Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SynBMKMC1_RS00100 Genome accession   NZ_CP047939
Coordinates   22876..24561 (-) Length   561 a.a.
NCBI ID   WP_186472263.1    Uniprot ID   -
Organism   Synechococcus sp. BMK-MC-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 17876..29561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynBMKMC1_RS00080 (SynBMKMC1_00016) msrB 18745..19263 (+) 519 WP_186472259.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynBMKMC1_RS00085 (SynBMKMC1_00017) - 19298..20497 (+) 1200 WP_370593961.1 NAD(P)/FAD-dependent oxidoreductase -
  SynBMKMC1_RS00090 (SynBMKMC1_00018) - 20519..21775 (-) 1257 WP_186472261.1 type II secretion system F family protein -
  SynBMKMC1_RS00095 (SynBMKMC1_00019) pilT 21792..22865 (-) 1074 WP_186472262.1 type IV pilus twitching motility protein PilT Machinery gene
  SynBMKMC1_RS00100 (SynBMKMC1_00020) pilB/pilB1 22876..24561 (-) 1686 WP_186472263.1 GspE/PulE family protein Machinery gene
  SynBMKMC1_RS00105 (SynBMKMC1_00021) grpE 24657..25379 (+) 723 WP_255486232.1 nucleotide exchange factor GrpE -
  SynBMKMC1_RS00110 (SynBMKMC1_00022) dnaJ 25430..26560 (+) 1131 WP_186472265.1 molecular chaperone DnaJ -
  SynBMKMC1_RS00115 (SynBMKMC1_00023) - 26563..26805 (+) 243 WP_186472266.1 sulfurtransferase TusA family protein -
  SynBMKMC1_RS00120 (SynBMKMC1_00024) rsgA 26825..27706 (+) 882 WP_050815906.1 ribosome small subunit-dependent GTPase A -
  SynBMKMC1_RS00125 (SynBMKMC1_00025) - 27681..28022 (-) 342 WP_011931961.1 YbaB/EbfC family nucleoid-associated protein -
  SynBMKMC1_RS00130 (SynBMKMC1_00026) murB 28047..28979 (-) 933 WP_186472267.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 61462.80 Da        Isoelectric Point: 4.8123

>NTDB_id=418571 SynBMKMC1_RS00100 WP_186472263.1 22876..24561(-) (pilB/pilB1) [Synechococcus sp. BMK-MC-1]
MSDDRRRLELELLLKRTIPGTLSSADKPDLLNDLTALGLDPKRHGELTATLSSLLEPAPAEALQEQKQEQQQEHAQAPQQ
APESIADTASSYLAEFSVDGVLEDDPDEAELQSSSIVDLEDGLNDADRSPVINLVDRILMQALQLGASDIHVEPQQAGLQ
LRFRQDGVLQTSIEPLPSRLVPAVTSRFKIMADLDIAERRVAQDGRIRRRFQNRTVDFRVNCLPSRFGEKIVLRLLDSSA
TQLGLDKLITSETTLDTVRSLGSKPFGMILVTGPTGSGKSTTLYSLLAERNDPGINISTVEDPIEYTLPGITQCQVNREK
GFDFSQALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDAPSAIARLDEMGVEPFMVSASLIGIVSQR
LLRRVCPDCRLAYHPKAEELGRFGLMTSNEEGVTFFKANHREGHPDACPSCQGSGYKGRVGVYEVLRMNEVMAAAVAKGA
TTDMVRQLALESGMKTLLGYSLDLVREGRTTLEEVGRMVLTDAGLESERRARALRTLTCSGCGGGLQEGWLECPYCLTPR
H

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=418571 SynBMKMC1_RS00100 WP_186472263.1 22876..24561(-) (pilB/pilB1) [Synechococcus sp. BMK-MC-1]
GTGAGTGACGATCGCCGCCGACTGGAGCTGGAACTGCTGCTGAAACGCACCATCCCAGGCACCCTCAGCAGCGCCGACAA
ACCGGATCTTCTGAACGATTTGACAGCGCTGGGACTGGATCCGAAGCGCCACGGCGAGCTCACAGCCACCCTGAGCAGCC
TTCTCGAACCAGCTCCGGCAGAAGCACTCCAAGAGCAGAAGCAAGAACAACAGCAGGAACACGCGCAAGCTCCTCAGCAA
GCACCGGAGAGCATTGCCGACACCGCCAGCTCCTATCTCGCGGAATTCAGCGTGGACGGAGTGCTGGAGGACGACCCCGA
TGAGGCCGAACTGCAGTCGTCTTCGATCGTGGATCTGGAAGACGGACTGAACGATGCCGATCGTTCACCGGTGATCAATC
TCGTGGACCGGATCCTGATGCAGGCGCTGCAGCTGGGAGCCAGCGACATTCATGTGGAGCCCCAGCAGGCTGGCTTGCAG
TTGCGGTTTCGCCAGGACGGCGTGCTGCAAACCAGCATCGAGCCCCTGCCAAGCCGTTTGGTGCCAGCGGTGACCTCACG
CTTCAAAATCATGGCGGATCTGGATATCGCCGAGCGCAGGGTGGCCCAGGACGGGCGGATTCGGCGCAGATTCCAGAACC
GCACCGTGGACTTTCGGGTGAATTGCCTACCCAGTCGCTTCGGGGAGAAGATCGTGCTGCGACTGCTGGACAGCTCCGCC
ACCCAACTGGGCCTGGACAAGCTGATTACCAGTGAGACAACGCTCGACACCGTGCGGTCCCTGGGCTCCAAACCCTTCGG
CATGATCCTGGTGACCGGACCCACCGGATCGGGGAAATCGACCACGCTCTATTCCCTGCTGGCCGAACGCAACGACCCTG
GAATCAACATCTCCACGGTGGAAGATCCGATCGAATACACCCTCCCCGGCATCACCCAATGCCAGGTGAACCGAGAGAAA
GGGTTCGATTTCAGCCAGGCGCTGCGGGCCTTCATGCGCCAAGACCCGGATGTGCTGCTCGTGGGCGAGACCCGCGACCT
GGAAACGGCCAAAACCGCCATCGAGGCAGCACTCACCGGTCACCTCGTGCTCACCACCTTGCATTGCAACGACGCACCCA
GCGCCATCGCCCGACTGGATGAAATGGGCGTTGAGCCCTTCATGGTGAGCGCGTCGCTGATCGGCATCGTTTCCCAACGC
CTGCTGCGGCGTGTCTGCCCCGACTGCCGCCTTGCCTATCACCCGAAAGCTGAGGAGCTCGGGCGGTTCGGCTTGATGAC
CAGCAATGAAGAGGGCGTGACGTTCTTCAAGGCCAATCACCGCGAGGGACATCCGGACGCTTGTCCCAGCTGTCAGGGCA
GCGGCTACAAGGGCCGGGTGGGGGTGTACGAGGTGCTGCGCATGAATGAAGTGATGGCCGCTGCAGTAGCCAAGGGAGCC
ACCACCGACATGGTGCGCCAGCTGGCCCTGGAATCAGGAATGAAAACGCTGCTGGGCTACAGCCTCGATCTCGTGCGCGA
AGGACGCACCACCCTGGAGGAAGTGGGCCGCATGGTGCTCACCGATGCCGGCCTGGAGTCCGAACGGCGGGCCCGAGCCC
TGAGAACGCTCACCTGCAGCGGATGTGGTGGCGGACTTCAGGAAGGTTGGCTGGAATGTCCGTACTGCCTTACCCCACGC
CATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(138-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.004

80.749

0.533

Multiple sequence alignment