Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GRX76_RS01805 Genome accession   NZ_CP047569
Coordinates   463259..464293 (+) Length   344 a.a.
NCBI ID   WP_160151731.1    Uniprot ID   -
Organism   Microbulbifer sp. ALW1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 458259..469293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRX76_RS01785 - 458658..459263 (-) 606 WP_160151728.1 YggT family protein -
  GRX76_RS01790 proC 459275..460102 (-) 828 WP_236250509.1 pyrroline-5-carboxylate reductase -
  GRX76_RS01795 - 460303..462399 (+) 2097 WP_160151729.1 alpha/beta fold hydrolase -
  GRX76_RS01800 - 462464..463159 (-) 696 WP_160151730.1 YggS family pyridoxal phosphate-dependent enzyme -
  GRX76_RS01805 pilT 463259..464293 (+) 1035 WP_160151731.1 type IV pilus twitching motility protein PilT Machinery gene
  GRX76_RS01810 pilU 464531..465679 (+) 1149 WP_160151732.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GRX76_RS01815 - 465752..466507 (+) 756 WP_160151733.1 SDR family oxidoreductase -
  GRX76_RS01820 - 466536..468212 (+) 1677 WP_160151734.1 amidohydrolase -
  GRX76_RS01825 - 468221..468988 (-) 768 WP_160151735.1 LytTR family DNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38441.05 Da        Isoelectric Point: 6.5789

>NTDB_id=414209 GRX76_RS01805 WP_160151731.1 463259..464293(+) (pilT) [Microbulbifer sp. ALW1]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHGLIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGQVFKQISDTPRGLVLVTGPTGSGKSTSLAAMIDYINDNKYEHILTVED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAQEKAMVRSMLSESLQAVISQTLLKRNGGGRVAAHEIMRGTPAIRNLIREDKIAQMYSAIQTGANVGMQTMDQCL
QELVEKRIISRETAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=414209 GRX76_RS01805 WP_160151731.1 463259..464293(+) (pilT) [Microbulbifer sp. ALW1]
ATGGACATTACAGAACTCCTCGCCTTCAGTGCCAAGCAGAACGCCTCCGACTTGCACCTCTCCGCGGGCCTGCCACCCAT
GATCCGGGTCGATGGCGACGTGCGCCGTATCAACCTGCCGCCCATGGAGCACAAGCAGGTGCACGGCCTGATCTACGAGA
TCATGAACGACAAGCAGCGCAAGGACTACGAAGAGTTCCTCGAGACCGACTTCTCCTTCGAAGTCCCCGGTGTCGCCCGC
TTCCGTGTGAACGCCTTCAACCACAACCGCGGCGCCGGCGCCGTATTCCGGACCATTCCCTCCAAGGTACTGACCATGGA
AGATCTCGGAATGGGCCAGGTGTTCAAGCAGATCTCCGACACTCCCCGCGGCCTGGTACTGGTCACCGGCCCCACCGGCT
CCGGTAAGTCCACCTCACTGGCCGCGATGATCGACTACATCAACGACAACAAATACGAGCATATCCTCACCGTCGAAGAC
CCGATCGAATTCGTGCACGAATCCAAAAAGTGCCTGGTCAACCAGCGGGAAGTGCACCGCGACACCCACGGCTTCTCCGA
AGCCCTGCGCTCCGCACTGCGTGAAGACCCGGACATCATCCTCGTGGGTGAGATGCGAGACCTCGAAACCATCCGCCTGG
CACTGACCGCCGCGGAAACCGGCCACCTGGTATTCGGCACCCTGCACACCACCTCCGCCGCCAAAACCATCGACCGGGTT
GTCGACGTATTCCCGGCGCAGGAAAAAGCCATGGTGCGCTCCATGCTGTCGGAATCCCTGCAGGCGGTAATTTCCCAGAC
CCTGCTCAAGCGCAATGGCGGCGGCCGTGTCGCCGCCCACGAAATCATGCGCGGCACCCCGGCCATCCGGAACCTCATCC
GCGAAGATAAAATCGCGCAGATGTACTCCGCCATCCAGACCGGTGCCAACGTGGGTATGCAGACCATGGACCAGTGCCTG
CAGGAACTGGTGGAGAAACGTATCATCAGCCGCGAAACCGCGCGCGAAAAAGCGAAAATGCCAGAAAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.953

100

0.89
  pilT Pseudomonas stutzeri DSM 10701

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

84.302

100

0.843
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter baylyi ADP1

80.523

100

0.805
  pilT Legionella pneumophila strain Lp02

76.331

98.256

0.75
  pilT Legionella pneumophila strain ERS1305867

76.331

98.256

0.75
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.647

98.837

0.669
  pilT Vibrio cholerae strain A1552

67.647

98.837

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

40.118

98.547

0.395
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39

Multiple sequence alignment