

95.238

0.476
  pilU Pseudomonas stutzeri DSM 10701

39.193

97.199

0.381
  pilU Vibrio cholerae strain A1552

38.806

93.838

0.364
  pilU Acinetobacter baylyi ADP1

37.069

97.479

0.361

Multiple sequence alignment