Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NIT79A3_RS00585 Genome accession   NC_015731
Coordinates   140366..141502 (-) Length   378 a.a.
NCBI ID   WP_013964328.1    Uniprot ID   F8GFC5
Organism   Nitrosomonas sp. Is79A3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 135366..146502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NIT79A3_RS00570 (Nit79A3_0115) - 135529..135780 (-) 252 WP_013964325.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  NIT79A3_RS00575 (Nit79A3_0116) - 135915..137048 (+) 1134 WP_013964326.1 Fic family protein -
  NIT79A3_RS00580 (Nit79A3_0117) - 137121..140348 (+) 3228 WP_013964327.1 type I restriction endonuclease subunit R -
  NIT79A3_RS00585 (Nit79A3_0118) pilU 140366..141502 (-) 1137 WP_013964328.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NIT79A3_RS00590 (Nit79A3_0119) pilT 141536..142579 (-) 1044 WP_013964329.1 type IV pilus twitching motility protein PilT Machinery gene
  NIT79A3_RS00595 (Nit79A3_0120) - 142809..144053 (-) 1245 WP_013964330.1 HDOD domain-containing protein -
  NIT79A3_RS00600 (Nit79A3_0121) - 144290..145132 (-) 843 WP_041360017.1 HDOD domain-containing protein -
  NIT79A3_RS00605 (Nit79A3_0122) - 145393..145644 (-) 252 WP_013964332.1 YfhL family 4Fe-4S dicluster ferredoxin -
  NIT79A3_RS00610 (Nit79A3_0123) coaD 145658..146137 (-) 480 WP_013964333.1 pantetheine-phosphate adenylyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41865.26 Da        Isoelectric Point: 6.5060

>NTDB_id=41633 NIT79A3_RS00585 WP_013964328.1 140366..141502(-) (pilU) [Nitrosomonas sp. Is79A3]
MDREQATKFMSDLLRLMLSKTASDLFITAGFPPAFKIDGKMTPVSQQSLSAQHTEVLARAIMNDKQAAEFEAAKECNFAI
SPAGIGRFRVNTFVQQQRVGMVLRTITTKIPEFDDLGLPQILKEVVMSKRGLVIFVGGTGSGKSTSMAALIGHRNKNSFG
HIITIEDPVEYVHEHIHCVITQREVGVDTESWEAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCLGTLHANSA
NQALDRIINFFPEERRAQLLMDLSLNMKSLIAQRLIPSKDGKGRVAAIEVLLNSPLIADLIFKGHVHEIKEIMKKSGEMG
MQTFDAALFELYEAGKISYEDALRNADSVNELRLQIKLEGAEAKTRDLSSGIEHLTIT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=41633 NIT79A3_RS00585 WP_013964328.1 140366..141502(-) (pilU) [Nitrosomonas sp. Is79A3]
ATGGATAGGGAACAAGCAACAAAATTTATGTCTGATTTGCTGCGCTTAATGCTCAGCAAAACGGCATCCGACTTATTTAT
TACAGCCGGGTTTCCGCCCGCATTCAAAATTGATGGAAAAATGACACCGGTTTCTCAGCAATCATTGTCTGCACAGCATA
CCGAAGTGTTAGCCAGAGCGATTATGAACGACAAGCAAGCGGCTGAGTTTGAAGCTGCCAAAGAATGTAATTTCGCGATT
AGTCCCGCCGGAATCGGGCGTTTCCGGGTTAACACCTTTGTCCAGCAGCAGCGGGTCGGCATGGTGTTGCGTACGATTAC
AACCAAGATCCCTGAATTTGATGATTTGGGTTTACCGCAGATACTCAAGGAAGTTGTGATGAGCAAGCGGGGTCTGGTGA
TTTTTGTCGGCGGCACCGGTTCGGGAAAATCCACATCGATGGCCGCTTTAATCGGGCACCGCAATAAAAATAGCTTCGGC
CATATTATTACCATTGAAGATCCGGTGGAATATGTGCACGAACACATCCATTGTGTCATTACGCAACGTGAAGTCGGAGT
TGATACCGAATCGTGGGAAGCGGCTTTAAAAAATACACTGCGGCAAGCACCCGATGTCATTTTAATCGGCGAGATACGTG
ATCGTGAAACCATGGAGCATGCCATTGCATTTGCCGAAACCGGCCATCTTTGTTTGGGCACGTTACATGCAAACAGCGCC
AATCAGGCACTTGACCGCATTATCAATTTTTTTCCGGAAGAGCGCAGGGCTCAGTTACTCATGGATTTGTCGTTGAATAT
GAAATCACTGATAGCACAGCGATTGATACCGTCGAAAGATGGAAAAGGACGCGTAGCAGCCATCGAAGTGTTGCTGAACT
CACCACTTATCGCGGATCTGATTTTTAAGGGACATGTCCACGAAATTAAGGAGATTATGAAAAAATCCGGTGAAATGGGC
ATGCAGACCTTTGATGCTGCATTATTTGAGCTTTATGAAGCGGGGAAAATAAGCTATGAAGACGCACTGCGGAACGCTGA
TTCCGTGAATGAGCTACGCTTGCAAATCAAACTCGAAGGCGCAGAAGCCAAGACGCGGGATTTAAGTTCTGGCATAGAGC
ACCTGACAATAACATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(48-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F8GFC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.74

96.561

0.606
  pilU Acinetobacter baylyi ADP1

59.889

94.974

0.569
  pilU Vibrio cholerae strain A1552

56.598

90.212

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

89.153

0.392
  pilT Pseudomonas aeruginosa PAK

42.136

89.153

0.376
  pilT Legionella pneumophila strain Lp02

41.141

88.095

0.362
  pilT Legionella pneumophila strain ERS1305867

41.141

88.095

0.362

Multiple sequence alignment