Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynA15127_RS00095 Genome accession   NZ_CP047948
Coordinates   21687..22763 (-) Length   358 a.a.
NCBI ID   WP_186485493.1    Uniprot ID   A0A7G8GH44
Organism   Synechococcus sp. A15-127     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 16687..27763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynA15127_RS00070 (SynA15127_00014) - 16971..17618 (+) 648 WP_186484518.1 RNA-binding protein -
  SynA15127_RS00075 (SynA15127_00015) dusA 17626..18630 (-) 1005 WP_186484519.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynA15127_RS00080 (SynA15127_00016) msrB 18696..19199 (+) 504 WP_186484520.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynA15127_RS00085 (SynA15127_00017) - 19234..20439 (+) 1206 WP_370594004.1 NAD(P)/FAD-dependent oxidoreductase -
  SynA15127_RS00090 (SynA15127_00018) - 20414..21664 (-) 1251 WP_186484522.1 type II secretion system F family protein -
  SynA15127_RS00095 (SynA15127_00019) pilT 21687..22763 (-) 1077 WP_186485493.1 type IV pilus twitching motility protein PilT Machinery gene
  SynA15127_RS00100 (SynA15127_00020) pilB/pilB1 22774..24534 (-) 1761 WP_255475520.1 GspE/PulE family protein Machinery gene
  SynA15127_RS00105 (SynA15127_00021) grpE 24698..25378 (+) 681 WP_186484523.1 nucleotide exchange factor GrpE -
  SynA15127_RS00110 (SynA15127_00022) dnaJ 25378..26508 (+) 1131 WP_186484524.1 molecular chaperone DnaJ -
  SynA15127_RS00115 (SynA15127_00023) - 26505..26732 (+) 228 WP_043693985.1 sulfurtransferase TusA family protein -
  SynA15127_RS00120 (SynA15127_00024) rsgA 26739..27638 (+) 900 WP_186484525.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39260.85 Da        Isoelectric Point: 5.2789

>NTDB_id=418657 SynA15127_RS00095 WP_186485493.1 21687..22763(-) (pilT) [Synechococcus sp. A15-127]
MNLMIEELMVQLVEAGGSDLHIATGQPPYGRFSGELRPMLEEPLEEEDCNRLIFSMLNNSQRKTLEQTWELDCAYGLRGV
ARFRVNVYRQKGSYAACLRALGSKIPSIEVLNLPPVVIETSKRPRGLVLVTGPTGSGKTTTLAALLDHINHTRSEHILTI
EDPIEFVYQSDKSLVHQRQLNEDTRSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPGQQTQIRVQLSGSLVAVFSQTLCRRQNPAPGQFGRVMAQEIMINTPATANLIREGKTAQLYSQIQTGGELGM
QTLEKALANMVAEGEISMAEATAKASKPGELERLVGDP

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=418657 SynA15127_RS00095 WP_186485493.1 21687..22763(-) (pilT) [Synechococcus sp. A15-127]
ATGAATCTGATGATCGAAGAACTAATGGTGCAGCTAGTCGAAGCAGGCGGCAGCGATCTGCACATCGCCACCGGGCAGCC
TCCCTACGGACGTTTCAGCGGCGAACTGCGCCCGATGCTGGAGGAACCCCTGGAAGAGGAGGACTGCAACAGGCTGATCT
TCTCGATGCTGAACAACAGCCAGCGCAAGACCCTCGAACAGACCTGGGAACTGGACTGCGCCTACGGACTCAGAGGCGTC
GCCCGTTTCCGGGTGAACGTGTACCGGCAGAAGGGCAGTTATGCCGCCTGCCTGAGGGCACTGGGAAGCAAGATCCCCAG
CATCGAGGTTCTGAACCTGCCTCCGGTCGTTATCGAAACCAGCAAGCGTCCACGAGGCCTGGTGCTGGTCACAGGACCCA
CAGGTTCCGGCAAGACCACCACCCTGGCTGCACTGCTGGATCACATCAATCACACCCGCAGCGAACACATCCTCACCATC
GAGGATCCGATCGAATTCGTATACCAGAGCGATAAAAGCCTGGTGCACCAACGCCAACTCAACGAAGACACCAGGAGCTT
CGCCAATGCCCTGCGTGCGGCATTGCGGGAAGACCCAGATGTGATCCTCGTCGGCGAAATGCGAGACCTGGAAACAATCC
AACTGGCCATCAGCGCTGCTGAAACCGGTCATCTTGTGTTCGGCACGCTGCACACCAGTTCGGCTGCCCAGACGGTGGAT
CGCATGGTGGATGTGTTCCCGCCAGGCCAACAAACGCAGATCCGTGTGCAGCTCTCGGGAAGTCTCGTGGCCGTGTTCTC
CCAGACCCTCTGCCGCCGCCAGAATCCGGCTCCCGGTCAGTTCGGTCGGGTGATGGCACAAGAAATCATGATCAACACCC
CGGCGACGGCCAACCTGATCCGGGAAGGGAAGACTGCTCAGCTCTATTCCCAGATCCAAACCGGTGGGGAACTGGGCATG
CAGACACTTGAAAAAGCGCTGGCGAACATGGTGGCAGAAGGTGAAATCAGCATGGCTGAGGCCACTGCCAAAGCCAGCAA
GCCGGGGGAACTGGAACGTCTGGTCGGTGATCCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8GH44

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.942

96.089

0.528
  pilT Pseudomonas stutzeri DSM 10701

53.779

96.089

0.517
  pilT Acinetobacter baumannii D1279779

52.907

96.089

0.508
  pilT Acinetobacter baumannii strain A118

52.907

96.089

0.508
  pilT Acinetobacter nosocomialis M2

52.616

96.089

0.506
  pilT Legionella pneumophila strain Lp02

53.754

93.017

0.5
  pilT Legionella pneumophila strain ERS1305867

53.754

93.017

0.5
  pilT Acinetobacter baylyi ADP1

51.163

96.089

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.292

98.603

0.486
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.294

94.972

0.478
  pilT Vibrio cholerae strain A1552

50.294

94.972

0.478
  pilT Neisseria gonorrhoeae MS11

49.854

95.81

0.478
  pilT Neisseria meningitidis 8013

50.746

93.575

0.475
  pilU Pseudomonas stutzeri DSM 10701

39.601

98.045

0.388
  pilU Vibrio cholerae strain A1552

39.104

93.575

0.366
  pilU Acinetobacter baylyi ADP1

37.572

96.648

0.363

Multiple sequence alignment