Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynNOUM97013_RS00095 Genome accession   NZ_CP047941
Coordinates   22293..23369 (-) Length   358 a.a.
NCBI ID   WP_186480270.1    Uniprot ID   A0A7G8ES30
Organism   Synechococcus sp. NOUM97013     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 17293..28369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynNOUM97013_RS00070 (SynNOUM97013_00014) - 17495..18025 (+) 531 WP_186480266.1 RNA-binding protein -
  SynNOUM97013_RS00075 (SynNOUM97013_00015) dusA 18044..19048 (-) 1005 WP_186480267.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynNOUM97013_RS00080 (SynNOUM97013_00016) msrB 19118..19627 (+) 510 WP_186480268.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynNOUM97013_RS00085 (SynNOUM97013_00017) - 19662..20852 (+) 1191 WP_370586482.1 NAD(P)/FAD-dependent oxidoreductase -
  SynNOUM97013_RS00090 (SynNOUM97013_00018) - 20860..22290 (-) 1431 WP_255442841.1 type II secretion system F family protein -
  SynNOUM97013_RS00095 (SynNOUM97013_00019) pilT 22293..23369 (-) 1077 WP_186480270.1 type IV pilus twitching motility protein PilT Machinery gene
  SynNOUM97013_RS00100 (SynNOUM97013_00020) pilB/pilB1 23380..25116 (-) 1737 WP_186480271.1 GspE/PulE family protein Machinery gene
  SynNOUM97013_RS00105 (SynNOUM97013_00021) grpE 25211..25936 (+) 726 WP_186481314.1 nucleotide exchange factor GrpE -
  SynNOUM97013_RS00110 (SynNOUM97013_00022) dnaJ 25979..27109 (+) 1131 WP_186480272.1 molecular chaperone DnaJ -
  SynNOUM97013_RS00115 (SynNOUM97013_00023) - 27112..27354 (+) 243 WP_186480273.1 sulfurtransferase TusA family protein -
  SynNOUM97013_RS00120 (SynNOUM97013_00024) rsgA 27374..28255 (+) 882 WP_186481315.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39517.23 Da        Isoelectric Point: 6.3946

>NTDB_id=418592 SynNOUM97013_RS00095 WP_186480270.1 22293..23369(-) (pilT) [Synechococcus sp. NOUM97013]
MELMIEDLMQELVEAGGSDLHIASGQPPYGRFSGELRPMRDEPLLEESCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSSIPSIDRLNLPPVVVQTCERPRGLVLVTGPTGSGKTTTLAALLDHINHSRAEHILTI
EDPIEFVYKSDRSLVHQRQLNEDTRSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQTQIRVQLSGSLVAVFSQTLCRRSNPAPGQFGRVMAQEILINTAATANLIREGKTAQLYSQIQTGGELGM
QTLEKALADLLKRKQISKAEAMAKASKPGELERLIQEL

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=418592 SynNOUM97013_RS00095 WP_186480270.1 22293..23369(-) (pilT) [Synechococcus sp. NOUM97013]
ATGGAGCTGATGATCGAAGACCTGATGCAGGAGCTTGTCGAGGCCGGCGGCAGCGACCTGCACATCGCCAGTGGGCAGCC
CCCCTACGGCCGCTTCAGCGGCGAACTGCGTCCGATGCGGGACGAACCTCTGCTGGAGGAGAGCTGCAACCGGCTGATCT
TCTCCATGCTCAACAACAGTCAGCGCAAGACACTCGAACAAACGTGGGAACTTGACTGCGCCTACGGACTGAAGGGCGTG
GCCCGCTTCCGGGTGAATGTGTACCGGCAGAAGGGAAGCTATGCCGCCTGTCTGCGGGCCCTCGGCAGCAGCATTCCCAG
CATCGATCGGCTGAATCTGCCGCCGGTGGTGGTGCAGACCTGCGAACGACCACGGGGCCTTGTGCTGGTCACAGGACCGA
CCGGCTCGGGAAAGACCACCACCCTGGCGGCGCTGCTCGATCACATCAATCACAGCCGCGCCGAACACATCCTCACCATC
GAGGATCCGATCGAGTTCGTGTACAAGAGCGACCGCAGCCTGGTGCATCAGCGGCAGCTGAATGAAGACACGCGCAGCTT
CGCCAACGCCCTCCGTGCGGCGTTGCGCGAGGACCCGGACGTGATCCTGGTGGGTGAAATGCGGGATCTGGAAACGATTC
AACTGGCGATCAGCGCAGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGTTCGGCCGCGCAGACCGTGGAC
CGCATGGTGGATGTGTTTCCCCCCGAGCAGCAGACCCAGATCCGCGTGCAACTCTCCGGAAGCCTGGTGGCTGTGTTCTC
GCAGACCCTCTGTCGCCGCAGCAACCCAGCCCCGGGCCAGTTCGGGCGGGTGATGGCCCAGGAAATCCTGATCAACACCG
CCGCCACAGCCAACCTGATCCGCGAAGGCAAGACCGCACAGCTGTATTCCCAGATCCAGACCGGAGGAGAACTGGGCATG
CAGACCCTTGAGAAAGCGCTGGCGGATCTGTTGAAACGCAAGCAGATCTCCAAGGCGGAAGCCATGGCCAAGGCCTCGAA
ACCCGGGGAGCTGGAACGACTGATCCAAGAGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8ES30

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

52.326

96.089

0.503
  pilT Pseudomonas aeruginosa PAK

52.035

96.089

0.5
  pilT Legionella pneumophila strain Lp02

51.929

94.134

0.489
  pilT Legionella pneumophila strain ERS1305867

51.929

94.134

0.489
  pilT Acinetobacter baumannii strain A118

50.581

96.089

0.486
  pilT Acinetobacter nosocomialis M2

50.581

96.089

0.486
  pilT Acinetobacter baumannii D1279779

50.581

96.089

0.486
  pilT Acinetobacter baylyi ADP1

50.291

96.089

0.483
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.008

98.603

0.483
  pilT Neisseria meningitidis 8013

49.271

95.81

0.472
  pilT Neisseria gonorrhoeae MS11

48.98

95.81

0.469
  pilT Vibrio cholerae strain A1552

48.529

94.972

0.461
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.529

94.972

0.461
  pilU Pseudomonas stutzeri DSM 10701

38.068

98.324

0.374
  pilU Acinetobacter baylyi ADP1

37.644

97.207

0.366
  pilU Vibrio cholerae strain A1552

38.235

94.972

0.363

Multiple sequence alignment