Detailed information NTG0418009

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: H9199_RS05585
• Genome accession: NZ_CP060933
• Coordinates: 1121754..1122734 (+)
• Length: 326 a.a.
• NCBI ID: WP_072649429.1
• Uniprot ID: -
• Organism: Escherichia coli strain EC12
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1116754..1127734

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H9199_RS05555 (H9199_05460)	yggM	1117269..1118276 (+)	1008	WP_000745217.1	DUF1202 family protein	-
H9199_RS05560 (H9199_05465)	hemW	1118431..1119567 (-)	1137	WP_000239944.1	radical SAM family heme chaperone HemW	-
H9199_RS05565 (H9199_05470)	rdgB	1119560..1120153 (-)	594	WP_042630986.1	XTP/dITP diphosphatase	-
H9199_RS05570 (H9199_05475)	yggU	1120161..1120451 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
H9199_RS05575 (H9199_05480)	yggT	1120448..1121014 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
H9199_RS05580 (H9199_05485)	yggS	1121032..1121736 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
H9199_RS05585 (H9199_05490)	pilT	1121754..1122734 (+)	981	WP_072649429.1	type IV pilus twitching motility protein PilT	Machinery gene
H9199_RS05590 (H9199_05495)	ruvX	1122910..1123326 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
H9199_RS05595 (H9199_05500)	yqgE	1123326..1123889 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
H9199_RS05600 (H9199_05505)	gshB	1123998..1124948 (-)	951	WP_000593273.1	glutathione synthase	-
H9199_RS05605 (H9199_05510)	rsmE	1124961..1125692 (-)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
H9199_RS05610 (H9199_05515)	endA	1125772..1126479 (-)	708	WP_000286500.1	deoxyribonuclease I	-
H9199_RS05615 (H9199_05520)	yggI	1126574..1127071 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35918.05 Da
• Isoelectric Point: 5.5860

>NTDB_id=418009 H9199_RS05585 WP_072649429.1 1121754..1122734(+) (pilT) [Escherichia coli strain EC12]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAQFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=418009 H9199_RS05585 WP_072649429.1 1121754..1122734(+) (pilT) [Escherichia coli strain EC12]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCAATTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	39.514	100	0.399
pilU	Pseudomonas stutzeri DSM 10701	37.879	100	0.383
pilU	Acinetobacter baylyi ADP1	37.037	99.387	0.368
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362