Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SynA1528_RS00100 Genome accession   NZ_CP047931
Coordinates   22651..24264 (-) Length   537 a.a.
NCBI ID   WP_286187966.1    Uniprot ID   -
Organism   Synechococcus sp. A15-28     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 17651..29264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynA1528_RS00080 (SynA1528_00016) msrB 18559..19071 (+) 513 WP_186587136.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynA1528_RS00085 (SynA1528_00017) - 18992..20314 (+) 1323 WP_186587137.1 NAD(P)/FAD-dependent oxidoreductase -
  SynA1528_RS00090 (SynA1528_00018) - 20289..21488 (-) 1200 WP_286187965.1 type II secretion system F family protein -
  SynA1528_RS00095 (SynA1528_00019) pilT 21564..22640 (-) 1077 WP_186587138.1 type IV pilus twitching motility protein PilT Machinery gene
  SynA1528_RS00100 (SynA1528_00020) pilB/pilB1 22651..24264 (-) 1614 WP_286187966.1 GspE/PulE family protein Machinery gene
  SynA1528_RS00105 (SynA1528_00021) - 24367..24522 (-) 156 WP_186587140.1 general secretion pathway protein GspE -
  SynA1528_RS00110 (SynA1528_00022) grpE 24622..25278 (+) 657 WP_186587141.1 nucleotide exchange factor GrpE -
  SynA1528_RS00115 (SynA1528_00023) dnaJ 25278..26411 (+) 1134 WP_186587142.1 molecular chaperone DnaJ -
  SynA1528_RS00120 (SynA1528_00024) - 26411..26650 (+) 240 WP_186587143.1 sulfurtransferase TusA family protein -
  SynA1528_RS00125 (SynA1528_00025) rsgA 26647..27546 (+) 900 WP_186587144.1 ribosome small subunit-dependent GTPase A -
  SynA1528_RS00130 (SynA1528_00026) - 27521..27862 (-) 342 WP_186587145.1 YbaB/EbfC family nucleoid-associated protein -
  SynA1528_RS00135 (SynA1528_00027) murB 27887..28786 (-) 900 WP_186587146.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 537 a.a.        Molecular weight: 58849.50 Da        Isoelectric Point: 4.8855

>NTDB_id=418508 SynA1528_RS00100 WP_286187966.1 22651..24264(-) (pilB/pilB1) [Synechococcus sp. A15-28]
MPPNDRTIRLHPTLHDDLRRALATEPSERSTPSAPGEAPRETQAITTSQPSQNRTESQLSLEDEASSFLEDFNAEGVLET
AEDEEAALSANAVDLESSLEDPDASPVVALVDRILLQAMSVGASDIHVEPQQKGLRLRYRQDGVLQQYVEPLPNRLIPAV
TSRFKILADLDIAERRQAQDGRISRRYRDRVVDFRVNTLPSRFGEKVCLRLLDSSATQLGLDKLISNPATLALVRDLGAK
PFGMILVTGPTGSGKSTTLYSLLAERNDPGINISTVEDPIEYTLPGITQCQVNRDKGFDFATALRAFMRQDPDVLLVGET
RDLETAKTAIEAALTGHLVLSTLHANDAPSTIARLDEMGVETFMVSAALIGIVSQRLMRRVCPTCREPYRPDERELGRFG
LMASTESDVTFFRAHRHDGSGKTCPNCKGSGYKGRVGVYEVLRMNEELATAVSSGASTDVIRQLALESGMVTLLGYSLDL
VRQGHTTLEEVGRMILTDSGLESERRARALSTMTCKGCGAGLQESWLECPYCLTTRD

Nucleotide


Download         Length: 1614 bp        

>NTDB_id=418508 SynA1528_RS00100 WP_286187966.1 22651..24264(-) (pilB/pilB1) [Synechococcus sp. A15-28]
CTGCCGCCGAATGACCGAACCATCCGTCTGCACCCGACGCTGCATGACGATCTGCGGCGTGCTCTGGCGACTGAACCATC
GGAACGATCTACCCCGTCAGCACCTGGTGAAGCTCCCCGTGAAACTCAAGCGATAACAACCAGTCAGCCAAGTCAAAACA
GGACTGAGAGCCAGTTGTCCCTCGAAGACGAAGCATCCTCGTTCCTCGAAGACTTCAACGCCGAAGGGGTGCTGGAGACC
GCAGAAGACGAAGAGGCAGCCCTGTCCGCCAATGCCGTTGATCTGGAATCCAGCCTTGAGGACCCTGATGCATCACCGGT
GGTGGCCCTGGTGGATCGCATCCTGCTGCAGGCCATGTCCGTGGGCGCCTCCGACATCCATGTGGAGCCGCAACAGAAGG
GTCTCCGTCTGCGCTACCGCCAGGACGGTGTGCTCCAGCAGTACGTGGAGCCGCTTCCTAACCGGTTGATCCCAGCGGTG
ACCTCCCGCTTCAAGATCCTGGCGGACCTCGATATCGCTGAGCGACGCCAGGCTCAGGACGGCAGGATCAGTCGCCGCTA
CCGGGACCGGGTGGTGGATTTCAGGGTCAACACCCTGCCCAGCCGCTTCGGCGAAAAGGTTTGTCTGCGACTGCTGGACA
GCAGCGCCACGCAACTGGGCCTGGACAAGCTGATCTCCAATCCCGCCACCCTCGCTCTTGTGCGGGATCTGGGGGCCAAA
CCCTTCGGCATGATTCTGGTCACAGGGCCCACCGGCTCAGGCAAATCCACCACCCTCTATTCCCTGCTGGCGGAACGCAA
CGACCCCGGCATCAACATCTCCACGGTGGAGGATCCGATTGAGTACACGCTGCCGGGCATCACGCAGTGCCAGGTGAATC
GCGACAAGGGATTCGATTTCGCCACTGCCCTGAGGGCATTCATGCGCCAGGACCCGGATGTGCTGCTGGTCGGGGAGACC
CGCGATCTGGAGACAGCGAAAACCGCCATCGAAGCCGCCCTCACCGGCCACCTGGTGCTGAGCACCCTTCACGCCAATGA
CGCCCCCAGCACCATCGCCCGACTGGATGAGATGGGGGTGGAGACCTTCATGGTGTCGGCAGCCCTGATCGGCATCGTGT
CCCAACGGCTGATGCGACGCGTTTGCCCGACGTGCCGGGAACCGTACCGGCCGGATGAGCGAGAACTTGGTCGCTTCGGC
CTGATGGCCAGCACGGAAAGCGATGTGACCTTTTTCAGGGCCCATCGCCATGACGGCAGCGGCAAGACCTGCCCCAATTG
CAAAGGCAGTGGTTACAAGGGCCGCGTGGGTGTCTACGAAGTGCTGCGCATGAATGAGGAGCTGGCCACAGCCGTTTCCT
CAGGCGCCTCCACCGATGTGATCCGTCAACTCGCGCTCGAGTCAGGGATGGTGACTCTTCTCGGCTACAGCCTGGATCTC
GTCCGACAGGGTCACACCACGCTGGAAGAAGTTGGGCGAATGATCCTCACCGACTCAGGCCTGGAATCCGAGCGCAGAGC
ACGCGCTCTCAGCACAATGACCTGCAAGGGATGCGGCGCTGGTCTCCAGGAGAGCTGGCTGGAGTGTCCTTATTGCCTAA
CCACCCGAGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

62.232

86.778

0.54
  pilB Vibrio campbellii strain DS40M4

47.281

78.771

0.372
  pilB Vibrio parahaemolyticus RIMD 2210633

46.809

78.771

0.369
  pilB Vibrio cholerae strain A1552

47.143

78.212

0.369

Multiple sequence alignment