Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynBMKMC1_RS00095 Genome accession   NZ_CP047939
Coordinates   21792..22865 (-) Length   357 a.a.
NCBI ID   WP_186472262.1    Uniprot ID   A0A7G8EA00
Organism   Synechococcus sp. BMK-MC-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 16792..27865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynBMKMC1_RS00070 (SynBMKMC1_00014) - 17077..17661 (+) 585 WP_186472258.1 RNA-binding protein -
  SynBMKMC1_RS00075 (SynBMKMC1_00015) dusA 17680..18684 (-) 1005 WP_011931950.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynBMKMC1_RS00080 (SynBMKMC1_00016) msrB 18745..19263 (+) 519 WP_186472259.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynBMKMC1_RS00085 (SynBMKMC1_00017) - 19298..20497 (+) 1200 WP_370593961.1 NAD(P)/FAD-dependent oxidoreductase -
  SynBMKMC1_RS00090 (SynBMKMC1_00018) - 20519..21775 (-) 1257 WP_186472261.1 type II secretion system F family protein -
  SynBMKMC1_RS00095 (SynBMKMC1_00019) pilT 21792..22865 (-) 1074 WP_186472262.1 type IV pilus twitching motility protein PilT Machinery gene
  SynBMKMC1_RS00100 (SynBMKMC1_00020) pilB/pilB1 22876..24561 (-) 1686 WP_186472263.1 GspE/PulE family protein Machinery gene
  SynBMKMC1_RS00105 (SynBMKMC1_00021) grpE 24657..25379 (+) 723 WP_255486232.1 nucleotide exchange factor GrpE -
  SynBMKMC1_RS00110 (SynBMKMC1_00022) dnaJ 25430..26560 (+) 1131 WP_186472265.1 molecular chaperone DnaJ -
  SynBMKMC1_RS00115 (SynBMKMC1_00023) - 26563..26805 (+) 243 WP_186472266.1 sulfurtransferase TusA family protein -
  SynBMKMC1_RS00120 (SynBMKMC1_00024) rsgA 26825..27706 (+) 882 WP_050815906.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39145.75 Da        Isoelectric Point: 6.3937

>NTDB_id=418570 SynBMKMC1_RS00095 WP_186472262.1 21792..22865(-) (pilT) [Synechococcus sp. BMK-MC-1]
MEVMIEDLMQELVEAGGSDLHIASGQPPYGRFSGQLRPMRDEPLMEESCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSKIPSIELLNLPPVVVETSKRPRGLVLVTGPTGSGKTTTLAALLDHINHSRAEHILTI
EDPIEFVYKSDQSLVHQRQLNEDTRSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPAQQTQIRVQLSGSLVAVFSQTLCRRSNPAPGQFGRVMAQEIMINTPAIANLIREGKTAQLYSQIQTGGELGM
QTLEKALADLVSRGDISLQEARAKASKPSELDRLAAG

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=418570 SynBMKMC1_RS00095 WP_186472262.1 21792..22865(-) (pilT) [Synechococcus sp. BMK-MC-1]
ATGGAAGTGATGATCGAAGACCTGATGCAGGAGCTGGTGGAAGCGGGCGGTAGCGACCTGCACATCGCCAGCGGTCAACC
CCCTTACGGCCGCTTCAGCGGCCAGTTGCGGCCCATGCGCGACGAGCCCCTGATGGAAGAAAGCTGCAACCGGCTGATCT
TTTCCATGCTCAACAACAGCCAGCGCAAAACGCTCGAGCAAACCTGGGAGCTCGACTGTGCCTATGGACTCAAAGGCGTG
GCCCGCTTCCGGGTGAACGTGTATCGCCAGAAGGGCAGCTATGCGGCCTGTCTGCGAGCTCTTGGCAGCAAAATCCCCAG
CATCGAGCTGCTGAATCTGCCCCCCGTGGTGGTGGAAACCAGCAAACGACCCCGGGGATTGGTGCTCGTGACCGGCCCGA
CTGGGTCAGGCAAAACCACCACCCTCGCGGCCCTGCTGGATCACATCAATCACAGCCGCGCCGAGCACATCCTCACGATC
GAGGATCCGATCGAATTCGTTTACAAAAGCGATCAGAGCCTGGTGCACCAACGCCAGCTCAATGAAGACACCCGCAGTTT
CGCCAATGCACTGCGAGCAGCCCTCAGGGAAGACCCCGACGTGATCCTGGTGGGAGAAATGCGCGATCTGGAAACCATCC
AGCTGGCGATCAGCGCTGCTGAAACCGGGCACCTGGTGTTCGGCACCCTGCACACCAGCTCAGCGGCCCAAACCGTGGAC
CGCATGGTGGATGTGTTCCCCCCCGCGCAACAAACCCAGATCCGCGTGCAACTCTCAGGGAGCCTGGTGGCGGTGTTCTC
CCAGACCCTGTGCCGCCGCAGCAACCCGGCGCCTGGGCAATTCGGTCGGGTGATGGCGCAGGAAATCATGATCAACACAC
CGGCCATCGCCAATCTCATCCGCGAAGGAAAAACCGCCCAGCTGTATTCGCAGATTCAGACCGGTGGCGAACTGGGCATG
CAGACCCTGGAGAAGGCTCTGGCGGATCTCGTCAGCCGAGGAGACATCAGCCTGCAGGAAGCCAGAGCCAAAGCCAGCAA
ACCATCCGAACTGGATCGGCTCGCGGCCGGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8EA00

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.89

97.199

0.524
  pilT Pseudomonas stutzeri DSM 10701

53.026

97.199

0.515
  pilT Acinetobacter nosocomialis M2

52.161

97.199

0.507
  pilT Legionella pneumophila strain Lp02

52.174

96.639

0.504
  pilT Legionella pneumophila strain ERS1305867

52.174

96.639

0.504
  pilT Acinetobacter baumannii strain A118

51.873

97.199

0.504
  pilT Acinetobacter baumannii D1279779

51.873

97.199

0.504
  pilT Acinetobacter baylyi ADP1

51.437

97.479

0.501
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.288

98.319

0.485
  pilT Neisseria meningitidis 8013

50.44

95.518

0.482
  pilT Neisseria gonorrhoeae MS11

50.147

95.518

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

95.238

0.476
  pilT Vibrio cholerae strain A1552

50

95.238

0.476
  pilU Pseudomonas stutzeri DSM 10701

38.905

97.199

0.378
  pilU Vibrio cholerae strain A1552

39.104

93.838

0.367
  pilU Acinetobacter baylyi ADP1

37.356

97.479

0.364

Multiple sequence alignment