Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NIT79A3_RS00590 Genome accession   NC_015731
Coordinates   141536..142579 (-) Length   347 a.a.
NCBI ID   WP_013964329.1    Uniprot ID   F8GFC6
Organism   Nitrosomonas sp. Is79A3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 136536..147579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NIT79A3_RS00580 (Nit79A3_0117) - 137121..140348 (+) 3228 WP_013964327.1 type I restriction endonuclease subunit R -
  NIT79A3_RS00585 (Nit79A3_0118) pilU 140366..141502 (-) 1137 WP_013964328.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NIT79A3_RS00590 (Nit79A3_0119) pilT 141536..142579 (-) 1044 WP_013964329.1 type IV pilus twitching motility protein PilT Machinery gene
  NIT79A3_RS00595 (Nit79A3_0120) - 142809..144053 (-) 1245 WP_013964330.1 HDOD domain-containing protein -
  NIT79A3_RS00600 (Nit79A3_0121) - 144290..145132 (-) 843 WP_041360017.1 HDOD domain-containing protein -
  NIT79A3_RS00605 (Nit79A3_0122) - 145393..145644 (-) 252 WP_013964332.1 YfhL family 4Fe-4S dicluster ferredoxin -
  NIT79A3_RS00610 (Nit79A3_0123) coaD 145658..146137 (-) 480 WP_013964333.1 pantetheine-phosphate adenylyltransferase -
  NIT79A3_RS00615 (Nit79A3_0124) rsmD 146130..146684 (-) 555 WP_013964334.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38572.59 Da        Isoelectric Point: 7.5787

>NTDB_id=41634 NIT79A3_RS00590 WP_013964329.1 141536..142579(-) (pilT) [Nitrosomonas sp. Is79A3]
MNIVELLTFVVKNSASDLHLSAGMPPMIRVHGEIRRINLPEMGHKEVHAMVYDIMNDSQRKFYEEHLECDFSFAIPNLAR
FRVNAFNTQRGAAAVMRTIPSKVLSLEDIKAPKIFAEIAKQPRGVVLVTGPTGSGKSTTLAAMINDINENEYGHILTLED
PIEFVHESKKCLINQREVGRDTLSFSNALRSALREDPDIILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAEEKEMMRAMLSESLRAVISQALLRTKDGKGRVAAHEIMIGTPAIRNLIREAKIAQMYSAIQTGQNVGMQTLDQN
LTDLVKRNVISVAEARGKAMNKDNFRI

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=41634 NIT79A3_RS00590 WP_013964329.1 141536..142579(-) (pilT) [Nitrosomonas sp. Is79A3]
ATGAATATAGTTGAGCTGCTTACATTCGTGGTAAAGAACAGCGCTTCAGATTTACATTTATCTGCAGGTATGCCACCGAT
GATCCGGGTACATGGGGAGATCCGCCGCATCAATTTGCCAGAAATGGGTCATAAAGAAGTACACGCTATGGTGTACGACA
TTATGAATGATAGTCAGCGCAAATTTTATGAAGAACACCTGGAGTGTGATTTTTCCTTTGCAATTCCCAATCTTGCACGT
TTCCGCGTCAATGCATTTAATACGCAGCGTGGTGCCGCAGCAGTTATGCGCACGATTCCATCAAAAGTTTTGAGTCTGGA
AGATATCAAAGCGCCCAAAATATTTGCCGAAATTGCTAAGCAACCGCGCGGTGTTGTTTTGGTAACAGGCCCTACCGGCT
CAGGAAAATCCACCACATTAGCGGCAATGATCAATGATATTAACGAGAATGAGTACGGTCACATTCTGACTCTTGAAGAT
CCCATCGAATTTGTGCACGAAAGTAAGAAATGCCTGATTAATCAGCGTGAAGTAGGGCGGGATACCTTAAGCTTTTCCAA
TGCACTGCGTTCGGCGTTGCGTGAAGACCCTGACATCATTCTGGTAGGCGAACTGCGTGATCTGGAAACCATACGCTTGG
CAATGACGGCGGCTGAAACAGGTCATTTAGTATTTGGCACATTGCATACGAGCTCCGCCGCAAAAACCGTGGATCGTATT
ATTGATGTTTTTCCTGCAGAAGAAAAAGAAATGATGCGCGCAATGCTATCCGAATCATTACGCGCTGTTATTTCTCAAGC
GCTGTTAAGAACCAAGGATGGCAAGGGACGCGTTGCAGCACATGAAATAATGATCGGCACACCAGCGATACGTAATTTGA
TCCGCGAAGCCAAAATCGCGCAGATGTATTCGGCAATTCAAACGGGGCAGAACGTTGGCATGCAAACATTGGATCAGAAT
TTAACTGATCTGGTGAAACGCAATGTCATTTCCGTGGCAGAAGCGCGCGGCAAGGCCATGAATAAGGATAATTTCAGGAT
TTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

73.043

99.424

0.726
  pilT Legionella pneumophila strain ERS1305867

72.754

99.424

0.723
  pilT Legionella pneumophila strain Lp02

72.754

99.424

0.723
  pilT Pseudomonas stutzeri DSM 10701

72.174

99.424

0.718
  pilT Pseudomonas aeruginosa PAK

71.884

99.424

0.715
  pilT Neisseria gonorrhoeae MS11

70.145

99.424

0.697
  pilT Neisseria meningitidis 8013

70.145

99.424

0.697
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.568

97.406

0.648
  pilT Vibrio cholerae strain A1552

66.568

97.406

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

99.424

0.49
  pilU Acinetobacter baylyi ADP1

41.714

100

0.421
  pilU Vibrio cholerae strain A1552

40.922

100

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.634

100

0.406

Multiple sequence alignment