Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CNE_RS14930 Genome accession   NC_015726
Coordinates   3184059..3185102 (-) Length   347 a.a.
NCBI ID   WP_013957933.1    Uniprot ID   G0EVU4
Organism   Cupriavidus necator N-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3179059..3190102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CNE_RS14900 (CNE_1c30540) - 3180068..3180445 (+) 378 WP_010815044.1 CidA/LrgA family protein -
  CNE_RS14905 (CNE_1c30550) - 3180442..3181167 (+) 726 WP_013957927.1 LrgB family protein -
  CNE_RS14910 (CNE_1c30560) - 3181253..3181624 (+) 372 WP_018311518.1 VOC family protein -
  CNE_RS14915 (CNE_1c30570) - 3181647..3182336 (+) 690 WP_013957929.1 YafY family protein -
  CNE_RS43060 - 3182269..3182415 (+) 147 WP_371827426.1 hypothetical protein -
  CNE_RS14920 (CNE_1c30580) - 3182352..3182837 (-) 486 Protein_2995 glutathione peroxidase -
  CNE_RS14925 (CNE_1c30590) pilU 3182893..3184032 (-) 1140 WP_013957931.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CNE_RS14930 (CNE_1c30600) pilT 3184059..3185102 (-) 1044 WP_013957933.1 type IV pilus twitching motility protein PilT Machinery gene
  CNE_RS14935 (CNE_1c30610) - 3185167..3185862 (+) 696 WP_041228119.1 YggS family pyridoxal phosphate-dependent enzyme -
  CNE_RS14940 (CNE_1c30620) proC 3185919..3186755 (+) 837 WP_013957934.1 pyrroline-5-carboxylate reductase -
  CNE_RS14945 (CNE_1c30630) ubiA 3186824..3187681 (-) 858 WP_041228121.1 4-hydroxybenzoate octaprenyltransferase -
  CNE_RS14950 (CNE_1c30640) - 3187823..3188308 (-) 486 WP_041228123.1 Dps family protein -
  CNE_RS14955 (CNE_1c30650) - 3188401..3188598 (-) 198 WP_013957937.1 hypothetical protein -
  CNE_RS14960 (CNE_1c30660) - 3188649..3190091 (-) 1443 WP_013957938.1 catalase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38367.15 Da        Isoelectric Point: 6.9772

>NTDB_id=41622 CNE_RS14930 WP_013957933.1 3184059..3185102(-) (pilT) [Cupriavidus necator N-1]
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVASMTHKDVHAMVYDIMSDTQRKAYEERLEIDFSFEIAGLSR
FRVNAYNTQRGAAAVFRTIPSKVLTLEELRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNESDMGHILTVED
PIEFVHSSKKSLINQRELGPHTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRSVISYNDARAIAKNPDAFMG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=41622 CNE_RS14930 WP_013957933.1 3184059..3185102(-) (pilT) [Cupriavidus necator N-1]
ATGGACATCGCGCAGCTATTGGCTTTCGCTGTCAAGAACAAGGCGTCTGATCTTCATCTCTCCGCGGACATGCCGCCGAT
GGTGCGGATCCACGGCGACATGCGCCGGATCAACGTCGCCTCGATGACGCACAAGGATGTCCACGCCATGGTGTACGACA
TCATGAGCGACACCCAGCGCAAGGCCTACGAAGAACGCCTCGAAATCGATTTCTCGTTCGAGATCGCCGGCCTGTCCCGC
TTCCGGGTCAATGCCTATAACACCCAGCGCGGCGCCGCCGCGGTGTTCCGTACCATTCCCTCCAAGGTGCTCACGCTCGA
AGAGCTGCGCGCGCCCGCGGTGTTTGCCGACCTGTGCATGAAGCCGCGCGGGCTGGTGCTGGTAACGGGCCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCGATGGTCGACCATCGCAACGAAAGCGACATGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACAGCTCCAAGAAGAGCCTGATCAACCAGCGCGAGCTGGGGCCGCATACGCATTCGTTTGCCAA
CGCGCTGCGCTCGGCACTGCGTGAAGACCCGGACGTGATCCTGGTGGGTGAGCTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTCTTCGGCACGCTGCACACCAGCTCCGCGGCCAAGACCATCGACCGGGTG
GTCGATGTGTTCCCGCCCGAAGAGAAGGACATGGTGCGCACCATGCTGTCGGAATCGCTGGAGGCGGTGATCTCGCAGAC
GCTGCTGAAGACGCGCGACGGCAATGGCCGCACCGCCGCGCACGAGATCATGATCGCCACGCCCGCGATCCGCCACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAGTGC
CTGTCTGACCTGATCAAGCGCAGCGTCATCAGCTACAACGACGCGCGCGCCATCGCGAAGAACCCCGACGCGTTCATGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0EVU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

71.676

99.712

0.715
  pilT Acinetobacter baumannii strain A118

71.676

99.712

0.715
  pilT Acinetobacter nosocomialis M2

71.676

99.712

0.715
  pilT Pseudomonas stutzeri DSM 10701

69.855

99.424

0.695
  pilT Pseudomonas aeruginosa PAK

69.855

99.424

0.695
  pilT Acinetobacter baylyi ADP1

69.565

99.424

0.692
  pilT Legionella pneumophila strain Lp02

67.246

99.424

0.669
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Neisseria meningitidis 8013

67.246

99.424

0.669
  pilT Legionella pneumophila strain ERS1305867

67.246

99.424

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.172

97.118

0.643
  pilT Vibrio cholerae strain A1552

66.172

97.118

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

99.424

0.493
  pilU Acinetobacter baylyi ADP1

40.116

99.135

0.398
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392
  pilU Pseudomonas stutzeri DSM 10701

40.299

96.542

0.389

Multiple sequence alignment