Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynMVIR181_RS00095 Genome accession   NZ_CP047942
Coordinates   21820..22893 (-) Length   357 a.a.
NCBI ID   WP_186590401.1    Uniprot ID   A0A7G8F0E7
Organism   Synechococcus sp. MVIR-18-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 16820..27893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynMVIR181_RS00070 (SynMVIR181_00015) - 17251..17709 (+) 459 WP_186589550.1 RNA-binding protein -
  SynMVIR181_RS00075 (SynMVIR181_00016) dusA 17728..18732 (-) 1005 WP_186589551.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynMVIR181_RS00080 (SynMVIR181_00017) msrB 18799..19311 (+) 513 WP_186589552.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynMVIR181_RS00085 (SynMVIR181_00018) - 19238..20554 (+) 1317 WP_186589553.1 NAD(P)/FAD-dependent oxidoreductase -
  SynMVIR181_RS00090 (SynMVIR181_00019) - 20529..21803 (-) 1275 WP_186589554.1 type II secretion system F family protein -
  SynMVIR181_RS00095 (SynMVIR181_00020) pilT 21820..22893 (-) 1074 WP_186590401.1 type IV pilus twitching motility protein PilT Machinery gene
  SynMVIR181_RS00100 (SynMVIR181_00021) pilB/pilB1 22905..24677 (-) 1773 WP_255444333.1 GspE/PulE family protein Machinery gene
  SynMVIR181_RS00105 (SynMVIR181_00022) grpE 24676..25482 (+) 807 WP_186589556.1 nucleotide exchange factor GrpE -
  SynMVIR181_RS00110 (SynMVIR181_00023) dnaJ 25525..26655 (+) 1131 WP_186589557.1 molecular chaperone DnaJ -
  SynMVIR181_RS00115 (SynMVIR181_00024) - 26659..26901 (+) 243 WP_186524236.1 sulfurtransferase TusA family protein -
  SynMVIR181_RS00120 (SynMVIR181_00025) rsgA 26888..27802 (+) 915 WP_370593857.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39344.78 Da        Isoelectric Point: 5.6604

>NTDB_id=418603 SynMVIR181_RS00095 WP_186590401.1 21820..22893(-) (pilT) [Synechococcus sp. MVIR-18-1]
MERMIEDLMEQLVNGGGSDLHIASGQPPYGRFSGELRPMQEEPLSEEGCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSKIPSVELLNLPPVVVETSKRPRGLVLVTGPTGSGKTTTLAALLDHINHTRAEHILTI
EDPIEFVYTSDKSLVHQRQLNEDTRSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFETLHTSSAAQTVD
RMVDVFPPGQQTQIRVQLSGSLTAVFSQTLCKRQNPAPGQFGRVMAQEIMINTPAIANLIREGKTAQLYSQIQTGGERGM
QTLERALADLIDQGEISLDEGLSKASKPAELDRLMNH

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=418603 SynMVIR181_RS00095 WP_186590401.1 21820..22893(-) (pilT) [Synechococcus sp. MVIR-18-1]
ATGGAACGAATGATCGAAGACCTGATGGAACAGCTGGTGAATGGAGGCGGAAGCGACCTCCACATCGCGAGCGGACAGCC
GCCTTATGGACGATTCAGTGGTGAACTGCGGCCGATGCAAGAGGAACCGCTCAGTGAAGAAGGCTGCAACCGCCTGATCT
TTTCAATGCTCAACAACAGCCAACGCAAAACACTCGAACAAACGTGGGAGCTGGATTGTGCCTATGGCTTGAAAGGGGTC
GCCCGTTTTCGCGTGAATGTATACCGCCAGAAGGGCAGCTATGCCGCCTGCTTGCGTGCTCTTGGGAGCAAAATCCCCAG
CGTTGAGCTGCTGAATCTGCCTCCGGTCGTCGTGGAAACCAGCAAACGCCCCCGTGGTCTTGTTCTGGTCACGGGCCCGA
CGGGTTCAGGCAAAACCACCACGCTCGCAGCACTTCTCGATCACATCAACCACACACGGGCTGAGCACATCCTCACCATC
GAAGACCCGATCGAATTCGTTTACACCAGTGATAAGAGCTTGGTGCATCAACGCCAACTCAACGAGGACACGCGCAGTTT
TGCCAATGCGCTGCGCGCTGCTTTGCGAGAGGACCCAGACGTGATTTTGGTGGGTGAAATGCGTGATCTCGAAACGATTC
AACTGGCAATCAGCGCTGCGGAAACTGGGCATTTGGTGTTTGAAACTTTGCACACCAGTTCTGCAGCTCAGACCGTTGAT
CGGATGGTGGATGTGTTTCCACCGGGCCAGCAAACTCAAATCCGCGTGCAACTCTCAGGAAGCCTGACGGCGGTGTTCTC
TCAAACGCTCTGCAAACGCCAAAACCCTGCCCCCGGGCAGTTCGGCCGTGTGATGGCTCAAGAAATCATGATCAACACCC
CAGCCATCGCCAATTTGATCCGAGAAGGCAAAACAGCTCAGCTGTATTCCCAAATTCAAACCGGCGGCGAGCGCGGCATG
CAAACCCTCGAAAGAGCTCTTGCAGATCTGATTGATCAAGGCGAAATCAGTCTTGATGAAGGACTCAGCAAAGCCAGCAA
ACCCGCTGAACTGGATCGCCTGATGAACCACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-246)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8F0E7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

53.779

96.359

0.518
  pilT Pseudomonas aeruginosa PAK

53.779

96.359

0.518
  pilT Acinetobacter nosocomialis M2

51.453

96.359

0.496
  pilT Acinetobacter baumannii D1279779

51.453

96.359

0.496
  pilT Acinetobacter baumannii strain A118

51.453

96.359

0.496
  pilT Legionella pneumophila strain Lp02

52.853

93.277

0.493
  pilT Legionella pneumophila strain ERS1305867

52.853

93.277

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.716

98.599

0.49
  pilT Acinetobacter baylyi ADP1

50.872

96.359

0.49
  pilT Neisseria meningitidis 8013

52.252

93.277

0.487
  pilT Neisseria gonorrhoeae MS11

51.952

93.277

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.882

95.238

0.485
  pilT Vibrio cholerae strain A1552

50.882

95.238

0.485
  pilU Pseudomonas stutzeri DSM 10701

39.316

98.319

0.387
  pilU Vibrio cholerae strain A1552

38.617

97.199

0.375
  pilU Acinetobacter baylyi ADP1

37.644

97.479

0.367

Multiple sequence alignment