Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SynMEDNS5_RS00100 Genome accession   NZ_CP047952
Coordinates   22857..24518 (-) Length   553 a.a.
NCBI ID   WP_186583763.1    Uniprot ID   -
Organism   Synechococcus sp. MEDNS5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 17857..29518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynMEDNS5_RS00080 (SynMEDNS5_00016) msrB 18712..19230 (+) 519 WP_186583760.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynMEDNS5_RS00085 (SynMEDNS5_00017) - 19265..20464 (+) 1200 WP_370593602.1 NAD(P)/FAD-dependent oxidoreductase -
  SynMEDNS5_RS00090 (SynMEDNS5_00018) - 20500..21756 (-) 1257 WP_186583762.1 type II secretion system F family protein -
  SynMEDNS5_RS00095 (SynMEDNS5_00019) pilT 21773..22846 (-) 1074 WP_186585749.1 type IV pilus twitching motility protein PilT Machinery gene
  SynMEDNS5_RS00100 (SynMEDNS5_00020) pilB/pilB1 22857..24518 (-) 1662 WP_186583763.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  SynMEDNS5_RS00105 (SynMEDNS5_00021) grpE 24614..25336 (+) 723 WP_186583764.1 nucleotide exchange factor GrpE -
  SynMEDNS5_RS00110 (SynMEDNS5_00022) dnaJ 25387..26517 (+) 1131 WP_186583765.1 molecular chaperone DnaJ -
  SynMEDNS5_RS00115 (SynMEDNS5_00023) - 26520..26762 (+) 243 WP_186583766.1 sulfurtransferase TusA family protein -
  SynMEDNS5_RS00120 (SynMEDNS5_00024) rsgA 26782..27663 (+) 882 WP_186585750.1 ribosome small subunit-dependent GTPase A -
  SynMEDNS5_RS00125 (SynMEDNS5_00025) - 27638..27979 (-) 342 WP_186583767.1 YbaB/EbfC family nucleoid-associated protein -
  SynMEDNS5_RS00130 (SynMEDNS5_00026) murB 28010..28942 (-) 933 WP_186583768.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 553 a.a.        Molecular weight: 60511.83 Da        Isoelectric Point: 4.8830

>NTDB_id=418698 SynMEDNS5_RS00100 WP_186583763.1 22857..24518(-) (pilB/pilB1) [Synechococcus sp. MEDNS5]
MSDERRRLELELLLKRTIPGTLSSAEEPDLLNHLTAMGLDPRRHSDLTATLHSLLEPLPAERSPTPQQAPQQVPESIADT
ASSYLAEFSVDGVLENDADEAAMQSSSIVDLEDGLDDADRSPVINLVDRILMQALQLGASDIHVEPQQAGLQLRFRQDGV
LQTSIEPLPSRLVPAVTSRFKIMADLDIAERRVAQDGRIRRRFQNRTVDFRVNCLPSRFGEKIVLRLLDSSATQLGLDKL
ITSETTLDTVRSLGSKPFGMILVTGPTGSGKSTTLYSLLAERNDPGINISTVEDPIEYTLPGITQCQVNREKGFDFSQAL
RAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDAPSAIARLDEMGVEPFMVSASLIGIVSQRLLRRVCPD
CRIPYHPKAEELGRFGLMTSNEEGVTFFKANHREGHSDACPTCQGSGYKGRVGVYEVLSMNEVMAAAVAKGATTDMVRQL
ALESGMKTLLGYSLDLVREGRTTLEEVGRMVLTDAGLESERRARALSTLTCSGCGGGLQEGWLECPYCLTPRH

Nucleotide


Download         Length: 1662 bp        

>NTDB_id=418698 SynMEDNS5_RS00100 WP_186583763.1 22857..24518(-) (pilB/pilB1) [Synechococcus sp. MEDNS5]
GTGAGTGACGAACGCCGCCGACTGGAACTGGAACTGCTGCTGAAACGCACGATCCCCGGGACCCTCAGCAGCGCCGAAGA
ACCGGATCTTCTGAACCATCTCACGGCGATGGGACTGGATCCGAGGCGGCACAGCGACCTGACAGCCACGCTGCACAGCC
TTCTCGAACCATTACCGGCAGAGCGCTCTCCAACACCGCAGCAAGCTCCTCAGCAAGTGCCGGAGAGCATTGCCGACACC
GCCAGCTCCTATCTCGCGGAATTCAGCGTGGACGGGGTACTGGAGAACGACGCCGATGAAGCCGCGATGCAGTCGTCCTC
CATCGTGGATCTGGAAGACGGGCTGGACGATGCCGATCGTTCACCGGTGATCAATCTCGTGGACCGGATCCTGATGCAGG
CGCTGCAGCTGGGAGCTAGCGATATTCACGTGGAGCCCCAGCAGGCTGGATTGCAGTTGCGGTTCCGCCAGGACGGCGTT
CTGCAAACCAGCATCGAACCCCTGCCAAGCCGCTTGGTGCCGGCGGTGACCTCCCGCTTCAAAATCATGGCGGACCTGGA
TATCGCCGAGCGAAGGGTGGCCCAGGACGGACGGATCCGGCGCAGATTCCAGAACCGCACCGTGGACTTTCGGGTGAATT
GCCTGCCCAGTCGCTTCGGGGAGAAGATCGTGCTGCGACTGCTGGATAGCTCCGCCACCCAGCTGGGCCTGGACAAGCTG
ATCACCAGTGAGACAACGCTCGATACCGTGCGATCCCTGGGCTCCAAACCCTTCGGCATGATCCTGGTGACCGGACCCAC
CGGATCGGGGAAATCCACCACGCTCTATTCCTTGCTGGCGGAACGCAACGACCCTGGGATCAACATCTCCACGGTGGAGG
ATCCAATCGAATACACCCTCCCAGGCATCACCCAATGCCAGGTGAACCGGGAGAAGGGGTTCGATTTCAGCCAGGCGCTG
CGGGCCTTCATGCGCCAGGACCCGGATGTGCTGCTCGTGGGCGAGACCCGCGACCTGGAAACGGCCAAAACCGCGATCGA
AGCAGCACTCACCGGTCACCTCGTGCTCACCACGTTGCATTGCAACGACGCTCCCAGTGCCATCGCCCGTCTGGATGAGA
TGGGCGTTGAACCCTTCATGGTGAGCGCGTCACTGATCGGCATCGTCTCCCAGCGACTGCTGCGGCGTGTCTGCCCGGAC
TGCCGCATTCCTTATCACCCGAAAGCCGAGGAACTCGGTCGGTTCGGCTTGATGACCAGCAACGAAGAGGGCGTGACGTT
CTTCAAGGCCAATCACCGCGAGGGGCATTCGGACGCTTGTCCCACCTGTCAGGGCAGCGGCTACAAGGGCCGGGTGGGGG
TATATGAGGTGCTGAGCATGAATGAAGTGATGGCCGCTGCGGTGGCCAAGGGAGCCACCACCGACATGGTGCGCCAGCTG
GCCCTGGAATCAGGGATGAAAACGCTGCTGGGCTACAGCCTTGATCTCGTTCGTGAAGGGCGCACCACCCTGGAGGAAGT
CGGCCGCATGGTGCTCACCGATGCTGGCCTGGAGTCCGAACGCCGGGCCCGGGCCCTGAGCACGCTCACCTGCAGCGGAT
GTGGTGGCGGACTTCAGGAAGGTTGGCTGGAATGTCCGTACTGCCTTACCCCACGCCATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(130-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.004

81.917

0.541

Multiple sequence alignment