Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CNE_RS14925 Genome accession   NC_015726
Coordinates   3182893..3184032 (-) Length   379 a.a.
NCBI ID   WP_013957931.1    Uniprot ID   G0EVU3
Organism   Cupriavidus necator N-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3177893..3189032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CNE_RS14895 (CNE_1c30530) glcF 3178736..3179989 (+) 1254 WP_013957926.1 glycolate oxidase subunit GlcF -
  CNE_RS14900 (CNE_1c30540) - 3180068..3180445 (+) 378 WP_010815044.1 CidA/LrgA family protein -
  CNE_RS14905 (CNE_1c30550) - 3180442..3181167 (+) 726 WP_013957927.1 LrgB family protein -
  CNE_RS14910 (CNE_1c30560) - 3181253..3181624 (+) 372 WP_018311518.1 VOC family protein -
  CNE_RS14915 (CNE_1c30570) - 3181647..3182336 (+) 690 WP_013957929.1 YafY family protein -
  CNE_RS43060 - 3182269..3182415 (+) 147 WP_371827426.1 hypothetical protein -
  CNE_RS14920 (CNE_1c30580) - 3182352..3182837 (-) 486 Protein_2995 glutathione peroxidase -
  CNE_RS14925 (CNE_1c30590) pilU 3182893..3184032 (-) 1140 WP_013957931.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CNE_RS14930 (CNE_1c30600) pilT 3184059..3185102 (-) 1044 WP_013957933.1 type IV pilus twitching motility protein PilT Machinery gene
  CNE_RS14935 (CNE_1c30610) - 3185167..3185862 (+) 696 WP_041228119.1 YggS family pyridoxal phosphate-dependent enzyme -
  CNE_RS14940 (CNE_1c30620) proC 3185919..3186755 (+) 837 WP_013957934.1 pyrroline-5-carboxylate reductase -
  CNE_RS14945 (CNE_1c30630) ubiA 3186824..3187681 (-) 858 WP_041228121.1 4-hydroxybenzoate octaprenyltransferase -
  CNE_RS14950 (CNE_1c30640) - 3187823..3188308 (-) 486 WP_041228123.1 Dps family protein -
  CNE_RS14955 (CNE_1c30650) - 3188401..3188598 (-) 198 WP_013957937.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42097.35 Da        Isoelectric Point: 6.6384

>NTDB_id=41621 CNE_RS14925 WP_013957931.1 3182893..3184032(-) (pilU) [Cupriavidus necator N-1]
MLDRESAAKYINDLLELMVSNRGSDLFITSDFPPAIKVDGKIKPVSQQPLNPTQALGLVRSVMNERQVQDFDTSRECNFA
ITAPKAGRFRVSAFIQQGKAGMVVRTINTRIPSVADLDLPATLHEVVMAKRGLVIVTGATGSGKSTTLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRVVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMVSFDQALFDLYEEGKITYEDALRNADSLNDLRLMIKLHSASAKDTDLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=41621 CNE_RS14925 WP_013957931.1 3182893..3184032(-) (pilU) [Cupriavidus necator N-1]
ATGCTCGATCGCGAATCCGCCGCCAAGTACATCAACGACCTGCTGGAGCTGATGGTCAGCAACCGCGGCTCTGACCTGTT
CATCACTTCTGACTTCCCGCCGGCGATCAAGGTCGACGGCAAGATCAAGCCGGTGTCGCAGCAGCCGCTGAACCCGACCC
AGGCGCTGGGCCTGGTGCGCTCGGTGATGAACGAGCGCCAGGTGCAGGACTTCGACACCAGCCGCGAATGCAATTTCGCG
ATCACCGCGCCCAAGGCCGGGCGCTTCCGCGTGTCGGCCTTTATCCAGCAGGGCAAGGCCGGCATGGTGGTGCGCACCAT
CAATACGCGCATCCCCTCGGTGGCCGACCTGGACCTGCCGGCTACGCTGCATGAGGTGGTGATGGCCAAGCGCGGGCTGG
TGATCGTGACCGGTGCCACCGGCTCGGGCAAGTCGACCACGCTGGCGGCAATGCTGGATCACCGCAATGCGCATTCGTAC
GGCCATATCATCACCATCGAGGATCCGATCGAGTACGTGCACGCGCACCAGAACTGCATCGTCACGCAGCGCGAGGTCGG
CATCGATACCGAGTCCTGGCACGTGGCGCTGAAGAACACGTTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCC
GCGATCGCGAGACCATGGAGTACGCGATGCAGTACGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAAC
GCCAACCAGGCGATCGACCGCGTGGTCAACTTCTTCCCCGAGGAAAAGCGCCAGCAGCTGCTGATCGACCTGTCGCTGAA
CCTGAAGGCGATGATCTCGCAGCGCCTGCTGCCGCGCGCGGGCCGCAAAGGGCGGGTGCCGGCGGTGGAGATCATGATCG
GCACGCCGCTGGTGGCCGACCTGATCTTCAAGGGCGAGATCCACGAGCTCAAGGAAGTCATCAAGAAGTCGCGCGAGCAG
GGCATGGTCTCGTTCGACCAGGCGCTGTTCGACCTGTACGAGGAAGGCAAGATCACCTACGAGGACGCGCTGCGCAACGC
CGACTCGCTCAACGACCTGCGCCTGATGATCAAGCTGCACAGCGCGAGCGCCAAGGATACCGACCTGGGTGCCGGCACCG
AGCATCTCAACGTCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0EVU3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.571

92.348

0.578
  pilU Acinetobacter baylyi ADP1

56.825

94.723

0.538
  pilU Vibrio cholerae strain A1552

53.444

95.778

0.512
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

88.918

0.401
  pilT Pseudomonas stutzeri DSM 10701

43.323

88.918

0.385
  pilT Pseudomonas aeruginosa PAK

43.323

88.918

0.385
  pilT Acinetobacter baumannii D1279779

42.943

87.863

0.377
  pilT Acinetobacter nosocomialis M2

42.943

87.863

0.377
  pilT Acinetobacter baumannii strain A118

42.943

87.863

0.377
  pilT Acinetobacter baylyi ADP1

42.643

87.863

0.375
  pilT Legionella pneumophila strain Lp02

42.342

87.863

0.372
  pilT Legionella pneumophila strain ERS1305867

42.342

87.863

0.372
  pilT Neisseria meningitidis 8013

40.294

89.71

0.361

Multiple sequence alignment