Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DNR44_RS13600 Genome accession   NZ_CP047673
Coordinates   2715226..2716272 (-) Length   348 a.a.
NCBI ID   WP_112223729.1    Uniprot ID   A0A365KTP3
Organism   Planococcus halotolerans strain Y50     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2710226..2721272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DNR44_RS13575 (DNR44_013520) - 2710773..2711711 (-) 939 WP_135815760.1 LysM peptidoglycan-binding domain-containing protein -
  DNR44_RS13580 (DNR44_013525) - 2711704..2712450 (-) 747 WP_112223733.1 fimbrial assembly protein -
  DNR44_RS13585 (DNR44_013530) pilM 2712455..2713393 (-) 939 WP_112223732.1 type IV pilus biogenesis protein PilM -
  DNR44_RS13590 (DNR44_013535) - 2713416..2713880 (-) 465 WP_112223731.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DNR44_RS13595 (DNR44_013540) - 2714013..2715224 (-) 1212 WP_112223730.1 type II secretion system F family protein -
  DNR44_RS13600 (DNR44_013545) pilT 2715226..2716272 (-) 1047 WP_112223729.1 type IV pilus twitching motility protein PilT Machinery gene
  DNR44_RS13605 (DNR44_013550) - 2716276..2717934 (-) 1659 WP_205854541.1 GspE/PulE family protein -
  DNR44_RS13610 (DNR44_013555) - 2717957..2719324 (-) 1368 WP_165843784.1 VanW family protein -
  DNR44_RS13615 (DNR44_013560) - 2719326..2719751 (-) 426 WP_112223726.1 hypothetical protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38258.02 Da        Isoelectric Point: 6.5049

>NTDB_id=415193 DNR44_RS13600 WP_112223729.1 2715226..2716272(-) (pilT) [Planococcus halotolerans strain Y50]
MLTTTTFIDNVLTAAKELNVSDIHLTTGIPPVFRMHGTLRRYGEEIITPDHSEAIGKAIMPESLWETFLEKGEMDYSYAI
AGVGRYRVNTFHQRGSISHAFRAIPTVVPTVDDLKMPQILKKLAEIHQGLILVTGPTGSGKSTTLAAMIRHINEEMTKHI
ITLEDPIEYLHRHGTSIIDQREVGFDTNSFANGLRAALRQDPDVILVGEMRDLETIMTAITAAETGHLVMGTLHTSSAAT
TIERIIDVFPPEQQSQIRTQLGGILKAVVAQRLLPTADGKGRVAATEILISNPAVANLIRTEKVHQIPNVILTNRAAGMH
MMSTSVQELLAQGRITRQIAQPFLEGEE

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=415193 DNR44_RS13600 WP_112223729.1 2715226..2716272(-) (pilT) [Planococcus halotolerans strain Y50]
ATGCTGACTACTACAACATTTATCGATAATGTTTTGACTGCAGCCAAGGAATTGAATGTTTCCGATATCCATTTGACCAC
GGGTATCCCACCGGTTTTCCGGATGCATGGCACATTGCGCCGTTACGGAGAAGAAATTATCACACCTGATCATTCAGAAG
CTATCGGCAAAGCTATCATGCCGGAATCGTTATGGGAAACGTTCCTTGAAAAAGGAGAGATGGATTATTCCTACGCAATT
GCTGGAGTCGGCCGTTACCGGGTCAACACTTTCCATCAGCGCGGCTCGATTTCACACGCATTCCGTGCGATTCCAACCGT
GGTTCCGACAGTCGATGATTTGAAAATGCCGCAGATTTTAAAGAAACTGGCAGAAATTCATCAGGGACTTATCCTGGTAA
CTGGCCCTACTGGTTCAGGTAAATCAACGACGCTAGCGGCAATGATTCGTCATATCAATGAAGAAATGACGAAACATATC
ATTACGCTGGAGGATCCGATTGAGTATCTTCATAGACACGGCACATCGATTATCGATCAACGCGAAGTCGGATTCGATAC
GAACAGTTTTGCCAACGGATTACGCGCAGCGCTTCGACAGGATCCGGATGTAATTCTGGTCGGTGAGATGCGTGACCTGG
AAACGATTATGACCGCTATCACCGCCGCTGAAACGGGTCACTTGGTTATGGGTACGCTGCATACATCGAGTGCCGCTACA
ACAATCGAGCGGATTATTGACGTCTTTCCGCCAGAACAGCAATCTCAGATTCGGACGCAGCTTGGCGGTATTTTGAAAGC
TGTTGTTGCTCAACGGTTATTGCCGACAGCAGATGGCAAGGGTCGAGTCGCTGCAACTGAAATCTTGATTTCAAATCCGG
CAGTCGCCAACTTAATCCGTACAGAAAAAGTTCACCAGATACCGAACGTAATCCTGACCAACCGGGCAGCCGGTATGCAT
ATGATGTCGACTTCCGTCCAGGAACTTTTGGCTCAAGGCAGGATAACCAGACAAATCGCTCAACCTTTCCTGGAAGGAGA
GGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A365KTP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.642

96.264

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.07

93.966

0.48
  pilT Vibrio cholerae strain A1552

51.07

93.966

0.48
  pilT Neisseria meningitidis 8013

49.102

95.977

0.471
  pilT Neisseria gonorrhoeae MS11

48.802

95.977

0.468
  pilT Acinetobacter baylyi ADP1

48.943

95.115

0.466
  pilT Pseudomonas stutzeri DSM 10701

47.605

95.977

0.457
  pilT Acinetobacter baumannii strain A118

49.221

92.241

0.454
  pilT Acinetobacter baumannii D1279779

49.221

92.241

0.454
  pilT Acinetobacter nosocomialis M2

48.91

92.241

0.451
  pilT Legionella pneumophila strain Lp02

48.297

92.816

0.448
  pilT Legionella pneumophila strain ERS1305867

48.297

92.816

0.448
  pilT Pseudomonas aeruginosa PAK

46.108

95.977

0.443
  pilU Pseudomonas stutzeri DSM 10701

42.812

91.954

0.394
  pilU Vibrio cholerae strain A1552

40.12

95.977

0.385
  pilU Acinetobacter baylyi ADP1

38.182

94.828

0.362

Multiple sequence alignment