Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SynMVIR181_RS00100 Genome accession   NZ_CP047942
Coordinates   22905..24677 (-) Length   590 a.a.
NCBI ID   WP_255444333.1    Uniprot ID   A0A7G8F0E8
Organism   Synechococcus sp. MVIR-18-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 17905..29677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynMVIR181_RS00080 (SynMVIR181_00017) msrB 18799..19311 (+) 513 WP_186589552.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynMVIR181_RS00085 (SynMVIR181_00018) - 19238..20554 (+) 1317 WP_186589553.1 NAD(P)/FAD-dependent oxidoreductase -
  SynMVIR181_RS00090 (SynMVIR181_00019) - 20529..21803 (-) 1275 WP_186589554.1 type II secretion system F family protein -
  SynMVIR181_RS00095 (SynMVIR181_00020) pilT 21820..22893 (-) 1074 WP_186590401.1 type IV pilus twitching motility protein PilT Machinery gene
  SynMVIR181_RS00100 (SynMVIR181_00021) pilB/pilB1 22905..24677 (-) 1773 WP_255444333.1 GspE/PulE family protein Machinery gene
  SynMVIR181_RS00105 (SynMVIR181_00022) grpE 24676..25482 (+) 807 WP_186589556.1 nucleotide exchange factor GrpE -
  SynMVIR181_RS00110 (SynMVIR181_00023) dnaJ 25525..26655 (+) 1131 WP_186589557.1 molecular chaperone DnaJ -
  SynMVIR181_RS00115 (SynMVIR181_00024) - 26659..26901 (+) 243 WP_186524236.1 sulfurtransferase TusA family protein -
  SynMVIR181_RS00120 (SynMVIR181_00025) rsgA 26888..27802 (+) 915 WP_370593857.1 ribosome small subunit-dependent GTPase A -
  SynMVIR181_RS00125 (SynMVIR181_00026) - 27777..28118 (-) 342 WP_186589559.1 YbaB/EbfC family nucleoid-associated protein -
  SynMVIR181_RS00130 (SynMVIR181_00027) murB 28143..29081 (-) 939 WP_186589560.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 590 a.a.        Molecular weight: 64308.28 Da        Isoelectric Point: 5.3026

>NTDB_id=418604 SynMVIR181_RS00100 WP_255444333.1 22905..24677(-) (pilB/pilB1) [Synechococcus sp. MVIR-18-1]
MSADSTRMPLPPALPEKLISEAELAAGEAILQSGQVLDLETWKQLQALPIHLTDDGLIVAIASSSDKDSREQLKQVLRSH
GYASKLVLANAAHLKRILDPQEFNSDSTISQTSIIETAKSLLDGFDTEGIFNADPDEAEIQSNSASSLDINPSIAGGSPI
VTLVDRILVKALDMNASDIHVEPQQAGLQIRLRKDGVLTNLTQPLPTKLIPAITSRFKIMADLDIAERRQAQDGRIRRQY
KGRTVDFRVNSLPSRYGEKICLRLLDSQSTQLGLDKLISNPNTLELVRTLGSKPFGMILVTGPTGSGKSTTLYSLLAERN
EPGINISTVEDPIEYTLPGITQCQVNREKGFDFSTALRAFMRQDPDVLLVGETRDQETAKTAIEAALTGHLVLTTLHCND
APSAIARLDEMGVEPFMVSASLLGIVSQRLLRRVCSDCRIPYHPDSQELGRFGLMTSHEGDVTFFKAKQHEGPTTPCPNC
QGTGYKGRIGVYEVLRMNEALAASVAKGATTDLVRQLALEAGMKTLLGYSLDLVREGHTTLEEVDRMVLTDAGLESEQRA
RALNTVTCRGCGGGLHEGWLECPYCLTPRQ

Nucleotide


Download         Length: 1773 bp        

>NTDB_id=418604 SynMVIR181_RS00100 WP_255444333.1 22905..24677(-) (pilB/pilB1) [Synechococcus sp. MVIR-18-1]
ATGAGCGCCGACTCCACCCGAATGCCGCTACCGCCTGCACTGCCGGAAAAGCTGATTTCAGAGGCAGAGCTCGCCGCTGG
AGAAGCAATCCTTCAATCCGGGCAGGTTTTGGACCTTGAGACCTGGAAGCAACTTCAAGCGCTCCCCATCCATCTCACCG
ACGACGGCCTCATCGTGGCCATCGCAAGCAGCAGCGACAAAGACAGTCGAGAACAACTCAAGCAGGTTCTAAGAAGCCAT
GGCTACGCCTCCAAGCTTGTGCTGGCCAATGCAGCGCATCTAAAACGAATTCTTGATCCACAAGAATTCAACTCAGATTC
AACGATTAGCCAAACATCAATTATCGAAACAGCAAAATCGCTTCTCGATGGATTTGATACTGAAGGAATCTTTAATGCTG
ATCCAGACGAAGCTGAAATTCAGAGCAATTCAGCCTCCTCGCTAGACATCAATCCATCCATTGCTGGTGGATCACCGATT
GTCACATTGGTAGATCGAATCTTAGTTAAAGCATTAGACATGAATGCCAGTGATATTCATGTAGAGCCACAACAAGCAGG
ATTACAAATTAGACTTAGAAAGGATGGTGTGCTCACAAATTTAACGCAACCACTCCCCACTAAATTAATACCAGCCATTA
CCTCTCGCTTCAAGATTATGGCTGATCTTGACATCGCTGAACGCAGGCAAGCACAGGATGGGCGTATCCGTAGACAATAC
AAAGGACGAACTGTTGACTTTCGCGTCAACAGTCTTCCCAGCCGCTATGGCGAAAAGATTTGCCTGCGATTACTCGACAG
TCAATCCACTCAATTAGGACTGGATAAGCTCATCTCTAATCCCAACACACTCGAGCTTGTACGAACCCTTGGCTCAAAAC
CATTCGGCATGATTCTTGTGACCGGTCCAACAGGATCTGGTAAATCGACAACACTTTATTCACTGTTAGCGGAGCGCAAC
GAACCCGGAATCAATATCTCTACCGTAGAAGATCCAATTGAATACACGTTGCCTGGTATTACTCAATGTCAGGTGAATCG
AGAGAAAGGATTTGACTTCAGCACGGCCCTTCGCGCCTTCATGCGCCAGGACCCAGATGTTCTGTTAGTGGGTGAGACCC
GTGATCAGGAAACAGCGAAAACTGCTATCGAAGCAGCACTGACAGGGCACCTGGTCTTGACCACCCTGCACTGCAACGAT
GCACCGAGCGCCATCGCCCGACTCGACGAAATGGGCGTGGAACCGTTCATGGTGAGCGCATCCTTGCTCGGCATCGTTTC
TCAACGCTTGCTCAGGCGCGTGTGCAGCGATTGCCGCATCCCTTACCACCCAGATTCCCAAGAATTAGGTCGCTTTGGGC
TGATGACGAGCCATGAAGGCGATGTGACCTTTTTCAAAGCCAAACAGCATGAAGGACCAACAACGCCATGCCCCAACTGT
CAGGGCACCGGTTACAAGGGCCGTATCGGTGTGTATGAAGTGCTGCGCATGAATGAAGCCCTGGCCGCATCTGTCGCCAA
AGGCGCCACCACTGATCTTGTCCGCCAGTTGGCACTGGAAGCAGGAATGAAAACCTTGCTTGGCTACAGCCTTGATCTCG
TGAGAGAGGGTCACACCACCCTGGAAGAGGTAGACCGGATGGTCTTAACAGATGCGGGCCTGGAATCGGAACAACGTGCT
AGGGCTCTCAACACAGTCACTTGCCGTGGATGTGGTGGCGGACTGCATGAAGGTTGGCTGGAATGCCCGTATTGCCTGAC
ACCTCGTCAGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-99)

Type II/IV secretion system protein (T2SSE)

(167-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8F0E8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

53.94

96.78

0.522

Multiple sequence alignment