Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynPROS71_RS00095 Genome accession   NZ_CP047945
Coordinates   21982..23055 (-) Length   357 a.a.
NCBI ID   WP_186597769.1    Uniprot ID   A0A7G8FQL5
Organism   Synechococcus sp. PROS-7-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 16982..28055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynPROS71_RS00070 (SynPROS71_00014) - 17076..17675 (+) 600 WP_186595859.1 RNA-binding protein -
  SynPROS71_RS00075 (SynPROS71_00015) dusA 17694..18698 (-) 1005 WP_186595860.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynPROS71_RS00080 (SynPROS71_00016) msrB 18759..19277 (+) 519 WP_186583760.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynPROS71_RS00085 (SynPROS71_00017) - 19312..20511 (+) 1200 WP_370586882.1 NAD(P)/FAD-dependent oxidoreductase -
  SynPROS71_RS00090 (SynPROS71_00018) - 20546..21976 (-) 1431 WP_370586830.1 type II secretion system F family protein -
  SynPROS71_RS00095 (SynPROS71_00019) pilT 21982..23055 (-) 1074 WP_186597769.1 type IV pilus twitching motility protein PilT Machinery gene
  SynPROS71_RS00100 (SynPROS71_00020) pilB/pilB1 23066..24745 (-) 1680 WP_186595862.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  SynPROS71_RS00105 (SynPROS71_00021) grpE 24841..25563 (+) 723 WP_186595863.1 nucleotide exchange factor GrpE -
  SynPROS71_RS00110 (SynPROS71_00022) dnaJ 25614..26744 (+) 1131 WP_186595864.1 molecular chaperone DnaJ -
  SynPROS71_RS00115 (SynPROS71_00023) - 26747..26989 (+) 243 WP_186583766.1 sulfurtransferase TusA family protein -
  SynPROS71_RS00120 (SynPROS71_00024) rsgA 27009..27890 (+) 882 WP_186597771.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39229.97 Da        Isoelectric Point: 6.6098

>NTDB_id=418635 SynPROS71_RS00095 WP_186597769.1 21982..23055(-) (pilT) [Synechococcus sp. PROS-7-1]
MEVMIEDLMQELVEAGGSDLHIASGQPPYGRFSGQLRPMRDEPLMEESCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSKIPSIELLNLPPVVVETSKRPRGLVLVTGPTGSGKTTTLAALLDHINHSRAEHILTI
EDPIEFVYKSDQSLVHQRQLNEDTRSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPAQQTQIRVQLSGSLVAVFSQTLCRRSNPAPGQFGRVMAQEIMINTPAIANLIREGKTAQLYSQIQTGGELGM
QTLEKALADLVKAKQISQPEAMSKASKPGELERLING

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=418635 SynPROS71_RS00095 WP_186597769.1 21982..23055(-) (pilT) [Synechococcus sp. PROS-7-1]
ATGGAAGTGATGATCGAAGACCTGATGCAGGAGCTGGTGGAGGCAGGCGGTAGCGACCTGCACATTGCCAGCGGTCAACC
GCCTTACGGCCGCTTCAGCGGCCAGTTGCGGCCCATGCGGGACGAGCCCTTGATGGAGGAAAGCTGCAACCGGCTGATCT
TCTCCATGCTCAACAACAGCCAGCGCAAAACACTCGAGCAGACCTGGGAGCTCGACTGTGCCTACGGACTCAAAGGGGTG
GCTCGCTTCCGGGTGAATGTGTATCGCCAGAAAGGCAGTTATGCAGCCTGCCTACGGGCTCTCGGCAGCAAAATCCCGAG
CATCGAGCTGCTGAATCTGCCCCCCGTGGTGGTGGAAACCAGCAAACGCCCCCGAGGATTAGTGCTCGTGACCGGCCCGA
CTGGATCAGGCAAAACCACCACCCTGGCGGCCCTACTGGATCACATCAACCACAGCCGCGCGGAACACATTCTCACGATC
GAGGATCCGATCGAATTCGTCTACAAGAGCGATCAGAGCCTGGTGCATCAACGCCAGCTCAATGAAGACACCCGCAGTTT
CGCCAATGCACTGCGAGCAGCCCTCAGGGAAGACCCCGACGTGATCCTGGTGGGAGAAATGCGCGATCTGGAAACCATCC
AGCTGGCGATCAGCGCTGCTGAAACCGGGCACCTGGTGTTCGGCACATTGCACACCAGCTCAGCGGCCCAAACCGTGGAC
CGCATGGTGGATGTCTTCCCCCCCGCTCAGCAAACCCAGATCCGCGTGCAACTCTCGGGGAGCCTGGTGGCGGTGTTCTC
CCAGACCCTGTGCCGCCGCAGCAACCCGGCGCCTGGACAATTCGGCCGGGTGATGGCCCAGGAAATCATGATCAACACAC
CGGCCATCGCCAATCTCATCCGCGAAGGAAAAACCGCCCAGCTGTATTCACAAATTCAAACCGGTGGCGAACTGGGCATG
CAGACCCTGGAGAAGGCCCTGGCCGATCTAGTGAAGGCCAAACAAATTTCGCAACCAGAAGCCATGAGCAAAGCGTCCAA
ACCTGGAGAGCTCGAACGATTAATCAACGGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8FQL5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.779

96.359

0.518
  pilT Pseudomonas stutzeri DSM 10701

53.198

96.359

0.513
  pilT Legionella pneumophila strain Lp02

52.493

95.518

0.501
  pilT Legionella pneumophila strain ERS1305867

52.493

95.518

0.501
  pilT Acinetobacter nosocomialis M2

51.163

96.359

0.493
  pilT Acinetobacter baumannii D1279779

51.163

96.359

0.493
  pilT Acinetobacter baumannii strain A118

51.163

96.359

0.493
  pilT Acinetobacter baylyi ADP1

51.014

96.639

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.864

98.599

0.482
  pilT Neisseria gonorrhoeae MS11

49.422

96.919

0.479
  pilT Neisseria meningitidis 8013

49.422

96.919

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.412

95.238

0.471
  pilT Vibrio cholerae strain A1552

49.412

95.238

0.471
  pilU Pseudomonas stutzeri DSM 10701

38.462

98.319

0.378
  pilU Vibrio cholerae strain A1552

38.348

94.958

0.364
  pilU Acinetobacter baylyi ADP1

37.356

97.479

0.364

Multiple sequence alignment