Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SynA1528_RS00095 Genome accession   NZ_CP047931
Coordinates   21564..22640 (-) Length   358 a.a.
NCBI ID   WP_186587138.1    Uniprot ID   -
Organism   Synechococcus sp. A15-28     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 16564..27640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynA1528_RS00070 (SynA1528_00014) - 16857..17519 (+) 663 WP_186587134.1 RNA-binding protein -
  SynA1528_RS00075 (SynA1528_00015) dusA 17525..18517 (-) 993 WP_186587135.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SynA1528_RS00080 (SynA1528_00016) msrB 18559..19071 (+) 513 WP_186587136.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynA1528_RS00085 (SynA1528_00017) - 18992..20314 (+) 1323 WP_186587137.1 NAD(P)/FAD-dependent oxidoreductase -
  SynA1528_RS00090 (SynA1528_00018) - 20289..21488 (-) 1200 WP_286187965.1 type II secretion system F family protein -
  SynA1528_RS00095 (SynA1528_00019) pilT 21564..22640 (-) 1077 WP_186587138.1 type IV pilus twitching motility protein PilT Machinery gene
  SynA1528_RS00100 (SynA1528_00020) pilB/pilB1 22651..24264 (-) 1614 WP_286187966.1 GspE/PulE family protein Machinery gene
  SynA1528_RS00105 (SynA1528_00021) - 24367..24522 (-) 156 WP_186587140.1 general secretion pathway protein GspE -
  SynA1528_RS00110 (SynA1528_00022) grpE 24622..25278 (+) 657 WP_186587141.1 nucleotide exchange factor GrpE -
  SynA1528_RS00115 (SynA1528_00023) dnaJ 25278..26411 (+) 1134 WP_186587142.1 molecular chaperone DnaJ -
  SynA1528_RS00120 (SynA1528_00024) - 26411..26650 (+) 240 WP_186587143.1 sulfurtransferase TusA family protein -
  SynA1528_RS00125 (SynA1528_00025) rsgA 26647..27546 (+) 900 WP_186587144.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39505.95 Da        Isoelectric Point: 5.6446

>NTDB_id=418507 SynA1528_RS00095 WP_186587138.1 21564..22640(-) (pilT) [Synechococcus sp. A15-28]
MDLMIEDLMEQLVEAGGSDLHIASGQPPYERFSGELRPMRDDPLEEEDCNKLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRKKGSYAACLRALGSKIPSVELLNLPPVVLETSKRPRGLVLVTGPTGSGKTTTLAALLDHINHTRSEHILTI
EDPIEFVYQSDKSLVHQRQLNEDTRSFANALRAALREDPDVILVGEIRDLETIQLAVSAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQTQVRVQLSGSLVAVFSQTLCKRHDPKPGQFGRVMAQEILINTPATANLIREGKTAQLYSQIQTGGEQGM
QTLEKALADLVNRGDVSQTEAMAKASKPGELQRLIGHE

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=418507 SynA1528_RS00095 WP_186587138.1 21564..22640(-) (pilT) [Synechococcus sp. A15-28]
ATGGATCTGATGATCGAGGATCTGATGGAGCAACTGGTGGAGGCAGGCGGCAGCGATCTGCACATCGCCAGCGGTCAGCC
TCCCTACGAACGTTTCAGCGGTGAGCTGCGGCCGATGCGGGACGACCCTCTGGAGGAGGAGGACTGCAACAAGCTGATCT
TCTCGATGCTGAACAACAGTCAACGCAAAACCCTGGAGCAGACCTGGGAACTGGACTGCGCCTATGGCCTGAAAGGGGTG
GCACGGTTCCGGGTCAACGTCTACCGGAAGAAGGGAAGCTATGCCGCCTGTCTCCGGGCCCTCGGCAGCAAGATCCCAAG
CGTTGAGCTTCTGAACCTGCCGCCCGTGGTGCTGGAGACCAGCAAACGCCCGCGGGGACTGGTGCTGGTGACAGGTCCCA
CAGGGTCGGGCAAAACCACGACGCTGGCAGCCCTGTTGGACCACATCAATCACACCCGCAGCGAACACATCCTCACCATC
GAGGACCCGATTGAGTTTGTTTATCAGAGCGACAAGAGCCTGGTACATCAGCGCCAGCTCAATGAGGACACCCGCAGTTT
CGCCAATGCCCTCCGCGCTGCTCTGCGGGAGGACCCCGACGTGATCCTGGTGGGGGAAATTCGGGATCTGGAAACCATCC
AACTGGCAGTCAGCGCTGCGGAGACCGGACACCTTGTGTTCGGAACGCTGCACACCAGTTCGGCGGCGCAGACCGTGGAC
CGGATGGTCGATGTATTCCCACCGGAACAACAGACCCAGGTCCGCGTTCAACTCTCCGGCAGCTTGGTGGCCGTGTTTTC
ACAGACCCTCTGCAAGCGCCACGATCCAAAACCAGGGCAATTCGGACGGGTGATGGCTCAGGAGATCCTGATCAACACCC
CGGCCACCGCAAATCTCATCCGCGAAGGCAAGACCGCTCAGCTTTATTCCCAGATTCAGACCGGTGGCGAGCAAGGAATG
CAGACCCTGGAAAAAGCGCTGGCTGACCTGGTGAACCGCGGCGATGTGAGCCAGACCGAAGCCATGGCAAAAGCGAGCAA
ACCAGGCGAACTGCAACGCCTGATTGGCCATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.651

96.089

0.525
  pilT Pseudomonas stutzeri DSM 10701

53.779

96.089

0.517
  pilT Legionella pneumophila strain ERS1305867

54.006

94.134

0.508
  pilT Legionella pneumophila strain Lp02

54.006

94.134

0.508
  pilT Acinetobacter nosocomialis M2

52.326

96.089

0.503
  pilT Acinetobacter baumannii D1279779

52.326

96.089

0.503
  pilT Acinetobacter baumannii strain A118

52.326

96.089

0.503
  pilT Neisseria meningitidis 8013

51.603

95.81

0.494
  pilT Neisseria gonorrhoeae MS11

51.312

95.81

0.492
  pilT Acinetobacter baylyi ADP1

50.581

96.089

0.486
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.429

97.765

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.588

94.972

0.48
  pilT Vibrio cholerae strain A1552

50.588

94.972

0.48
  pilU Pseudomonas stutzeri DSM 10701

39.031

98.045

0.383
  pilU Acinetobacter baylyi ADP1

37.822

97.486

0.369
  pilU Vibrio cholerae strain A1552

38.348

94.693

0.363

Multiple sequence alignment